In vitro inhibition of sole aquabirnavirus by Senegalese sole Mx

Fernández-Trujillo, M.A.; García-Rosado, Esther; Alonso, M. Carmen; Borrego, Juan J.; Álvarez, M. Carmen; Béjar, Julia

doi:10.1016/j.fsi.2007.10.010

Cited by 19 publications

(10 citation statements)

References 38 publications

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“…Mx proteins are IFN-induced proteins that are widespread in eukaryotes, however, their antiviral activity is unclear and the results variable among species [48]. Therefore, assessment of the putative Mx antiviral activity in each species is of interest.…”

Section: Discussionmentioning

confidence: 99%

Protective Roles of Grass Carp Ctenopharyngodon idella Mx Isoforms against Grass Carp Reovirus

Peng

Yang

2012

PLoS ONE

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BackgroundMyxovirus resistance (Mx) proteins are crucial effectors of the innate antiviral response against a wide range of viruses, mediated by the type I interferon (IFN-I) signaling pathway. However, the antiviral activity of Mx proteins is diverse and complicated in different species.Methodology/Principal FindingsIn the current study, two novel Mx genes (CiMx1 and CiMx3) were identified in grass carp (Ctenopharyngodon idella). CiMx1 and CiMx3 proteins exhibit high sequence identity (92.1%), and low identity with CiMx2 (49.2% and 49.5%, respectively) from the GenBank database. The predicted three-dimensional (3D) structures are distinct among the three isoforms. mRNA instability motifs also display significant differences in the three genes. The spatial and temporal expression profiles of three C. idella Mx genes and the IFN-I gene were investigated by real-time fluorescence quantitative RT-PCR (qRT-PCR) following infection with grass carp reovirus (GCRV) in vivo and in vitro. The results demonstrated that all the four genes were implicated in the anti-GCRV immune response, that mRNA expression of Mx genes might be independent of IFN-I, and that CIK cells are suitable for antiviral studies. By comparing expression patterns following GCRV challenge or poly(I:C) treatment, it was observed that GCRV blocks mRNA expression of the four genes. To determine the functions of Mx genes, three CiMx cDNAs were cloned into expression vectors and utilized for transfection of CIK cells. The protection conferred by each recombinant CiMx protein against GCRV infection was evaluated. Antiviral activity against GCRV was demonstrated by reduced cytopathic effect, lower virus titer and lower levels of expressed viral transcripts. The transcription of IFN-I gene was also monitored.Conclusions/SignificanceThe results indicate all three Mx genes can suppress replication of grass carp reovirus and over-expression of Mx genes mediate feedback inhibition of the IFN-I gene.

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Section: Discussionmentioning

confidence: 99%

Protective Roles of Grass Carp Ctenopharyngodon idella Mx Isoforms against Grass Carp Reovirus

Peng

Yang

2012

PLoS ONE

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“…Mx protein, an interferon‐induced protein that protects against viral infections, has also been studied in Senegalese sole (Fernández‐Trujillo et al . ). Fernández‐Trujillo et al .…”

Section: Health Issuesmentioning

confidence: 97%

New developments and biological insights into the farming of Solea senegalensis reinforcing its aquaculture potential

Morais

Aragão

Cabrita

et al. 2014

Reviews in Aquaculture

116

108

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Senegalese sole was one of the earliest identified candidate species with high potential for aquaculture diversification in the south of Europe. Its culture has been possible, and commercially attempted, for several decades, but intensive production has been slow to take off. This has been explained mostly by serious disease problems, high mortality at weaning, variable growth and poor juvenile quality. However, a strong and sustained research investment that started in the eighties has led to a better understanding of the requirements and particularities of this species. More recently, better management and technical improvements have been introduced, which have led to important progress in productivity and given a new impetus to the cultivation of Senegalese sole. As a result, the last 5 years have marked a probable turning point in the culture of sole towards the development of a knowledge-driven, competitive and sustainable industry. This review will focus on the main technical improvements and advances in the state of knowledge that have been made in the last decade in areas as diverse as reproductive biology, behaviour, physiology, nutritional requirements, modulation of the immune system in response to environmental parameters and stress, and characterization and mitigation of the main disease threats. It is now clear that Senegalese sole has important particularities that differentiate it from other current and candidate marine aquaculture species, which bring about important challenges, some still unsolved, but also notable opportunities (e.g. a nutritional physiology that is better adapted to dietary vegetable ingredients), as will be discussed here.

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“…Furthermore, it has been demonstrated by functional analyses that some of fish Mx proteins themselves had antiviral activity [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression analysis of three Mx isoforms of rock bream, Oplegnathus fasciatus

Zenke

Kim

2009

Fish & Shellfish Immunology

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In vitro inhibition of sole aquabirnavirus by Senegalese sole Mx

Cited by 19 publications

References 38 publications

Protective Roles of Grass Carp Ctenopharyngodon idella Mx Isoforms against Grass Carp Reovirus

Protective Roles of Grass Carp Ctenopharyngodon idella Mx Isoforms against Grass Carp Reovirus

New developments and biological insights into the farming of Solea senegalensis reinforcing its aquaculture potential

Molecular cloning and expression analysis of three Mx isoforms of rock bream, Oplegnathus fasciatus

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