Cloning and expression analysis of tyrosine hydroxylase and changes in catecholamine levels in brain during ontogeny and after sex steroid analogues exposure in the catfish, Clarias batrachus

Mamta, Sajwan-Khatri; Kavarthapu, Raghuveer; Sudhakumari, C.C.; Anbazhagan, Ramaswamy; Basavaraju, Y.; Senthilkumaran, B.

doi:10.1016/j.ygcen.2013.11.022

Cited by 27 publications

(21 citation statements)

References 41 publications

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“…Brain Th ontogeny also revealed higher transcript levels in female brain at 50, 75 and 100 dph as compared to male brain at same age groups (Mamta et al, 2014; Table 1) wherein 50 dph is the critical period for gonadal sex differentiation in catfish (Raghuveer et al, 2011a). These findings were further correlated with the levels of NE and DA which also showed sexual dimorphism (Mamta et al, 2014). Interestingly in wrasse, CAs play an essential role in the initiation and completion of sex reversal (Larson et al, 2003).…”

Section: Hypothalamic Monoamines and Related Enzymes' Expressionmentioning

confidence: 79%

“…Clarias batrachus (catfish) Th Mamta et al (2014) Female specific high expression of Th in brain during early development 3…”

Section: Speciesmentioning

confidence: 99%

“…Though epinephrine has been detected with seasonal differences, no role has been precisely attributed to its function except to implicate stimulatory effect on GTH as that of NE (Senthilkumaran and Joy, 1995). To delineate female brain sex development/differentiation, in depth studies on the development of the CAergic system during male and female brain sex development of air-breathing catfish, Clarias batrachus demonstrated gender specific differences where female brain showed higher level of tyrosine hydroxylase (Th) expression with high copy numbers as compared to males (Mamta et al, 2014; Table 1). Brain Th ontogeny also revealed higher transcript levels in female brain at 50, 75 and 100 dph as compared to male brain at same age groups (Mamta et al, 2014; Table 1) wherein 50 dph is the critical period for gonadal sex differentiation in catfish (Raghuveer et al, 2011a).…”

Section: Hypothalamic Monoamines and Related Enzymes' Expressionmentioning

confidence: 99%

“…To delineate female brain sex development/differentiation, in depth studies on the development of the CAergic system during male and female brain sex development of air-breathing catfish, Clarias batrachus demonstrated gender specific differences where female brain showed higher level of tyrosine hydroxylase (Th) expression with high copy numbers as compared to males (Mamta et al, 2014; Table 1). Brain Th ontogeny also revealed higher transcript levels in female brain at 50, 75 and 100 dph as compared to male brain at same age groups (Mamta et al, 2014; Table 1) wherein 50 dph is the critical period for gonadal sex differentiation in catfish (Raghuveer et al, 2011a). These findings were further correlated with the levels of NE and DA which also showed sexual dimorphism (Mamta et al, 2014).…”

Section: Hypothalamic Monoamines and Related Enzymes' Expressionmentioning

confidence: 99%

See 3 more Smart Citations

“Brain sex differentiation” in teleosts: Emerging concepts with potential biomarkers

Senthilkumaran¹,

Sudhakumari²,

Mamta³

et al. 2015

General and Comparative Endocrinology

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Section: Hypothalamic Monoamines and Related Enzymes' Expressionmentioning

confidence: 79%

“…Clarias batrachus (catfish) Th Mamta et al (2014) Female specific high expression of Th in brain during early development 3…”

Section: Speciesmentioning

confidence: 99%

Section: Hypothalamic Monoamines and Related Enzymes' Expressionmentioning

confidence: 99%

Section: Hypothalamic Monoamines and Related Enzymes' Expressionmentioning

confidence: 99%

See 2 more Smart Citations

“Brain sex differentiation” in teleosts: Emerging concepts with potential biomarkers

Senthilkumaran¹,

Sudhakumari²,

Mamta³

et al. 2015

General and Comparative Endocrinology

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“…It seems that alterations in both sexes could be intepreted via a drop in the reserve pool since the activity or density of the surfaceexpressed DATs were not significantly influenced (compare Tables 1, 2). Since our previous experiments performed on 21-day old male and female pups either coexposed to MA prenatally in utero and postnatally via breast milk (MA/MA groups) or exposed to MA only postnatally via breast milk (S/MA groups) did not reveal significant sex-differences in MA brain concentrations [8], it seems probable that MA-evoked differences between preadolescent males and females in striatal DATs could reflect gender-specific ontogenetic development of the dopaminergic neurotransmitter system, including its maturation, and could be based among others on the actions of sex steroid hormones [43][44][45][46][47][48][49][50][51]. E.g., experiments on mesencephalic and diencephalic rat embryonic cells revealed higher levels of DA in females compared to males at gestation day 14 [45] and subtle sex differences in numbers and distribution of TH-immunoreactive neurons at gestation day 17 [44].…”

Section: Datmentioning

confidence: 99%

Sex-Dependent Changes in Striatal Dopamine Transport in Preadolescent Rats Exposed Prenatally and/or Postnatally to Methamphetamine

et al. 2016

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Methamphetamine (MA) is the most commonly used psychostimulant drug, the chronic abuse of which leads to neurodegenerative changes in the brain. The global use of MA is increasing, including in pregnant women. Since MA can cross both placental and haematoencephalic barriers and is also present in maternal milk, children of chronically abused mothers are exposed prenatally as well as postnatally. Women seem to be more vulnerable to some aspects of MA abuse than men. MA is thought to exert its effects among others via direct interactions with dopamine transporters (DATs) in the brain tissue. Sexual dimorphism of the DAT system could be a base of sex-dependent actions of MA observed in behavioural and neurochemical studies. Possible sex differences in the DATs of preadolescent offspring exposed to MA prenatally and/or postnatally have not yet been evaluated. We examined the striatal synaptosomal DATs (the activity and density of surface expressed DATs and total DAT expression) in preadolescent male and female Wistar rats (31-35-day old animals) exposed prenatally and/or postnatally to MA (daily 5 mg/kg, s.c. to mothers during pregnancy and lactation). To distinguish between specific and nonspecific effects of MA on DATs, we also evaluated the in vitro effects of lipophilic MA on the fluidity of striatal membranes isolated from preadolescent and young adult rats of both sexes. We observed similar changes in the DATs of preadolescent rats exposed prenatally or postnatally (MA-mediated drop in the reserve pool but no alterations in surface-expressed DATs). However, prenatal exposure evoked significant changes in males and postnatal exposure in females. A significant decrease in the activity of surface-expressed DATs was found only in postnatally exposed females sensitized to MA via prenatal exposure. MA applied in vitro increased the fluidity of striatal membranes of preadolescent female but not male rats. In summary, DATs of preadolescent males are more sensitive to prenatal MA exposure via changes in the reserve pool and those of preadolescent females to postnatal MA exposure via the same mechanism. The combination of prenatal and postnatal MA exposure increases the risk of dopaminergic deficits via alterations in the activity of surface-expressed DATs especially in preadolescent females. MA-mediated changes in DATs of preadolescent females could be still enhanced via nonspecific disordering actions of MA on striatal membranes.

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Secretagogin in the brain and pituitary of the catfish, Clarias batrachus: Molecular characterization and regulation by insulin

Basu

Mitra

Singh

et al. 2022

J of Comparative Neurology

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Secretagogin (scgn), is a novel hexa EF-hand, phylogenetically conserved calciumbinding protein. It serves as Ca 2+ sensor and participates in Ca 2+ -signaling and neuroendocrine regulation in mammals. However, its relevance in the brain of nonmammalian vertebrates has largely remained unexplored. To address this issue, we studied the cDNA encoding scgn, scgn mRNA expression, and distribution of scgnequipped elements in the brain and pituitary of a teleost, Clarias batrachus (cb). The cbscgn cDNA consists of three transcripts (T) variants: T1 (2185 bp), T2 (2151 bp) and T3 (2060 bp). While 816 bp ORF in T1 and T2 encodes highly conserved six EF-hand 272 aa protein fully capable of Ca 2+ -binding, 726-bp ORF in T3 encodes 242 aa protein.The T1 showed >90% and >70% identity with scgn of catfishes, and other teleosts and mammals, respectively. The T1-mRNA was widely expressed in the brain and pituitary, while the expression of T3 was restricted to the telencephalon. Application of the antiscgn antiserum revealed a ∼32 kDa scgn-immunoreactive (scgn-i) band (known molecular weight of scgn) in the forebrain tissue, and immunohistochemically labeled neurons in the olfactory epithelium and bulb, telencephalon, preoptic area, hypothalamus, thalamus, and hindbrain. In the pituitary, scgn-i cells were seen in the pars distalis and intermedia. Insulin is reported to regulate scgn mRNA in the mammalian hippocampus, and feeding-related neuropeptides in the telencephalon of teleost. Intracranial injection of insulin significantly increased T1-mRNA expression and scgn-immunoreactivity

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Cloning and expression analysis of tyrosine hydroxylase and changes in catecholamine levels in brain during ontogeny and after sex steroid analogues exposure in the catfish, Clarias batrachus

Cited by 27 publications

References 41 publications

“Brain sex differentiation” in teleosts: Emerging concepts with potential biomarkers

“Brain sex differentiation” in teleosts: Emerging concepts with potential biomarkers

Sex-Dependent Changes in Striatal Dopamine Transport in Preadolescent Rats Exposed Prenatally and/or Postnatally to Methamphetamine

Secretagogin in the brain and pituitary of the catfish, Clarias batrachus: Molecular characterization and regulation by insulin

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