Cloning and Characterization of the Constitutively Expressed Chitinase C Gene from a Marine Bacterium, Salinivibrio costicola Strain 5SM-1

Aunpad, Ratchaneewan; Panbangred, Watanalai

doi:10.1263/jbb.96.529

Cited by 9 publications

(15 citation statements)

References 31 publications

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“…Its chitinases were significantly produced in the absence and at high salt concentration of 5-15 NaCl% (w/ v), which clearly indicated the highly salt-tolerant nature of the enzyme. By comparison with few studies on halo-tolerance of chitinase enzymes, the same results, of chitinase function in the absence of NaCl has been proved with other chitinases produced from halophilic bacteria, such as Salinivibrio costicola 5SM-1 (Aunpad and Panbangred 2003) as well as by the marine bacteria Vibrio harveyi and Alteromonas sp. strain O-7 (Sivitil and Kirchman 1998;Tsujibo et al 1999).…”

Section: Discussionsupporting

confidence: 57%

Production and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26

Essghaier

Rouaissi

Boudabous

et al. 2009

World J Microbiol Biotechnol

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This paper is the first to investigate the production and partial characterization of the chitinase enzyme from a moderately halophilic bacterium Planococcus rifitoensis strain M2-26, earlier isolated from a shallow salt lake in Tunisia. The impact of salt, salinity concentration, pH, carbon and nitrogen sources on chitinase production and activity have been determined. This is the first report on a high salt-tolerant chitinase from P. rifitoensis, since it was active at high salinity (from 5 to 30% NaCl) as well as in the absence of salt. This enzyme showed optimal activity at 70°C and retained up to 82 and 66% of its original activity at 80 or 90°C, respectively. The activity of the enzyme was also shown over a wide pH range (from 5 to 11). For characterization of the enzyme activity, the chitinase secreted in the culture supernatant was partially purified. The preliminary study of the concentrated dialysed supernatant on native PAGE showed at least three chitinases produced by strain M2-26, with highest activity approximately at 65 kDa. Thus, the thermo-tolerant and high salt-tolerant chitinases produced by P. rifitoensis strain M2-26 could be useful for application in diverse areas such as biotechnology and agro-industry.

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Section: Discussionsupporting

confidence: 57%

Production and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26

Essghaier

Rouaissi

Boudabous

et al. 2009

World J Microbiol Biotechnol

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“…Chitinase of halotolerant B. subtilis strain J9 was significantly produced in the absence and at high salt concentration of 0 to 25% NaCl (w/v) which clearly indicated the highly salt-tolerant nature of the enzyme. By comparison with few studies on halo-tolerance of chitinase enzymes, the same results of salt tolerant chitinase has been proved with other chitinases produced from halophilic bacteria such as Salinivibrio costicola 5SM-1 (Aunpad and Panbangred, 2003), Planococcus rifitoensis M2-26 (Essghaier et al, 2010) as well as by the marine bacteria Vibrio harveyi and Alteromonas sp. strain O-7 (Tsujibo et al, 1999).…”

Section: Antifungal Chitinase Characterizationsupporting

confidence: 54%

In vivo and in vitro evaluation of antifungal activities from a halotolerant Bacillus subtilis strain J9

Essghaier¹,

Hedi²,

Hajlaoui³

et al. 2012

Afr. J. Microbiol. Res.

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In this study, we aimed to identify the antifungal activities of the halotolerant Bacillus subtilis strain J9 under different carbon sources and salinity conditions in vitro and in vivo under field conditions on strawberry. The strain J9 of B. subtilis was able to reduce grey mold disease on strawberry under field conditions with percentage reduction ranging from 46.3 to 44.3% which was comparable to those of chemical fungicides respectively after treatments of 8 and 40 days. Therefore, the strain J9 may be a good candidate for biological control. The strain J9 was able to produce high antifungal enzymes inducible by the pathogen; chitinase (102 U/ml), protease (14.5 U/ml) and laminarinase (0.74 U/ml). Furthermore, high halotolerant chitinase was detected in the culture supernatant under 0 to 25% NaCl (w/v). Interestingly, we described distinguishable characteristics of high activity and stability at different pH, temperatures of B. subtilis J9 chitinase. The analysis of the amino acid sequences coding to the putative catalytic domain from enzyme highlighted its relation to those of glycosyl hydrolase family 18.

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“…After incubation time, 0.1 % Congo red solution is fl ooded on plates. The clear zone around the colony is a sign of cellulolytic activity (Aunpad and Panbangred 2003 ;Rohban et al 2009 ;Chen and Liu 2013 ). In Fig.…”

Section: Cellulasesmentioning

confidence: 94%

“…A halophilic chitinolytic bacterium, S. costicola strain 5SM-1 was isolated from a salted mud in Thailand. The chitinase gene ( chiC ) from this bacterium was cloned and the recombinant enzyme (ChiC) was produced by E. coli with the molecular mass of 92 kDa (Aunpad and Panbangred 2003 ).…”

Section: Chitinasesmentioning

confidence: 99%

Hydrolytic Enzymes in Halophilic Bacteria, Properties and Biotechnological Potential

Amoozegar

Siroosi

2015

Sustainable Development and Biodiversity

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Halophilic bacteria are one of the most important extremophilic microorganisms that can be found in saline or hypersaline environments which are widely distributed around the world. They are not only adapted to live in saline environments in where other organisms are not able to thrive and grow, but also subjected to other kinds of extreme conditions, like high pH values, high or low temperature, low oxygen availability, pressure, and toxic metals. Because of these abilities, biodiversity and biotechnological applications of halophiles have been studied since then these were introduced to microbiologists. Among different applications of halophilic bacteria, enzymes, especially hydrolases, always have received the most attraction in recent years. Enclosed is a summary review on biology of halophilic bacteria and their general applications with a closer look at the hydrolases produced by these microorganisms (haloenzymes).

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Cloning and Characterization of the Constitutively Expressed Chitinase C Gene from a Marine Bacterium, Salinivibrio costicola Strain 5SM-1

Cited by 9 publications

References 31 publications

Production and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26

Production and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26

In vivo and in vitro evaluation of antifungal activities from a halotolerant Bacillus subtilis strain J9

Hydrolytic Enzymes in Halophilic Bacteria, Properties and Biotechnological Potential

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