Cloning and characterization of the astaxanthin biosynthesis gene cluster from the marine bacterium Paracoccus haeundaensis

Lee, Jae Hyung; Kim, Young Tae

doi:10.1016/j.gene.2005.11.007

Cited by 43 publications

(27 citation statements)

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“…The biosynthesis of astaxanthin is limited to a few organisms such as the microalgae H. pluvialis [ 93 ], some marine bacteria such as Paracoccus haeundaensis [ 94 ] and Brevudimonas sp. [ 95 ], the basidiomycete yeast X. dendrorhous [ 92 ] and the plant Adonis , which accumulates astaxanthin in the petals of the fl ower [ 96 ].…”

Section: Astaxanthin Biosynthesis In X Dendrorhous and In Other Orgamentioning

confidence: 99%

Astaxanthin and Related Xanthophylls

2014

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Section: Astaxanthin Biosynthesis In X Dendrorhous and In Other Orgamentioning

confidence: 99%

Astaxanthin and Related Xanthophylls

2014

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“…and Brevundimonas sp. (Lee and Kim 2006;Misawa et al 1995a;1995b;Nishida et al 2005;Tao et al 2006a). Their homologous genes have also been isolated from H. pluvialis (Kajiwara et al 1995;Linden 1999;Lotan and Hirschberg 1995).…”

Section: Genes Mediating Biosynthesis Of Astaxanthin From B B-carotenementioning

confidence: 98%

Pathway engineering of plants toward astaxanthin production

Misawa

2009

Plant Biotechnology

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Astaxanthin responsible for the red color of red fish and crustacean has beneficial effects on human health as well as its utility as a pigmentation source in aquaculture. Astaxanthin biosynthetic pathway from b-carotene needs two enzymes, a carotenoid 4,4Ј-ketolase (oxygenase) and a carotenoid 3,3Ј-hydroxylase. b-Carotene is usually one of dominant carotenoids in higher plants, which lack the former enzyme. This mini-review elucidates in the earlier section the catalytic functions of a series of the ketolase and hydroxylase enzymes that have been isolated up to now. For the last ten years, pathway engineering approaches of plants including staple crops toward astaxanthin production have been undertaken by introducing and expressing in the plants a ketolase gene sometimes along with a hydroxylase gene. Several successful results have been achieved to produce large amounts of astaxanthin together with other ketocarotenoids in plant tissues such as carrot roots and tobacco leaves.

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“…In addition, we reported the structural and functional analysis of genes encoding the astaxanthin biosynthetic enzymes; GGPP synthase (CrtE), phytoene synthase (CrtB), phytoene desaturase (CrtI), lycopene cyclase (CrtY), ß-carotene hydroxylase (CrtZ), and ß-carotene ketolase (CrtW) [15]. The individual gene of the carotenoid biosynthesis gene cluster was functionally expressed in E. coli and each gene product was purified to homogeneity.…”

Section: Mbic1143 [22] and Paracoccus Haeundaensismentioning

confidence: 99%

“…2), was transformed into E. coli BL21(DE3) codon plus. In a previous study, we constructed pCR-XL-TOPO-Crt-full plasmid carrying the full-length astaxanthin biosynthesis gene cluster [15]. The pCR-XL-TOPO-Crt-full plasmid and pACYC184-lytB, pACYC184-idi, and pACYC184-ispA plasmid, respectively were transformed into E. coli BL21(DE3) Codon Plus RIL.…”

Section: Namementioning

confidence: 99%

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Enhanced Production of Astaxanthin by Metabolic Engineered Isoprenoid Pathway in Escherichia coli

Lee¹,

Seo²,

Kim³

2008

Journal of Life Science

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The goal of this study is to increase production of astaxanthin in recombinant Escherichia coli by engineered isoprenoid pathway. We have previously reported structural and functional analysis of the astaxanthin biosynthesis genes from a marine bacterium, Paracoccus haeundaensis. The carotenoid biosynthesis gene cluster involved in astaxanthin production contained six carotenogenic genes (crtW, crtZ, crtY, crtI, crtB, and crtE genes) and recombinant E. coli harboring six carotenogenic genes from P. haeundaensis produced 400 μg/g dry cell weight (DCW) of astaxanthin. In order to increase production of astaxanthin in recombinant E. coli, we have cloned 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (lytB), farnesyl diphosphate (FPP) synthase (ispA), and isopentenyl (IPP) diphossphate isomerase (idi) in the isoprenoid pathway from E. coli and coexpressed these genes in recombinant E. coli harboring the astaxanthin biosynthesis genes. This engineered E. coli strain containing both isoprenoid pathway gene and astaxanthin biosynthesis gene cluster produced 1,200 μg/g DCW of astaxanthin, resulting 3-fold increased production of astaxanthin. [14]. Even though synthetically produced astaxanthin has some drawbacks to apply commercial application, it is used for both direct and indirect food additives and coloring because naturally purified astaxanthin is very limited. In cosmetology and pharmacology, it is most demanding pigments for use as a dermal photoprotector. Astaxanthin is a pigment of high economic value and various study were carried out to increased the production of astaxanthin with the application of metabolic pathway engineering [1,18,25,28,31]. MBIC1143 [22], and Paracoccus haeundaensisIn a previous study, we isolated and characterized a marine bacterium, Paracoccus haeundaensis, which produces astaxanthin [14]. In addition, we reported the structural and functional analysis of genes encoding the astaxanthin biosynthetic enzymes; GGPP synthase (CrtE), phytoene synthase (CrtB), phytoene desaturase (CrtI), lycopene cyclase (CrtY), ß-carotene hydroxylase (CrtZ), and ß-carotene ketolase (CrtW) [15]. The individual gene of the carotenoid biosynthesis gene cluster was functionally expressed in E. coli and each gene product was purified to homogeneity. Their molecular characteristics, including enzymatic activities, were reported [16]. Furthermore, we have reported the pathways and the functions of the astaxanthin biosynthesis genes through chromatographic and spectroscopic analyses of the pigments accumulated in E. coli carrying plasmids constructed by various combinations of the carotenoid biosynthesis genes from the P. haeundaensis [17]. The astaxanthin biosynthesis pathway is summarized in Fig. 1.Enhanced production of astaxanthin in engineered microbial hosts requires optimization of the available precursors pool of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), and balancing the expression -Review -

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Cloning and characterization of the astaxanthin biosynthesis gene cluster from the marine bacterium Paracoccus haeundaensis

Cited by 43 publications

References 34 publications

Astaxanthin and Related Xanthophylls

Astaxanthin and Related Xanthophylls

Pathway engineering of plants toward astaxanthin production

Enhanced Production of Astaxanthin by Metabolic Engineered Isoprenoid Pathway in Escherichia coli

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