Cloning and characterization of the dopamine like receptor in the oyster Crassostrea angulata: Expression during the ovarian cycle

Yang, Bingye; Ni, Jianbin; Zeng, Zhen; Shi, Bo; Wang, You; Ke, Caihuan

doi:10.1016/j.cbpb.2012.12.006

Cited by 14 publications

(16 citation statements)

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“…The dopamine ligandreceptor system plays a part in the regulation of allocation of energy, growth, reproduction and defense (Lacoste et al, 2001a,b). The studied EST is 100% identical to the dopamine receptor d1 identified in Crassostrea angulata with a suggested important regulatory role in the early ovarian development and maturation stage (Yang et al, 2013). The relationship between reproductive effort and survival was first investigated from a quantitative genetics viewpoint (Ernande et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas

Normand

Quillien

et al. 2014

Marine Genomics

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Pacific oyster Crassostrea gigas suffers from chronic or sporadic mortality outbreaks worldwide, resulting from infectious diseases and/or physiological disorders triggered by environmental factors. Since 2008, ostreid herpesvirus OsHV-1 μVar has been identified as the main agent responsible for mass mortality of juvenile oysters in Europe. Previous studies of genome-wide expression profiling have provided candidate genes that potentially contribute to genetically-based resistance to summer mortality. To assess their value in determining resistance to the juvenile mass mortality that has occurred in France since 2008, we analyzed the expression of 17 candidate genes in an experimental infection by OsHV-1 μVar, and in an in vivo field experiment. Individual quantification of mRNA levels of 10 out of the 17 targeted genes revealed significant variation, of which 7 genes were showed differences between conditions that created significant differences in mortality, and 6 depended on the number of OsHV-1 genome copies individually quantified in mantle tissue. Complex SOD metalloenzymes known to be part of the antioxidant defense strategies may at least partly determine susceptibility or resistance to OsHV-1-associated mortality. Furthermore, inhibitor 2 of NF-κB, termed CgIκB2, exhibited highly significant variation of mRNA levels depending on OsHV-1 load in both experiments, suggesting its implication in the antiviral immune response of C. gigas. Our results suggest that CgIκB2 expression would make a good starting point for further functional research and that it could be used in marker-assisted selection.

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Section: Discussionmentioning

confidence: 99%

Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas

Normand

Quillien

et al. 2014

Marine Genomics

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“…These results suggested that CgGPR1 is also likely to play roles in adult organs. The expression pattern of CgGPR1 in the organs differed from those of other reported oyster biogenic amine receptors including the adrenergic receptor AR cga [48] and the DA receptor Ca-DA1R [26], which both exhibited highest expression levels in the labial palps. The different expression patterns between CgGPR1 and other tyrosine derivative receptors suggested the functional differentiation of GPCRs for different biogenic amine receptors in oysters.…”

Section: Resultsmentioning

confidence: 63%

“…To acquire the complete cDNA sequence of CgGPR1 , 5′ and 3′ rapid amplification of cDNA ends (RACE) procedures were performed as previously reported [26]. Primer sequences used in the amplification of CgGPR1 are listed in the supplementary material (S1 Table).…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization and Functional Analysis of a Putative Octopamine/Tyramine Receptor during the Developmental Stages of the Pacific Oyster, Crassostrea gigas

Huang

et al. 2016

PLoS ONE

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Octopamine (OA) and its precursor, tyramine (TA), participate in invertebrate development such as growth, maturation, and reproduction by activating their corresponding G protein-coupled receptors (GPCRs). Although OA was first discovered in mollusks (octopus), subsequent studies on OA, TA and related receptors have primarily been conducted in Ecdysozoa, especially in insects. Accordingly, only limited reports on OA/TA receptors in mollusks are available and their physiological roles remain unclear. Here, a full-length cDNA encoding a putative 524 amino acid OA/TA receptor (CgGPR1) was isolated from the Pacific oyster Crassostrea gigas. CgGPR1 was most closely related to the Lymnaea stagnalis OA receptor OAR2 in sequence. Phylogenetic analysis showed that CgGPR1 belongs to a poorly studied subfamily of invertebrate OA/TA receptors. The spatio-temporal expression of CgGPR1 in C. gigas larvae was examined by quantitative real-time PCR and Western blot analysis. CgGPR1 was expressed during all developmental stages of C. gigas with higher levels at mid-developmental stages, indicating its potential role in embryogenesis and tissue differentiation. Immunoreactive fluorescence of CgGPR1 was mainly observed in the velum, foot, gill and mantle of C. gigas larvae. CgGPR1 transcripts were detected in all the tested organs of adult C. gigas, with highest level in the mantle. Pharmacological analysis showed that cAMP and Ca2+ concentrations remained unchanged in HEK293 cells expressing CgGPR1 upon addition of OA, TA or related amines, suggesting that CgGPR1 modulates other unknown molecules rather than cAMP and Ca2+. Our study sheds light on CgGPR1 function in oysters.

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“…In order to clone the full sequence of the chitinase-coding gene, 5′-RACE and 3′-RACE tests were conducted separately using the Takara 5′-full RACE and 3′-full RACE cDNA Amplification Kit (Takara, Dalian, China) according to the manufacturer's instructions. The processes involved were described in detail previously, except for the primers [ 21 ]. The specific primers related to Ca -chit were designed based on the nucleotide sequence of chitinase cDNA fragments, which were obtained from the C .…”

Section: Methodsmentioning

confidence: 99%

“…There are three oysters in each group. The process of quantitative RT-PCR was described in detail previously [ 21 ]. The RT- qPCR reactions were conducted on ABI7500FAST.…”

Section: Methodsmentioning

confidence: 99%

Molecular Analysis of Atypical Family 18 Chitinase from Fujian Oyster Crassostrea angulata and Its Physiological Role in the Digestive System

Yang

Zhang

et al. 2015

PLoS ONE

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Chitinolytic enzymes have an important physiological significance in immune and digestive systems in plants and animals, but chitinase has not been identified as having a role in the digestive system in molluscan. In our study, a novel chitinase homologue, named Ca-Chit, has been cloned and characterized as the oyster Crassostrea angulate. The 3998bp full-length cDNA of Ca-Chit consisted of 23bp 5-UTR, 3288 ORF and 688bp 3-UTR. The deduced amino acids sequence shares homologue with the chitinase of family 18. The molecular weight of the protein was predicted to be 119.389 kDa, with a pI of 6.74. The Ca-Chit protein was a modular enzyme composed of a glycosyl hydrolase family 18 domain, threonine-rich region profile and a putative membrane anchor domain. Gene expression profiles monitored by quantitative RT-PCR in different adult tissues showed that the mRNA of Ca-Chit expressed markedly higher visceral mass than any other tissues. The results of the whole mount in-situ hybridization displayed that Ca-Chit starts to express the visceral mass of D-veliger larvae and then the digestive gland forms a crystalline structure during larval development. Furthermore, the adult oysters challenged by starvation indicated that the Ca-Chit expression would be regulated by feed. All the observations made suggest that Ca-Chit plays an important role in the digestive system of the oyster, Crassostrea angulate.

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Cloning and characterization of the dopamine like receptor in the oyster Crassostrea angulata: Expression during the ovarian cycle

Cited by 14 publications

References 50 publications

Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas

Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas

Molecular Characterization and Functional Analysis of a Putative Octopamine/Tyramine Receptor during the Developmental Stages of the Pacific Oyster, Crassostrea gigas

Molecular Analysis of Atypical Family 18 Chitinase from Fujian Oyster Crassostrea angulata and Its Physiological Role in the Digestive System

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