Cloning and characterization of the Mx1, Mx2 and Mx3 promoters from gilthead seabream (Sparus aurata)

González-Mariscal, J.A.; Gallardo-Gálvez, J.B.; Méndez, T.; Álvarez, M. Carmen; Béjar, Julia

doi:10.1016/j.fsi.2014.03.031

Cited by 16 publications

(14 citation statements)

References 56 publications

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“…During the last years several biochemical, molecular and cellular tools have been developed from the gilthead seabream (Fonseca et al, 2011;Marques et al, 2007;Pombinho et al, 2004;Tiago et al, 2014). This together with the increasing availability of gene and transcripts in public sequence databases validated the gilthead seabream as a suitable model to better understand mechanisms of gene regulation, and this was further confirmed by several genetic and functional studies A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT (Conceição et al, 2008;Ferraresso et al, 2008;González-Mariscal et al, 2014;Rafael et al, 2006;Rosa et al, 2014).…”

Section: Introductionmentioning

confidence: 89%

Transcriptional regulation of gilthead seabream bone morphogenetic protein (BMP) 2 gene by bone- and cartilage-related transcription factors

Marques

Cancela

Laizé

2016

Gene

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Bone morphogenetic protein (BMP) 2 belongs to the transforming growth factor β (TGFβ) superfamily of cytokines and growth factors. While it plays important roles in embryo morphogenesis and organogenesis, BMP2 is also critical to bone and cartilage formation. Protein structure and function have been remarkably conserved throughout evolution and BMP2 transcription has been proposed to be tightly regulated, although few data is available. In this work we report the cloning and functional analysis of gilthead seabream BMP2 promoter. As in other vertebrates, seabream BMP2 gene has a 5′ non-coding exon, a feature already present in DPP gene, the fruit fly ortholog of vertebrate BMP2 gene, and maintained throughout evolution. In silico analysis of seabream BMP2 promoter revealed several binding sites for bone and cartilage related transcription factors (TFs) and their functionality was evaluated using promoter-luciferase constructions and TF-expressing vectors. Runt-related transcription factor 3 (RUNX3) was shown to negatively regulate BMP2 transcription and combination with the core binding factor β (CBFβ) further reduced transcriptional activity of the promoter. Although to a lesser extent, myocyte enhancer factor 2C (MEF2C) had also a negative effect on the regulation of BMP2 gene transcription, when associated with SRY (sex determining region Y)-box 9 (SOX9b). Finally, v-ets avian erythroblastosis virus E26 oncogene homolog 1 (ETS1) was able to slightly enhance BMP2 transcription. Data reported here provides new insights toward the better understanding of the transcriptional regulation of BMP2 gene in a bone and cartilage context.

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Section: Introductionmentioning

confidence: 89%

Transcriptional regulation of gilthead seabream bone morphogenetic protein (BMP) 2 gene by bone- and cartilage-related transcription factors

Marques

Cancela

Laizé

2016

Gene

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“…Vertebrate Mx were compared, and similarities grouped them into fish mx, avian mx, human mx2-like, and human mx1-like [39]. Diverse mx proteins are found in fish [40].…”

Section: Primary Responsementioning

confidence: 99%

Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient <i>Rag1<sup>-/-</sup> </i> Mutant Zebrafish

Krishnavajhala¹,

Muire²,

Hanson³

et al. 2017

IJI

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Abstract:To elucidate the basis of protective immunity in T and B cell deficient rag1 -/-mutant zebrafish, we conducted microarray analysis of 15,617 genes from rag1 -/-mutant zebrafish 48 hours after a primary response and 48 hours after a secondary response. Following primary exposure, the highest fold expression differences (3.8 to 4.95) were genes for serum amyloid A, chemokine CCL-C5a (CCL-19a), signal transducer and activator of transcription (STAT) 1b, interferon regulatory factor 11, and myxovirus resistance A. Strong induction of these genes demonstrated that primary immune responses and innate immune cells were not impaired in T and B cell deficient mutant zebrafish. Following bacterial re-exposure, the highest fold expression differences (2 to 3 fold) were in chemokine CCL-C5a (CCL-19a), myomegalin, bone morphogenetic protein 4, and relaxin 3a. These genes are involved in the immune response and cell proliferation. Genes for cell receptor activation and signal transduction, cell proliferation and cytotoxic functions were also up-regulated. These findings suggest receptor activation and expansion of a cell population. Increased ifnγ expression at 48 hpi was associated with both primary and secondary immune responses.

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“…As a first approach in assessing the regulation of seabream Mx genes expression, the structure of Mx1, Mx2 and Mx3 promoters (pMx1, pMx2 and pMx3) was disclosed (Fig. 1S), and their response to poly I:C in RTG-2 cells was characterized (González-Mariscal et al, 2014). The results revealed a clear induction of all of them, though differences in the kinetics and magnitude of their response were reported.…”

Section: Figmentioning

confidence: 99%

“…and Mx3 promoters, respectively) (González-Mariscal et al, 2014). Two independent transfections were carried out for each virus.…”

Section: Transient Cell Transfectionsmentioning

confidence: 99%

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Gilthead seabream (Sparus aurata) Mx gene promoters respond differentially to IPNV and VHSV infections in RTG-2 cells

González-Mariscal

Fernández-Trujillo

Alonso

et al. 2016

Veterinary Immunology and Immunopathology

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Cloning and characterization of the Mx1, Mx2 and Mx3 promoters from gilthead seabream (Sparus aurata)

Cited by 16 publications

References 56 publications

Transcriptional regulation of gilthead seabream bone morphogenetic protein (BMP) 2 gene by bone- and cartilage-related transcription factors

Transcriptional regulation of gilthead seabream bone morphogenetic protein (BMP) 2 gene by bone- and cartilage-related transcription factors

Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient <i>Rag1<sup>-/-</sup> </i> Mutant Zebrafish

Gilthead seabream (Sparus aurata) Mx gene promoters respond differentially to IPNV and VHSV infections in RTG-2 cells

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Cloning and characterization of the Mx1, Mx2 and Mx3 promoters from gilthead seabream (Sparus aurata)

Cited by 16 publications

References 56 publications

Transcriptional regulation of gilthead seabream bone morphogenetic protein (BMP) 2 gene by bone- and cartilage-related transcription factors

Transcriptional regulation of gilthead seabream bone morphogenetic protein (BMP) 2 gene by bone- and cartilage-related transcription factors

Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient &lt;i&gt;Rag1&lt;sup&gt;-/-&lt;/sup&gt; &lt;/i&gt; Mutant Zebrafish

Gilthead seabream (Sparus aurata) Mx gene promoters respond differentially to IPNV and VHSV infections in RTG-2 cells

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Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient <i>Rag1<sup>-/-</sup> </i> Mutant Zebrafish