Gilthead seabream (Sparus aurata) Mx gene promoters respond differentially to IPNV and VHSV infections in RTG-2 cells

González-Mariscal, J.A.; Fernández-Trujillo, M.A.; Alonso, M. Carmen; García-Rosado, Esther; Álvarez, M. Carmen; Béjar, Julia

doi:10.1016/j.vetimm.2016.02.006

Cited by 5 publications

(2 citation statements)

References 47 publications

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“…RTG-2 cells are a validated experimental system for the functional characterization of virus-host interactions (Tafalla et. al., 2007;González-Mariscal et al, 2016). This suggests that a higher protection against a viral infection could be achieved in the presence of this secretagogue.…”

Section: Introductionmentioning

confidence: 97%

Growth hormone secretagogue peptide A233 upregulates Mx expression in teleost fish in vitro and in vivo

Martı́nez¹,

Fernández-Trujillo

Hernández³

et al. 2022

Arch Virol

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Aquaculture constitutes an alternative source for food production and contributes to the reduction of indiscriminate catch of aquatic organisms from their natural environment (Diaz & Neira, 2005;Beveridge et al., 2013). However, high mortality during larval state remains a challenge in this sector, mainly because of factors like diets and diseases induced by pathogens (Helvik et al., 2009;Stentiford et al., 2017). Therefore, growth and health management is a key strategy for sustainable aquaculture (Martinez et al., 2016a).

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Section: Introductionmentioning

confidence: 97%

Growth hormone secretagogue peptide A233 upregulates Mx expression in teleost fish in vitro and in vivo

Martı́nez¹,

Fernández-Trujillo

Hernández³

et al. 2022

Arch Virol

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“…Furthermore, different isoforms of Mx genes are found in many species. Two isoforms of Mx genes have been identified in humans [10] and mice [11], seven in zebrafish [12], nine in rainbow trout-Oncorhynchus mykiss, and four in European eel-Anguilla anguilla [13]. Currently, there are a few reports on Mx in amphibians.…”

Section: Introductionmentioning

confidence: 99%

Characterization, Expression Pattern and Antiviral Activities of Mx Gene in Chinese Giant Salamander, Andrias davidianus

Liu

Zhou

et al. 2020

IJMS

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Mx, Myxovirus resistance is an important interferon-stimulated protein that mediates antiviral responses. In this study, the expression and activities of Chinese giant salamander, Andrias davidianus Mx gene, AdMx, were investigated. The AdMx cDNA sequence contains an open reading frame (ORF) of 2112 nucleotides, encoding a putative protein of 703 aa. Meanwhile, AdMx possesses the conserved tripartite GTP binding motif and a dynamin family signature. qRT-PCR analysis revealed a broad expression of AdMx in vivo, with the highest expression levels in brain, kidney and spleen. The AdMx expression level in kidney, spleen and muscle significantly increased at 6 h after Chinese giant salamander iridovirus (GSIV) infection and peaked at 48 h, while that in muscle cell line (GSM) was not noticeably up-regulated until 72 h post infection. Additionally, a plasmid expressing AdMx was constructed and transfected into the Chinese giant salamander GSM cells. The virus load and gene copies in AdMx over-expressed cells were significantly reduced compared with those in the control cells. Moreover, compared to the control cells, a lower level of virus major capsid protein (MCP) synthesis in AdMx over-expressed cells was confirmed by Western blot. These results collectively suggest that Mx plays an important antiviral role in the immune responses against GSIV in Chinese giant salamander.

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Comparative analysis of marine and freshwater viral haemorrhagic septicaemia virus (VHSV) isolates antagonistic activity

Gémez-Mata

Álvarez-Torres

García-Rosado

et al. 2020

Comparative Immunology, Microbiology and Infectious Diseases

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Gilthead seabream (Sparus aurata) Mx gene promoters respond differentially to IPNV and VHSV infections in RTG-2 cells

Cited by 5 publications

References 47 publications

Growth hormone secretagogue peptide A233 upregulates Mx expression in teleost fish in vitro and in vivo

Growth hormone secretagogue peptide A233 upregulates Mx expression in teleost fish in vitro and in vivo

Characterization, Expression Pattern and Antiviral Activities of Mx Gene in Chinese Giant Salamander, Andrias davidianus

Comparative analysis of marine and freshwater viral haemorrhagic septicaemia virus (VHSV) isolates antagonistic activity

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