Cloning and characterization of Arenicola marina peroxiredoxin 6, an annelid two-cysteine peroxiredoxin highly homologous to mammalian one-cysteine peroxiredoxins☆

A novel 1-Cys thioredoxin peroxidase gene in Apis cerana cerana: characterization of AccTpx4 and its role in oxidative stresses

“…Furthermore, the purified recombinant AccTpx4 protein could efficiently degrade H 2 O 2 in the presence of DTT (Fig. 7b), similar to Arenicola marina PRDX6 (Loumaye et al 2008) and AbTPx2 (Pushpamali et al 2008) but different from the AccTpx4 induced by H 2 O 2 (Fig. 5h).…”

“…The signal of the binding reactions was visualized with HRP substrates antioxidative response , signal transduction (Jarvis et al 2012;Wood et al 2003a), and the regulation of phospholipid metabolism (Chen et al 2000;Manevich and Fisher 2005). 1-Cys Prx, also classified as Prx6 (Mizohata et al 2005;Nelson et al 2011), is a special type of peroxidase in which the resolving Cys is missing and the sulfenic acid of the peroxidatic Cys can be reduced by a heterologous thiolcontaining reductant (Loumaye et al 2008). In this study, we isolated a 1-Cys Prx (AccTpx4) from A. cerana cerana.…”

Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus

“…To date, a number of Prx6 have been identified in marine species such as Antarctic bivalve Laternula elliptica [16], Chinese shrimp Fenneropenaeus chinensis [17], disk abalone Haliotis discus discus [18], Pacific oyster Crassostrea gigas [19], and Arenicola marina [20]. However, only four fish Prx6 have been reported at sequence level, which are Prx6 from Salmo salar, Oncorhynchus mykiss, Ictalurus punctatus, and Danio rerio (GenBank accession nos: ACI67571, NP_001158604, ABG77029, and NP_957099, respectively).…”

Molecular cloning, characterization and expression analysis of peroxiredoxin 6 from disk abalone Haliotis discus discus and the antioxidant activity of its recombinant protein

“…Interestingly, a catalytic triad was in the positions of Cys44-His36-Arg127 in abalone, which is similar to E. sinensis. Abalone's peroxidatic Cys, its Ser residue, which is responsible for phospholipase A2 activity, and His and Arg, which form this catalytic triad for peroxidase activity, were homologous to the peroxidatic Cys45, Ser32, His37 and Arg128 in the annelid A. marina PrdxVI [31]. The GDSWG motif, which is responsible for PLA2 activity, was modified to 27 GGSWA 33 in abalone's amino acid position 28.…”

“…Recently, PrxVI (also referred to as ORF6 and AOP2) has been cloned from several vertebrates and invertebrates, including human (Homo sapiens) [25,26], rat (Rattus norvegicus) [27], cattle (Bos taurus) [13], chicken (Gallus gallus) [28], channel catfish (Ictalurus punctatus) [29] Chinese mitten crab (Eriocheir sinensis) [30] and annelid worm (Arenicola marina) [31], and their cDNA sequences have been characterized. Recombinant proteins have been over-expressed, purified and characterized for their functional activities in some of the organisms [15,17,18,22,27,32].…”