Cloning and Characterization of Aerobactin Biosynthesis Genes of the Biological Control Agent
            <i>Enterobacter cloacae</i>

Loper, Joyce E.; Ishimaru, Carol A.; Carnegie, Susan R.; Vanavichit, Apichart

doi:10.1128/aem.59.12.4189-4197.1993

Cited by 18 publications

(1 citation statement)

References 52 publications

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“…The ability to produce an antibiotic-like substance was found in strain E. aerogenes B8 (39). Strains of E. cloacae and E. agglomerans were found to produce hydroxamate siderophores (2,16) and different volatile and nonvolatile antifungal metabolites (11,37). Competition for nutrients and rhizosphere colonization ability were considered possible mechanisms of antifungal activity in E. cloacae (40) and E. agglomerans (10), respectively.…”

mentioning

confidence: 99%

Chitinolytic Enterobacter agglomerans Antagonistic to Fungal Plant Pathogens

Chernin¹,

Ismailov²,

Haran³

et al. 1995

Appl Environ Microbiol

317

115

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Three Enterobacter agglomerans strains which produce and excrete proteins with chitinolytic activity were found while screening soil-borne bacteria antagonistic to fungal plant pathogens. The chitinolytic activity was induced when the strains were grown in the presence of colloidal chitin as the sole carbon source. It was quantitated by using assays with chromogenic p-nitrophenyl analogs of disaccharide, trisaccharide, and tetrasaccharide derivatives of N-acetylglucosamine. A set of three fluorescent substrates with a 4-methylumbelliferyl group linked by ␤-1,4 linkage to N-acetylglucosamine mono-or oligosaccharides were used to identify the chitinolytic activities of proteins which had been renatured following their separation by electrophoresis. This study provides the most complete evidence for the presence of a complex of chitinolytic enzymes in Enterobacter strains. Four enzymes were detected: two N-acetyl-␤-D-glucosaminidases of 89 and 67 kDa, an endochitinase with an apparent molecular mass of 59 kDa, and a chitobiosidase of 50 kDa. The biocontrol ability of the chitinolytic strains was demonstrated under greenhouse conditions. The bacteria decreased the incidence of disease caused by Rhizoctonia solani in cotton by 64 to 86%. Two Tn5 mutants of one of the isolates, which were deficient in chitinolytic activity, were unable to protect plants against the disease.

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mentioning

confidence: 99%

Chitinolytic Enterobacter agglomerans Antagonistic to Fungal Plant Pathogens

Chernin¹,

Ismailov²,

Haran³

et al. 1995

Appl Environ Microbiol

317

115

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A Widely Distributed Bacterial Pathway for Siderophore Biosynthesis Independent of Nonribosomal Peptide Synthetases

2005

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Discovery and Characterization of New Hydroxamate Siderophores, Baumannoferrin A and B, produced by Acinetobacter baumannii

et al. 2015

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Acinetobacter baumannii AYE does not produce acinetobactin but grows under iron limitation. Accordingly, analyses of AYE iron-restricted culture supernatants resulted in the isolation of two fractions, which contained only hydroxamates and showed siderophore activity. Structural analyses identified baumannoferrin A and baumannoferrin B, which differ only by a double bond. These siderophores are composed of citrate, 1,3-diaminopropane, 2,4-diaminobutyrate, decenoic acid, and α-ketoglutarate. Analysis of the AYE genome showed the presence of a 12-gene cluster coding for proteins similar to those involved in the production and utilization of the hydroxamate siderophores acinetoferrin and achromobactin. As A. baumannii AYE does not produce acinetobactin and harbors only one gene cluster encoding the production and utilization of a siderophore, this strain's growth under iron limitation depends on baumannoferrin, a novel hydroxamate that could play a role in its virulence.

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Cloning and Characterization of Aerobactin Biosynthesis Genes of the Biological Control Agent Enterobacter cloacae

Cited by 18 publications

References 52 publications

Chitinolytic Enterobacter agglomerans Antagonistic to Fungal Plant Pathogens

Chitinolytic Enterobacter agglomerans Antagonistic to Fungal Plant Pathogens

A Widely Distributed Bacterial Pathway for Siderophore Biosynthesis Independent of Nonribosomal Peptide Synthetases

Discovery and Characterization of New Hydroxamate Siderophores, Baumannoferrin A and B, produced by Acinetobacter baumannii

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