Cloning and characterization of ACC oxidase genes from tulip

Momonoi, Kazumi; Shoji, K.; Yoshida, Kumi

doi:10.5511/plantbiotechnology.24.241

Cited by 11 publications

(4 citation statements)

References 18 publications

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“…Liu et al (1997) reported a similar expression pattern of ACC oxidase 2 in 4-day-old sunflower seedlings. In tulip, of the five ACO genes identified, only TgACO3 was expressed in leaves and only TgACO5 was expressed in stems; transcripts of the other three genes were identified in flowers (Momonoi et al, 2007). The higher PnACO3 transcript level in cotyledons supports suggestions by Yang and Hoffman (1984) and Nie et al (2002) that ACC is synthesized mainly in roots and then transported to higher parts of the plant, where it is converted to ethylene.…”

Section: Resultssupporting

confidence: 71%

Involvement of the IAA-Regulated ACC Oxidase Gene PnACO3 in Pharbitis Nil Flower Inhibition

Wilmowicz¹,

Frankowski²,

Kućko³

et al. 2014

Acta Biologica Cracoviensia S. Botanica

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The study examined the influence of light and auxin on the transcription level of PnACO3, a gene involved in ethylene production, in relation to the inhibitory effect of ethylene on flower induction in the short-day plant Pharbitis nil (=Ipomoea nil). Exogenous auxin was shown to increase the level of PnACO3 mRNA, with the effect depending on the experimental conditions. Light did not affect the level of PnACO3 mRNA. Applying auxin to seedling cotyledons at the beginning of inductive night boosted PnACO3 transcriptional activity even threefold during the next few hours, supporting our previous suggestion that the inhibitory effect of auxin on P. nil flowering results from its stimulatory effect on ethylene production. K Ke ey y w wo or rd ds s: : ACC oxidase, auxin, ethylene, flowering, Pharbitis nil.

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Section: Resultssupporting

confidence: 71%

Involvement of the IAA-Regulated ACC Oxidase Gene PnACO3 in Pharbitis Nil Flower Inhibition

Wilmowicz¹,

Frankowski²,

Kućko³

et al. 2014

Acta Biologica Cracoviensia S. Botanica

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“…MD-ACO1 is restricted to fruit tissues, with optimal expression in ripening fruit, MD-ACO2 expression occurs more predominantly in younger fruit tissue, with some expression in young leaf tissue, while MD-ACO3 is expressed predominantly in young and mature leaf tissue, with less expression in young fruit tissue and least expression in ripening fruit. Also Momonoi et al (2007) observed a differential regulation of five ACO paralogs in tulip among the vegetative tissues and during flower senescence.…”

Section: Expression Activity Of Aco Genesmentioning

confidence: 87%

“…During vegetative development, differential ACO gene expression has been observed in mung bean (Kim and Yang 1994), tomato (Barry et al 1996;Moeder et al 2002), sunflower (Liu et al 1997), potato (Nie et al 2002), and tulip (Momonoi et al 2007) tissues.…”

Section: Vegetative Phase Of Developmentmentioning

confidence: 99%

Regulation of ethylene biosynthesis at the level of 1-aminocyclopropane-1-carboxylate oxidase (ACO) gene

2012

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Ethylene, a gaseous plant hormone regulates essentially all physiological processes during the plant's life cycle. The practical implications of ethylene biosynthesis regulation for plant improvement have supported the continuous basic research on dissecting the structure of genes encoding ethylene biosynthetic enzymes, their differential expression patterns, and mechanisms underlying their transcriptional activity. ACC oxidase (ACO) is involved in the final step of ethylene production in plant tissues. In various plants several ACO isoforms have been isolated which are encoded by a multigenic family. There is a strong evidence that ACO gene expression is positively correlated to the ethylene production rates and its multiple isoforms are under development and environmental control. Thus, the regulation of ACO gene activity may act either as an additional or in several cases also as a main level for controlling ethylene biosynthesis in higher plants. This review summarizes in detail the knowledge about organization and gene structure, and transcriptional expression of ACO genes from different plant species. The perspectives of manipulating ACO gene as a method in biotechnological modification of ethylene synthesis are also discussed.

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“…mayoría relacionadas con la maduración del fruto. El alineamiento con otras seis secuencias de aminoácidos correspondientes a ACCo (Figura 5), mostró sólo dos de ocho regiones altamente conservadas entre todas las secuencias de ACCo (Momonoi et al, 2007). Sin embargo, en el dendrograma (Figura 6) se aprecia una identidad estrecha con otras secuencias de ACCo, en particular con una involucrada en la maduración del fruto de la guayaba 'Enana' (Agüero et al, 2003).…”

Section: Pc-pg2unclassified

ADNc RELACIONADOS CON LA MADURACIÓN DEL FRUTO DE GUAYABA (Psidium guajava L.). CARACTERIZACIÓN Y ANÁLISIS DE EXPRESIÓN

et al. 2013

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En este estudio se presentan los análisis bioinformáticos y de la expresión de cuatro clonas de ADNc que codifican para una poligalacturonasa (PG), para ácido 1 aminociclopropano-1-carboxílico oxidasa (ACCo), y para dos α-expansinas (α-Exp) en guayaba (Psidium guajava L.). Mediante RT-PCR se obtuvo un fragmento parcial de 301 pb (PgPG1) correspondiente a una PG que se expresa a partir de fruto maduro, uno de 320 pb (PgACO1) para una ACCo de fruto sobremaduro, y dos para α expansinas: uno de 466 pb (PgEXP2) de fruto sobremaduro y otro de 362 pb (PgEXP3) de pedúnculo. El análisis bioinformático de los ADNc mostró que codifican para proteínas putativas con una alta homología con proteínas relacionadas. La secuencia de aminoácidos de la proteína parcial PgPG1 contiene regiones características y conservadas de las PGs en plantas superiores y está relacionada con la maduración de frutos; PgACO1 mostró características presentes en todas las ACCo y está relacionada con la maduración; PgEXP2 y PgEXP3 contienen parte de los dos dominios presentes de las expansinas, y están agrupadas filogenéticamente con las α-expansinas. Los estudios de expresión mediante Dot Blot mostraron que el gen PgPG1 fue visible en todos los estadios de maduración del fruto, con mayor intensidad durante el estadio maduro; el gen PgACO1 fue visible en los cinco estadios de maduración del fruto y presentó su expresión más alta durante el estadio de transición, cuando comienza el cambio de color verde al amarillo (estos dos genes muestran comportamientos similares a los reportados en frutos climatéricos); en PgEXP2 la expresión génica se detectó en todos los tejidos, con un incremento a partir del estadio verde 2 al sobremaduro, similar al comportamiento reportado en frutos no climatéricos; para PgEXP3 la expresión fue visible en cuatro estadios de maduración del fruto y en pedúnculo, con mayor intensidad en el estadio maduro que en todos los demás.

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Cloning and characterization of ACC oxidase genes from tulip

Cited by 11 publications

References 18 publications

Involvement of the IAA-Regulated ACC Oxidase Gene PnACO3 in Pharbitis Nil Flower Inhibition

Involvement of the IAA-Regulated ACC Oxidase Gene PnACO3 in Pharbitis Nil Flower Inhibition

Regulation of ethylene biosynthesis at the level of 1-aminocyclopropane-1-carboxylate oxidase (ACO) gene

ADNc RELACIONADOS CON LA MADURACIÓN DEL FRUTO DE GUAYABA (Psidium guajava L.). CARACTERIZACIÓN Y ANÁLISIS DE EXPRESIÓN

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