Cloning and Characterization of a Stearoyl–Acyl Carrier Protein Desaturase Gene from Cinnamomum longepaniculatum

Luò, Tāo; Deng, Wu Yuan; Zeng, Jin; Zhang, Fu Li

doi:10.1007/s11105-008-0046-3

Cited by 25 publications

(21 citation statements)

References 11 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The plant stearoyl-ACP desaturase is the only soluble desaturase identified to date. All other desaturases identified in plants, algae, animals, and fungi are integral membrane proteins (Singh et al 2002;Luo et al 2009). There were seven prospective stearoyl-ACP desaturases in Arabidopsis while soybean had five homologous genes that consisted of three full-length and two partial ones.…”

Section: Phylogenetic Analysis Of Fatty Acid Desaturase Genes From Somentioning

confidence: 99%

Genome-Wide Analysis of Fatty Acid Desaturases in Soybean (Glycine max)

Chi¹,

Yang²,

et al. 2011

Plant Mol Biol Rep

View full text Add to dashboard Cite

Fatty acid desaturases can introduce double bonds into the hydrocarbon chains of fatty acids to produce unsaturated fatty acids. In the present study, 29 full-length desaturase genes were identified from soybean genome by a thorough annotation exercise. A comprehensive analysis was performed to characterize phylogeny, chromosomal locations, structures, conserved motifs, and expression patterns of those genes. The soybean genes were phylogenetically clustered into nine subfamilies with the Arabidopsis counterparts, FAB2, FAD2, FAD3, FAD5, FAD6, FAD7, FAD8, SLD1, and DES1. Twenty-nine desaturase genes were found to be distributed on at least 15 of the 20 soybean chromosomes. The gene structures and motif compositions were considerably conserved among the subfamilies. The majority of desaturase genes showed specific temporal and spatial expression patterns across different tissues and developmental stages based on microarray data analyses. The study may provide new insights into the origin and evolution of fatty acid biosynthesis pathways in higher plants. Additionally, the characterization of desaturases from soybean will lead to the identification of additional genes for genetic modification of plants to produce nutritionally important fatty acids.Keywords Glycine max . Genome . Fatty acid desaturase . Phylogenetic analysisΔ5 Desaturase DesC Cyanobacterial Δ9 desaturase DesA Cyanobacterial Δ12 desaturase DesB Cyanobacterial ω3 desaturase

show abstract

Section: Phylogenetic Analysis Of Fatty Acid Desaturase Genes From Somentioning

confidence: 99%

Genome-Wide Analysis of Fatty Acid Desaturases in Soybean (Glycine max)

Chi¹,

Yang²,

et al. 2011

Plant Mol Biol Rep

View full text Add to dashboard Cite

show abstract

“…Amplification was conducted by subjecting the samples to the following conditions: initial denaturation at 94°C for 6 min, followed by 30 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, with a final extension at 72°C for 7 min. The PCR products were then resolved by electrophoresis on 1% agarose gel, after which the fragment of interest was excised, purified using an agarose gel DNA fragment recovery kit (Tiangen Biotech Co. China), cloned into PMD-18 T vector (TaKaRa Biotech Co., Dalian, China), and sequenced (Luo et al 2009). …”

Section: Cloning and Sequencing Of Lhcsr Cdnamentioning

confidence: 99%

Characterization of the LhcSR Gene Under Light and Temperature Stress in the Green Alga Ulva linza

et al. 2011

View full text Add to dashboard Cite

As a green-tide-forming macroalga, Ulva linza is distributed worldwide and therefore subject to various environmental stresses. The LHCSR (also known as LI818 in green alga and LHCX in diatoms) protein is a stressrelated member of the LHC family that plays an important role in photo-protective mechanism, which has been only found in algae. In this study, we cloned full-length cDNA sequence encoding the LhcSR gene from U. linza and analyzed its expression in response to different temperature and illumination gradients. The results showed that high light (HL) could enhance expression of LhcSR and that the expression level peaked at 3 h under HL. Similarly, the expression of LhcSR could also be induced by low temperature (LT). However, the expression patterns of LhcSR were quite different in response to LT and HL treatment. Specifically, the maximum gene expression under LT was much higher (11.8-fold) than under HL (5.4-fold) when compared to the expression under normal conditions. The upregulated expression of LhcSR lasted for 12 h under LT, but 3 h under HL. These data suggest that the LhcSR gene is involved in photoprotection in U. linza, and the results suggest a stronger link to LT. In addition, the discrepancy in expression under HL and LT was consistent with the ecological features of this alga, which only thrives during the cold season (featured as LT and low light).

show abstract

“…Tasseva et al (2004) suggested that the expression of the SAD genes in plants will increase the cold tolerance in plants due to the increased desaturation of the fatty acids and thus a better membrane control of damage at the membrane level. Some SAD genes have also been cloned from several plant sources by different techniques (Luo et al, 2009;Knutzon et al, 1991;Los et al, 1998;Shah et al, 2000). Homology analysis and multiple sequence alignment analysis showed the SAD genes from different plants had high level of identity in amino acid sequence.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning of two novel stearoyl-acyl desaturase genes from winterness wheat

Guo

Ma³

et al. 2011

Genes Genom

View full text Add to dashboard Cite

Using RT-PCR and rapid amplification of cDNA ends, two new full-length cDNAs of SAD (TaSAD1 and TaSAD2) were obtained from a hardiest winter wheat cultivar (Mironovskaya808). Sequence comparison analysis showed that the deduced amino acid sequences of TaSAD1 and TaSAD2 had high similarity to those of other reported SAD proteins. They were also different each other by some substitutions, insertions and/or deletions involving single amino acid residues or motifs. Based on evolution analysis, it was clear that all SAD genes from Poaceae were closer than those from other genus such as Arabidopsis, Glycine, Triadica, Brassica, Sesamum and Bassia. All SAD genes clustered into two major groups in Poaceae. Meanwhile, TaSAD1 and TaSAD2 were clustered into different groups. The tertiary structure prediction indicated that both TaSAD1 and TaSAD2 proteins were a compact globular protein and their model structures almost were the same.

show abstract

Cloning and Characterization of a Stearoyl–Acyl Carrier Protein Desaturase Gene from Cinnamomum longepaniculatum

Cited by 25 publications

References 11 publications

Genome-Wide Analysis of Fatty Acid Desaturases in Soybean (Glycine max)

Genome-Wide Analysis of Fatty Acid Desaturases in Soybean (Glycine max)

Characterization of the LhcSR Gene Under Light and Temperature Stress in the Green Alga Ulva linza

Molecular cloning of two novel stearoyl-acyl desaturase genes from winterness wheat

Contact Info

Product

Resources

About