Cloning and Characterization of a Balsam Pear Class I Chitinase Gene (<i>Mcchit1</i>) and Its Ectopic Expression Enhances Fungal Resistance in Transgenic Plants

Xiao, Yazhong; Li, Xianbi; Yang, Xingyong; Luo, Ming; Hou, Lei; Guo, Shaohui; Luo, Xiaoying; Pei, Yan

doi:10.1271/bbb.60658

Cited by 51 publications

(41 citation statements)

References 46 publications

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“…Cell wall degrading enzymes including chitinases from biocontrol fungi belonging to the genus Trichoderma have been demonstrated to have high antifungal activity against a wide range of economically important aerial and soil-borne plant pathogenic fungi (Carsolio et al 1999;Gokul et al 2000;Adams 2004;Harighi et al 2006). The chitinase enzymes are fungicidal (Jayaraj & Punja 2007;Xiao et al 2007) and non-toxic to plants, animals, and higher vertebrates (Lorito et al 1996). Some research has been conducted to develop transgenic crop plants that have increased expression levels of chitinase genes in hopes of producing fungal disease resistant varieties (Liu et al 2004;Deng et al 2007;Gentile et al 2007;Jayaraj & Punja 2007;Xiao et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The chitinase enzymes are fungicidal (Jayaraj & Punja 2007;Xiao et al 2007) and non-toxic to plants, animals, and higher vertebrates (Lorito et al 1996). Some research has been conducted to develop transgenic crop plants that have increased expression levels of chitinase genes in hopes of producing fungal disease resistant varieties (Liu et al 2004;Deng et al 2007;Gentile et al 2007;Jayaraj & Punja 2007;Xiao et al 2007). It has been shown that chitinase enhances fungal disease resistance in different species (Gentile et al 2007), and rice against Rhizoctonia solani and Magnaporthe grisea (Liu et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Theoretically, the foreign genes can be transferred into the genomes of plants without altering any other agro-economically important traits (Chang et al 2002). Since chitin is a structural polymer in many fungal pathogens, the chitinases which are potential antifungal agents through their chitin degradation activity, are excellent candidates for controlling the fungal pathogens development (Kalai et al 2006;Maximova et al 2006;Gentile et al 2007;Jayaraj & Punja 2007;Xiao et al 2007;Pasonen et al 2008).…”

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confidence: 99%

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Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum

Solgi¹,

Moradyar²,

Zamani³

et al. 2015

Plant Prot. Sci.

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Solgi T., Moradyar M., Zamani M.R., Motallebi M. (2015): Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum. Plant Protect. Sci., 51: 6-12.An endochitinase gene (chit33-cDNA) from the biocontrol fungus Trichoderma atroviride was overexpressed under the CaMV35S constitutive promoter in canola (R line Hyola 308). Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens. The insertion of the transgene was verified by PCR and Southern blotting. The transgenic over-expression approach was used in order to investigate antifungal activity of expressed Chit33 on Sclerotinia sclerotiorum. Antifungal activity was detected in transgenic canola using detached leaf assay. Lesion sizes of transgenic canola caused by S. sclerotiorum were significantly retarded when compared to non-transgenic canola plant.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum

Solgi¹,

Moradyar²,

Zamani³

et al. 2015

Plant Prot. Sci.

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“…Transferred chitinase genes originate either from plants (Lin et al 1995, Kishimoto et al 2004, Chye et al 2005, Takashaki et al 2005, Tohidfar et al 2005, Vellicce et al 2006, Xiao et al 2007, He et al 2008, fungi (Terakawa et al 1997, Mora and Earle 2001, Kumar et al 2009, Kern et al 2010, Prasad et al 2012 or viruses (Corrado et al 2008). …”

Section: Plants Transformed With Chitinase Genesmentioning

confidence: 99%

Clonal variation in Scots pine (Pinus sylvestris) and in transgenic silver birch (Betula pendula)

Niskanen¹

2013

Diss. For.

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“…The most common cytosolic type I RIP from the endosperm of cereal grains do not act on plant ribosomes but can affect foreign ribosomes, such as those of fungi (Stirpe et al, 1992;Hartley et al, 1996). Several transgenic plants harbouring PR genes have already been produced, aiming at producing fungal disease-resistant varieties in a variety of crops (Jach et al, 1995;Tabei et al, 1997;Bieri et al, 2000;Datta et al, 2001;Jayaraj & Punja, 2007;Xiao et al, 2007;Almasia et al, 2008;Esfahani et al, 2010), including the transgenic tobacco that was engineered with the RIP encoding DNA sequence of barley and exhibits resistance to R. solani (Logeman et al, 1992). Many of the PR proteins may act synergistically in vivo and also enhanced inhibition of fungal growth when tested in combination in vitro (Jach et al, 1995;Chye et al, 2005).…”

Section: Bacterial Strain and Growth Conditionsmentioning

confidence: 99%

Enhanced resistance to Rhizoctonia solani by combined expression of chitinase and Ribosome Inactivating Protein in transgenic potatoes (Solanum tuberosum L.)

Mahmoud¹,

Chikh-Rouhou²,

Boughalleb³

et al. 2012

Span. j. agric. res.

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Potato (Solanum tuberosum L.) is susceptible to many fungal pathogens including Rhizoctonia solani. In the present study, the potato cultivar Desirée was transformed via Agrobacterium tumefaciens strain GV3101 containing the binary plasmid pGJ132 harboring both the chitinase (chiA) and rip30 genes. The potato leaf disc was used as an explant for transformation. PCR, Southern blot and Western blot were used for characterization of the transgenic plants. In this study it was shown that not all the plants developed in selective medium were positive for the corresponding gene using the PCR technique. Southern blot analysis confirmed that transgenic plants integrated 2-3 copies of chiA and rip30 genes respectively into their genome. The expression of the CHIA and RIP30 proteins was confirmed in the leaf extracts of the transgenic clones by Western blot analysis. Transgenic potato plants expressing rip30 and chiA genes showed enhanced resistance to R. solani in a greenhouse assay.Additional key words: fungal resistance; genes; proteins; southern blot; western blot. ResumenResistencia aumentada a Rhizoctonia solani por la expresión combinada de quitinasa y proteínas inactivantes de los ribosomas en patatas transgénicas La patata (Solanum tuberosum L.) es susceptible a muchos hongos fitopatógenos, incluyendo Rhizoctonia solani. En el presente estudio, se transformó el cultivar de patata 'Desirée' mediante Agrobacterium tumefaciens, cepa GV3101, que contiene el plásmido binario pGJ132 que alberga los genes quitinasa (chiA) y rip30. Se utilizaron discos de hojas como explante para la transformación de plantas. Se utilizaron las técnicas de PCR, Southern y Western blot para la caracterización de las plantas transgénicas. En este estudio se demostró, mediante PCR, que no todas las plantas que se desarrollaron en medio selectivo fueron positivas para el gen correspondiente. El análisis de Southern blot confirmó que las plantas transgénicas integraron en su genoma 2-3 copias de los genes chiA y rip30. Se llevó a cabo un ensayo de invernadero para evaluar la resistencia a R. solani de los clones transgénicos que expresan los transgenes. Las plantas transgénicas que expresan los genes rip30 y chiA mostraron una resistencia completa a R. solani.

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Cloning and Characterization of a Balsam Pear Class I Chitinase Gene (Mcchit1) and Its Ectopic Expression Enhances Fungal Resistance in Transgenic Plants

Cited by 51 publications

References 46 publications

Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum

Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum

Clonal variation in Scots pine (Pinus sylvestris) and in transgenic silver birch (Betula pendula)

Enhanced resistance to Rhizoctonia solani by combined expression of chitinase and Ribosome Inactivating Protein in transgenic potatoes (Solanum tuberosum L.)

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