Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum

Solgi, Tara; Moradyar, Mehdi; Zamani, Mohammadreza; Motallebi, Mostafa

doi:10.17221/83/2013-pps

Cited by 17 publications

(8 citation statements)

References 30 publications

(33 reference statements)

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“…The current scenario of plant pathogenesis focuses on the development of disease-resistant transgenic plants by the incorporation of chitinases encoding genes from any species to any plant in order to boost the disease resistance in plants (Table 1). The transformation of canola by an endochitinase gene, chit33 from Trichoderma atroviride, had led to increased resistance towards Sclerotinia sclerotiorum [68]. A detached leaf assay following chit33 expression illustrated decreased lesion sizes as compared to non-transgenic canola.…”

Section: Application Of Chitinases In Agriculturementioning

confidence: 99%

Chitinases—Potential Candidates for Enhanced Plant Resistance towards Fungal Pathogens

et al. 2018

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Crop cultivation is crucial for the existence of human beings, as it fulfills our nutritional requirements. Crops and other plants are always at a high risk of being attacked by phytopathogens, especially pathogenic fungi. Although plants have a well-developed defense system, it can be compromised during pathogen attack. Chitinases can enhance the plant's defense system as they act on chitin, a major component of the cell wall of pathogenic fungi, and render the fungi inactive without any negative impact on the plants. Along with strengthening plant defense mechanisms, chitinases also improve plant growth and yield. Chitinases in combination with recombinant technology can be a promising tool for improving plant resistance to fungal diseases. The applicability of chitinase-derived oligomeric products of chitin further augment chitinase prospecting to enhance plant defense and growth.

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Section: Application Of Chitinases In Agriculturementioning

confidence: 99%

Chitinases—Potential Candidates for Enhanced Plant Resistance towards Fungal Pathogens

et al. 2018

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“…The rationale for adding a sporamin-encoding sequence to the expression vector in this study was to simultaneously enhance fungal and insect resistance, sporamin having been characterized as a potent inhibitor of invertebrate pests in other species (Cai et al, 2003;Chen et al, 2006). In the second of these studies, a chitinase gene (Chit33) from the fungus Trichoderma viride (which is able to feed on other fungi by utilizing chitin) was expressed, leading to reductions in lesion size in transgenic lines of between 30% and 62.5% (Solgi et al, 2015).…”

Section: Direct Inhibitors Of Fungal Growthmentioning

confidence: 99%

The control of sclerotinia stem rot on oilseed rape (Brassica napus): current practices and future opportunities

2016

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Sclerotinia stem rot (SSR) caused by the phytopathogenic fungus Sclerotinia sclerotiorum is a major disease of oilseed rape (Brassica napus). During infection, large, white/grey lesions form on the stems of the host plant, perturbing seed development and decreasing yield. Due to its ability to produce long‐term storage structures called sclerotia, S. sclerotiorum inoculum can persist for long periods in the soil. Current SSR control relies heavily on cultural practices and fungicide treatments. Cultural control practices aim to reduce the number of sclerotia in the soil or create conditions that are unfavourable for disease development. These methods of control are under increased pressure in some regions, as rotations tighten and inoculum levels increase. Despite their ability to efficiently kill S. sclerotiorum, preventative fungicides remain an expensive gamble for SSR control, as their effectiveness is highly dependent on the ability to predict the establishment of microscopic infections in the crop. Failure to correctly time fungicide applications can result in a substantial cost to the grower. This review describes the scientific literature pertaining to current SSR control practices. Furthermore, it details recent advances in alternative SSR control methods including the generation of resistant varieties through genetic modification and traditional breeding, and biocontrol. The review concludes with a future directive for SSR control on oilseed rape.

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“…Some important factors such as pH, aeration, and temperature have great influence on the production of chitinase described by Bhushan 1998 [41][42][43]. To increase the chitinases Sclerotinia minor [44][45][46].…”

Section: Methods Of Production Of Chitinasementioning

confidence: 99%

Chitinases Potential as Bio-Control

Iqbal

2019

BJSTR

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The richest second polysaccharide in nature later than cellulose is chitin. It is present in the exoskeleton of yeast, fungi, algae and insects. It is also present in the inner structure of different vertebrates. The degradation of chitin is done by chitinases enzymes. In the environment nitrogen and carbon are generated by the contribution of chitinases. In biotechnological applications chitinolytic enzymes and chitin have gaining their significance. In the field of agriculture for the control of pathogens particularly the chitinases demoralized. Some human diseases particularly such as asthma, chitinases have a broad use for human health care. For the preparation of pharmaceutically significant chito-oligosaccharides and preparation of single cell protein, N-acetyl D glucosamine, separation of protoplast from yeast & fungi, for the handling of chitinous squander, to stop the pathogenic fungi and to stop the morphogenesis and mosquitoes etc chitinases has wider range applications. We are discussed a different type of chitinases and the chitinases present in a number of organisms for example plants, bacteria, mammals and fungi in this review.

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Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum

Cited by 17 publications

References 30 publications

Chitinases—Potential Candidates for Enhanced Plant Resistance towards Fungal Pathogens

Chitinases—Potential Candidates for Enhanced Plant Resistance towards Fungal Pathogens

The control of sclerotinia stem rot on oilseed rape (Brassica napus): current practices and future opportunities

Chitinases Potential as Bio-Control

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