Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase.

Estruch, Juan José; Kadwell, Sue H.; Merlin, Ellis; Crossland, Lyle

doi:10.1073/pnas.91.19.8837

Cited by 180 publications

(101 citation statements)

References 18 publications

(17 reference statements)

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“…Alignment of the CCK1 junction domain with that of other CDPKs and the relative homologies of the kinase-and calmodulin-like domains. A Three or more identical amino acids in the junction domains of CCK1, CDPKa (Harper et al 1991), AK1 (Harper et al 1993), AtCDPK1 (Urao et al 1994), SPK1 (Kawasaki et al 1993), ZmCDPK (Estruch et al 1994) and DcCDPK (Suen and Choi 1991) are shown. B Phylogenetic relations of the kinase domains of the CDPKs from Chlamydomonas (CCK1), soybean (CDPKa), Arabidopsis (AK1) and carrot (DcCDPK) are shown compared with human PKA, PKCa (Finkenzeller et al 1990), rat MLCK (Roush et al 1988) and mouse calcium/calmodulin-dependent kinase II (Karls et al 1992).…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, actin has been shown to be concentrated in these structures (Kalshoven et al 1994); therefore, an interaction between CDPKs and the cytoskeleton is possible. The presence of CDPKs in pollen tubes of maize (Estruch et al 1994), where the actin cytoskeleton is implicated in outgrowth of these structures could indicate a common association of CDPKs with the cytoskeleton. In other plant systems, the interrelation between the cytoskeleton and CDPKs has also been studied (Putnam-Evans et al 1989;McCurdy and Harmon 1992).…”

Section: Discussionmentioning

confidence: 99%

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Characterisation and cloning of a calmodulin-like domain protein kinase from Chlamydomonas moewusii (Gerloff )

Siderius

Henskens

Porto-Leblanche

et al. 1997

Planta

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General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Download date: 12 May 2018Abstract. Calcium-stimulated protein kinase activity in the¯agella of the green alga Chlamydomonas moewusii (Gerlo ) was characterised. Using SDS-PAGE and an on-blot phosphorylation assay, a 65-kDa protein was identi®ed as the major calcium-stimulated protein kinase. Its activity was directly stimulated by calcium, a characteristic of the calmodulin-like domain protein kinases (CDPKs). Monoclonal antibodies raised against the CDPKa from soybean cross-reacted with the 65-kDa protein in the¯agella, and also with other proteins in thē agellum and cell body. The same monoclonal antibodies were used to screen a C. moewusii cDNA expression library in order to isolate CDPK cDNAs from C. moewusii. The CCK1 cDNA encodes a protein with a kinase and calmodulin-like domain linked by a junction domain typical of CDPKs. From Southern analyses, evidence was obtained for a CDPK gene family in C. moewusii and C. reinhardtii.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterisation and cloning of a calmodulin-like domain protein kinase from Chlamydomonas moewusii (Gerloff )

Siderius

Henskens

Porto-Leblanche

et al. 1997

Planta

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“…One of the maize CDPK genes (designated by us as ZmCPK7 according to the nomenclature by Ma et al 2013), also similar to AtCPK17/AtCPK34, is expressed specifically during late stages of pollen development. Specific inhibition of the ZmCPK7 expression by antisense oligonucleotide resulted in impaired germination and growth of pollen tube (Estruch et al 1994). According to Ray et al (2007), OsCPK2 and 14 from rice that show homology to PiCDPK1 and AtCPK17 and 34, and OsCPK25 and 26, highly similar to ZmCPK7, are expressed during late panicle development and thus could be involved in a similar function during pollen maturation and/or pollen tube growth.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have demonstrated that CDPKs play key roles in the regulation of plant growth, development, and abiotic and biotic stress resistance (for review see Klimecka and Muszyńska 2007). Some CDPK genes have been shown to be involved in pollen tube growth (Estruch et al 1994), root development (Ivashuta et al 2005) and cell division and differentiation (Yoon et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis and expression profiling of calcium-dependent protein kinases in potato (Solanum tuberosum)

et al. 2017

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“…Genes which are transiently and specifically transcribed during pollen development are assumed to play a crucial role in the developmental processes. Summarizing, genes involved in pollen development can be classified, according to expression pattern as early [dO, 28,35], and late genes [4,7,21,32,39,42]. Additionally, genes transcribed in both stages have been isolated [ 1,24,33].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a microspore-specific gene from tobacco

et al. 1996

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The characterization of a gene with a unique microspore-specific expression pattern is reported. Isolated microspores from tobacco were used to synthesize a cDNA library. Clones that did not hybridize to leaf cDNA were further characterized by northern analysis. One clone proved to be a microspore-specific cDNA, representing a transcript of 650 nt. The corresponding gene, NTM19 (Nicotiana tabacum microspore-specific), was isolated and its sequence analysed. The gene encodes a protein of 10.8 kDa with a pI of 6.92 and a putative signal sequence at the N-terminus. A localization study revealed a unique spatial and temporal distribution. The transcript was only detected in the unicellular microspore. No hybridization signals were observed in other pollen developmental stages, nor in the surrounding anther tissues or other vegetative tissues of the plant. Therefore it can be concluded that NTM 19 is a gene with a highly microspore-specific character according to both localization and stage of expression. Southern blot analysis demonstrated the presence of a small gene family. The occurrence of TNM 19 was investigated in a range of closely and distantly related species and was found to be present in other solanaceous species, including the ancestors of tobacco and in a monocot species.

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Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase.

Abstract: A calcium-dependent calmodulin-independent protein kinase (CDPK) has been cloned from maize (Zea

Cited by 180 publications

References 18 publications

Characterisation and cloning of a calmodulin-like domain protein kinase from Chlamydomonas moewusii (Gerloff )

Characterisation and cloning of a calmodulin-like domain protein kinase from Chlamydomonas moewusii (Gerloff )

Genome-wide analysis and expression profiling of calcium-dependent protein kinases in potato (Solanum tuberosum)

Isolation and characterization of a microspore-specific gene from tobacco

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