Genome-wide analysis and expression profiling of calcium-dependent protein kinases in potato (Solanum tuberosum)

Gromadka, Robert; Cieśla, Jarosław M.; Olszak, K.; Szczegielniak, Jadwiga; Muszyńska, Grażyna; Polkowska‐Kowalczyk, Lidia

doi:10.1007/s10725-017-0341-9

Cited by 22 publications

(7 citation statements)

References 65 publications

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“…All CrCDPK genes harbor a typical structure containing protein kinase and Ca 2+ binding domains in C. reinhardtii ( Figure 1 ), and all of them contain four EF-hand motifs except for CrCDPK10 , which only has two EF-hands. In other plant species, such as tomato and potato, similar results were obtained: some CDPK genes have less than four EF-hands (Gromadka et al, 2018; Hu et al, 2016a; Hu et al, 2016b). Boudsocq et al (2012) reported that the AtCPK25 gene is Ca 2+ independent owing to the absence of functional EF-hands in Arabidopsis .…”

Section: Discussionsupporting

confidence: 65%

“…For instance, 34 and 31 CDPKs have been identified in Arabidopsis and rice genomes, respectively (Hrabak et al, 2003; Ray et al, 2007). In addition, there are 20 CDPKs in wheat genome (Li et al, 2008), 23 in potato (Gromadka et al, 2018), 27 in cassava (Hu et al, 2016a), 29 in tomato (Hu et al, 2016b), 30 in poplar (Zuo et al, 2013), 40 in maize (Kong et al, 2013), and 41 in cotton (Liu et al, 2014). The biological functions of CDPKs, including pollen tube development (AtCPK2/AtCPK11/AtCPK17/AtCPK20/AtCPK24/AtCPK34) (Gutermuth et al, 2013; Zhao et al, 2013), response to environmental stresses (AtCPK1/AtCPK3/AtCPK4/AtCPK5/AtCPK6/AtCPK8/AtCPK10/AtCPK11/AtCPK13/AtCPK21) (Choi et al, 2005; Mori et al, 2006; Zhu et al, 2007; Boudsocq et al, 2010; Franz et al, 2011; Zhou et al, 2015), and plant growth regulation (AtCPK12/AtCPK28) (Zhao et al, 2011; Matschi et al, 2013), have been well characterized in Arabidopsis .…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

Fei

Dai

et al. 2019

Front. Plant Sci.

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Calcium-dependent protein kinases (CDPKs) are recognized as important calcium (Ca2+) sensors in signal transduction and play multiple roles in plant growth and developmental processes, as well as in response to various environmental stresses. However, little information is available about the CDPK family in the green microalga Chlamydomonas reinhardtii. In this study, 15 CrCDPK genes were identified in C. reinhardtii genome, and their functions in nitrogen (N) deficiency-induced oil accumulation were analyzed. Our results showed that all CrCDPK proteins harbored the typical elongation factor (EF)-hand Ca2+-binding and protein kinase domains. Phylogenetic analysis revealed that these CrCDPKs were clustered into one group together with a subclade of several CPKs from Arabidopsis and rice, clearly separating from the remaining AtCPKs and OsCPKs. These genes were located in 10 chromosomes and one scaffold of C. reinhardtii and contained 6–17 exons. RNA sequencing and quantitative reverse transcription (qRT)-PCR assays indicated that most of these CrCDPKs were significantly induced by N deficiency and salt stress. Lanthanum chloride (LaCl3), a plasma membrane Ca2+ channel blocker, limited oil accumulation in C. reinhardtii under N-deficient conditions, suggesting that Ca2+ was involved in N deficiency-induced oil accumulation. Furthermore, RNA interference (RNAi) silencing analyses demonstrated that six CrCDPKs played positive roles and three CrCDPKs played negative roles in N deficiency-induced oil accumulation in C. reinhardtii.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

Fei

Dai

et al. 2019

Front. Plant Sci.

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“…The assay of gene expression revealed that interference-expression of StCDPK14 resulted in a decline levels of its own transcript and StRbohA - H throughout the wound healing period in tubers ( Figures 6 , 7A ), which might account for the involvement of StCDPK14 in the activation process of RBOH during wound healing in potato tubers. Previous studies also documented the relationship between CDPKs and Rboh proteins, that is, the phosphorylation of StRboh by StCDPK ( Gromadka et al, 2018 ) and the oxidative burst resulting from increased CDPK expression in potato ( Polkowska-Kowalczyk et al, 2004 ). Therefore, an assumption was that StCDPK14 activated the Rboh activity via a specific event to regulate ROS generation during wound healing of potato tubers.…”

Section: Discussionmentioning

confidence: 99%

The Interaction Between StCDPK14 and StRbohB Contributes to Benzo-(1, 2, 3)-Thiadiazole-7-Carbothioic Acid S-Methyl Ester-Induced Wound Healing of Potato Tubers by Regulating Reactive Oxygen Species Generation

Jiang

et al. 2021

Front. Plant Sci.

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Reactive oxygen species (ROS) production is essential for both physiological processes and environmental stress in diverse plants. Previous studies have found that benzo-(1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH)-inducible ROS were associated with wound healing of potato tubers. Calcium-dependent protein kinases (CDPKs), the important calcium receptors, are known to play a crucial part in plant development and adaptation to abiotic stresses. However, whether CDPK-mediated ROS generation induced by BTH is involved in wound healing is elusive. In this study, we measured Solanum tuberosum CDPKs (StCDPKs) expression using real-time PCR, and it was found that the transcriptional levels of StCDPKs from BTH-treated tissues were significantly induced, among which StCDPK14 presented the most increased level. Subcellular localization results showed that StCDPK14 is located in the nucleus and membrane. The transgenic potato plants and tubers were developed using interference-expression of StCDPK14 by Agrobacterium tumefaciens–mediated transformation. The St respiratory burst oxidase homologs (StRbohs) expression showed a remarkable decrease in StCDPK14 transgenic tubers, notably, H2O2 content and suberin deposition were also significantly declined. To confirm the relationship between StCDPK14 and StRbohB, yeast-two-hybrid and bimolecular fluorescence complementation were used to examine the interaction, and it was shown that StCDPK14 interacted with the specific Ca2 + -binding motif (helix-loop-helix, called EF-hand) of StRbohB N-terminus. The above results unraveled that StCDPK14 functions in ROS generation via interacting with StRbohB during wound healing of potato tubers.

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“…Many DEGs could be annotated to kinase activity and protein kinase activity. Calcium-dependent protein kinases (CDPKs or CPKs) have been found to be involved in growth and developmental processes as well as in defense against diverse environmental stresses [59]. Reactive oxygen species (ROS) have been implicated in plant innate immunity and calcium-dependent protein kinases with regulation of the production of ROS by potato NADPH oxidase [60].…”

Section: Protein Kinases May Participate In the Interaction Between Qingshu 9 And P Infestansmentioning

confidence: 99%

Serial Transcriptome Analysis Reveals Genes Associated with Late Blight Resistance in Potato Cultivar Qingshu 9

Zhou

et al. 2021

Agronomy

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The late blight caused by Phytophthora infestans (P. infestans) has been a major constraint for worldwide sustainable potato production. Chinese potato cultivar Qingshu 9 has shown excellent field performance against late blight. To understand the mechanism underlying its resistance, the transcriptomic dynamics of Qingshu 9 infected with P. infestans was systematically investigated to identify the resistance-related genes. In total, 2027 shared differentially expressed genes (DEGs) were identified when infected with P. infestans at 24, 48 and 72 h post-inoculation. Based on k-mean cluster analysis, the DEGs showed four types of expression patterns, suggesting their divergent functions in response to P. infestans infection. KEGG enrichment analysis showed that the significantly enriched DEGs were involved in the biosynthesis of secondary metabolites, plant–pathogen interaction, and photosynthesis. Furthermore, 980 transcription factor genes belonging to 68 families were found in the DEGs, of which AP2-EREBP and MYB genes were the most abundant. Moreover, many genes related to disease resistance showed differential expression during infection. Finally, the expression of nine DEGs was validated by quantitative real-time PCR. These results provide valuable information for understanding late resistance in potato cultivar Qingshu 9.

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Genome-wide analysis and expression profiling of calcium-dependent protein kinases in potato (Solanum tuberosum)

Cited by 22 publications

References 65 publications

Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

The Interaction Between StCDPK14 and StRbohB Contributes to Benzo-(1, 2, 3)-Thiadiazole-7-Carbothioic Acid S-Methyl Ester-Induced Wound Healing of Potato Tubers by Regulating Reactive Oxygen Species Generation

Serial Transcriptome Analysis Reveals Genes Associated with Late Blight Resistance in Potato Cultivar Qingshu 9

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