Cloning and characterisation of a basic IAA oxidase associated with root induction in Vitis vinifera

Vatulescu, Ada D; Fortunato, Ana S.; Sá, M. Cláudia; Amâncio, Sara; Ricardo, Cândido Pinto; Jackson, P

doi:10.1016/j.plaphy.2004.06.009

Cited by 27 publications

(17 citation statements)

References 35 publications

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“…Ferrer et al (1990) have proposed a model where PODs involved in lignification may utilize H 2 O 2 generated by other PODs through the oxidative cycle at expense of IAA. It has also been reported that basic IAA-POD activity was mainly associated with early phases of root growth restriction (Vatulescu et al 2004). Though, under severe stress the level of ROS may increase to detrimental concentration for cells.…”

Section: Discussionmentioning

confidence: 91%

Elevated indole-3-acetic acid peroxidase activity is involved in the cadmium-induced hydrogen peroxide production in barley root tip

et al. 2010

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Section: Discussionmentioning

confidence: 91%

Elevated indole-3-acetic acid peroxidase activity is involved in the cadmium-induced hydrogen peroxide production in barley root tip

et al. 2010

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“…Therefore, oxidative decarboxylation of auxin does play a major role in rooting of cuttings. The significance of decarboxylation during rooting is also shown by an increase of apoplastic peroxidase with high IAA-oxidase activity during rooting of Vitis microshoots (Vatulescu et al 2004). In apple slices, peroxidase is released into the medium from the cut surface and there is no or only little release from the intact epidermis (Huisman and De Klerk, unp.…”

Section: Discussionmentioning

confidence: 99%

Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied indoleacetic acid in Malus ‘Jork 9’

Klerk¹,

Guan²,

Huisman³

et al. 2011

Plant Growth Regul

135

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Stem slices (1-mm thick) cut from apple microshoots were cultured on a modified Murashige-Skoog medium with indole-3-acetic acid (IAA) or a-naphthaleneacetic acid (NAA), and increasing concentrations of various phenolic compounds. Both auxins were added at a concentration suboptimal for rooting. Indole-3-acetic acid is metabolized through oxidation and conjugation but NAA through conjugation only; which might have affected the results. With IAA, all tested orthodiphenols, paradiphenols and triphenols promoted adventitious root formation from the stem slices. Ferulic acid (FA, a methylated orthodiphenol) had the largest effect and increased the number of adventitious roots from 0.9 to 5.8. With NAA there was little or no promotion after addition of phenolics. Phloroglucinol (a triphenol) and FA were examined in detail. Their effects on the dose-response curve of IAA and the timing of their action indicated that both acted as antioxidants protecting IAA from decarboxylation and the tissue from oxidative stress. Experiments with carboxyl-labelled IAA showed that IAA was massively decarboxylated by the slices and that decarboxylation was strongly reduced by phenolics. Decarboxylation was to a great extent attributable to the wound response and did not occur to such an extent in non-wounded plant tissues. In shoots, FA promoted little rooting. Slices were cultured on top of the medium and shoots were stuck into the medium. Possibly, the anaerobic conditions in the medium near the basal part of the stem of shoots reduced the wound response and consequently decarboxylation of IAA. The monophenolic compound salicylic acid (SA) promoted IAA decarboxylation. Accordingly, SA reduced rooting when added during the initial days of the rooting process (the period during which auxin enhances rooting), and promoted outgrowth of root primordia later on (the period during which auxin inhibits rooting).

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“…In Vitis vinifera a peroxidase (VvPrx07) with IAA oxidase activity was identified (Vatulescu et al, 2004). This peroxidase seemed to control IAA levels during root initiation and development.…”

Section: Auxinmentioning

confidence: 99%

Specific functions of individual class III peroxidase genes

Cosio

Dunand

2009

Journal of Experimental Botany

364

288

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In higher plants, class III peroxidases exist as large multigene families (e.g. 73 genes in Arabidopsis thaliana). The diversity of processes catalysed by peroxidases as well as the large number of their genes suggests the possibility of a functional specialization of each isoform. In addition, the fact that peroxidase promoter sequences are very divergent and that protein sequences contain both highly conserved domains and variable regions supports this hypothesis. However, two difficulties are associated with the study of the function of specific peroxidase genes: (i) the modification of the expression of a single peroxidase gene often results in no visible mutant phenotype, because it is compensated by redundant genes; and (ii) peroxidases show low substrate specificity in vitro resulting in an unreliable indication of peroxidase specific activity unless complementary data are available. The generalization of molecular biology approaches such as whole transcriptome analysis and recombinant DNA combined with biochemical approaches provide unprecedented tools for overcoming these difficulties. This review highlights progress made with these new techniques for identifying the specific function of individual class III peroxidase genes taking as an example the model plant A. thaliana, as well as discussing some other plants.

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Cloning and characterisation of a basic IAA oxidase associated with root induction in Vitis vinifera

Cited by 27 publications

References 35 publications

Elevated indole-3-acetic acid peroxidase activity is involved in the cadmium-induced hydrogen peroxide production in barley root tip

Elevated indole-3-acetic acid peroxidase activity is involved in the cadmium-induced hydrogen peroxide production in barley root tip

Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied indoleacetic acid in Malus ‘Jork 9’

Specific functions of individual class III peroxidase genes

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