Elevated indole-3-acetic acid peroxidase activity is involved in the cadmium-induced hydrogen peroxide production in barley root tip

Halušková, Ľubica; Valentovičová, Katarína; Huttová, Jana; Mistrı́k, Igor; Tamás, Ladislav

doi:10.1007/s10725-010-9486-5

Cited by 5 publications

(2 citation statements)

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“…It has previously been demonstrated that IAA induced root growth inhibition by the generation of H 2 O 2 (Joo et al 2001). Our results showed that exogenously applied IAA induced a nonsignificant increase of soluble AAO but probably due to the IAA-induced H 2 O 2 production in the differentiation zone through the action of IAA degrading peroxidases (Halušková et al 2010) caused a marked inhibition of ionically-bound isoforms.…”

Section: Discussionsupporting

confidence: 59%

Abiotic stress–induced inhibition of root growth and ascorbic acid oxidase activity in barley root tip is associated with enhanced generation of hydrogen peroxide

et al. 2011

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Using short-term treatments, the aim of this study was to analyze the role of hydrogen peroxide in the regulation of AAO activity during Cd, Cu or IAA treatments in barley root tips. For analysis individual barley root segments were obtained by the gradual cutting of each root from the tip to the base 1, 2, 3 or 6 h after short-term treatments. Already a short 30 min exposure of barley roots to Cd induced significant root growth inhibition in a Cd concentration dependent manner, which was accompanied by a marked reduction of AAO activity. At Cu concentration which had no effect on the root growth a significant increase in AAO activity was observed. This increased AAO activity was detected only in ionically-bound CW fraction. In contrast, Cu at higher concentration and IAA inhibited both ionically-bound CWAAO isozymes. Prompt inhibition of AAO activity immediately after short-term treatment was observed only in the case of H 2 O 2 treatment suggesting that H 2 O 2 may act as an inhibitor of AAO. This was further supported by the observation that all Cd-, Cu-or IAA-induced root growth and AAO activity inhibition in barley roots was connected with an elevated production of H 2 O 2 .

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Section: Discussionsupporting

confidence: 59%

Abiotic stress–induced inhibition of root growth and ascorbic acid oxidase activity in barley root tip is associated with enhanced generation of hydrogen peroxide

et al. 2011

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“…An increase in the abundance of proteins associated with stress defense has been identified in some higher plants, e.g. rice, Arabidopsis and barley, subjected to heavy-metal stresses, and upregulation of these proteins may play an important role in minimizing the ROS damage triggered by heavy metals such as Hg. ,, Peroxidase, glutathione synthesized-relate enzymes, cytochrome P450 family members, glutaredoxin, and thioredoxin have been reported to play key roles in ROS scavenging systems under abiotic stress conditions. − In this study, 15 peroxidases (8 in roots, 7 in leaves), 3 glutathione synthesized-related enzymes in leaves, 3 cytochrome P450 family members in leaves, and 1 thioredoxin in leaves were upregulated markedly by Hg stress (Tables and ). This suggests that the contents of various antioxidants, such as antioxidant enzymes, were upregulated in the roots and leaves of wheat seedlings to combat the oxidative stress induced by Hg.…”

Section: Resultsmentioning

confidence: 79%

Hg-Responsive Proteins Identified in Wheat Seedlings Using iTRAQ Analysis and the Role of ABA in Hg Stress

et al. 2014

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Wheat seedlings exposed to 100 μM HgCl2 for 3 days exhibited high-level mercury (Hg) accumulation, which led to inhibited growth, increased lipid peroxidation, and disrupted cellular ultrastructures. And root growth and ultrastructural changes of wheat seedlings were inhibited more severely than those of leaves. To identify the wheat protein response to Hg stress, the iTRAQ method was used to determine the proteome profiles of the roots and leaves of wheat seedlings exposed to high-Hg conditions. 249 proteins were identified with significantly altered abundance. 117 were found in roots and 132 in leaves. These proteins were classified into signal transduction, stress defense, carbohydrate metabolism, protein metabolism, energy production, and transport functional groups. The majority of proteins identified in Hg-stressed roots and leaves displayed differently altered abundance, revealing organ-specific differences in adaption to Hg stress. Pathway Studio software was used to identify the Hg-responsive protein interaction network that included 49 putative key proteins, and they were potentially regulated by abscisic acid (ABA). Exogenous ABA application conferred protection against Hg stress and increased activities of peroxidase enzyme, suggesting that it may be an important factor in the Hg signaling pathway. These findings can provide useful insights into the molecular mechanisms of Hg responses in higher plants.

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Interference of Heavy Metal Toxicity with Auxin Physiology

Elobeid

Polle²

2011

Metal Toxicity in Plants: Perception, Signaling and Remediation

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Elevated indole-3-acetic acid peroxidase activity is involved in the cadmium-induced hydrogen peroxide production in barley root tip

Cited by 5 publications

References 28 publications

Abiotic stress–induced inhibition of root growth and ascorbic acid oxidase activity in barley root tip is associated with enhanced generation of hydrogen peroxide

Abiotic stress–induced inhibition of root growth and ascorbic acid oxidase activity in barley root tip is associated with enhanced generation of hydrogen peroxide

Hg-Responsive Proteins Identified in Wheat Seedlings Using iTRAQ Analysis and the Role of ABA in Hg Stress

Interference of Heavy Metal Toxicity with Auxin Physiology

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