Cloned endothelium derived from autoimmune vascular disease retain structural and functional characteristics of normal endothelial cells

Moyer, Carolyn F.; Huggins, Edd; Sarantopoulos, Stefanie; Lewis, Jon C.; Sajuthi, Dondin; Biron, Christine A.; Reinisch, Carol L.

doi:10.1016/0014-4827(92)90462-h

Cited by 10 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immortal mouse cell lines were derived from endotheliomas [82] and the myocard [83]. Primary mouse EC were isolated from brain, ovary and lung [84] or pathologic mouse endothelium [85]. An immortalized rat bone marrow EC line [86], primary rat heart EC [87] and immortalized rat brain EC [88] were described.…”

Section: Discussionmentioning

confidence: 99%

Untitled

et al. 2001

View full text Add to dashboard Cite

Endothelial cells (EC) are currently used as in vitro model systems for various physiological and pathological processes, especially in angiogenesis research. Primary EC have a limited lifespan and display characteristics that differ from batch to batch due to their multidonor origin. In recent years many groups have established EC lines. This Review gives an overview of the advantages and disadvantages of currently available vascular EC lines. Its aim is to help the investigator to decide which cell line matches his or her research goal best. Truly immortalized cell lines are generally better characterized and more stable in their endothelial traits than EC that were given an extended life span. Presently the best characterized macro- and micro-vascular EC lines are EA.hy926 and HMEC-1, respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

Untitled

et al. 2001

View full text Add to dashboard Cite

show abstract

“…This can be explained by the relative difficulty in isolating and culturing vascular endothelium from these animals (10). In addition, the validity of a mouse model has been challenged due to interspecies differences in the phenotypes of vascular endothelium both at a resting state and after treatment with proinflammatory cytokines.…”

mentioning

confidence: 99%

Mouse Vascular Endothelium Activates CD8+ T Lymphocytes in a B7-Dependent Fashion

Kreisel¹,

Krupnick²,

Balsara³

et al. 2002

The Journal of Immunology

View full text Add to dashboard Cite

Despite several studies examining the contribution of allorecognition pathways to acute and chronic rejection of vascularized murine allografts, little data describing activation of alloreactive T cells by mouse vascular endothelium exist. We have used primary cultures of resting or IFN-γ-activated C57BL/6 (H-2b) vascular endothelial cells as stimulators and CD8+ T lymphocytes isolated from CBA/J (H-2k) mice as responders. Resting endothelium expressed low levels of MHC class I, which was markedly up-regulated after activation with IFN-γ. It also expressed moderate levels of CD80 at a resting state and after activation. Both resting and activated endothelium were able to induce proliferation of unprimed CD8+ T lymphocytes, with proliferation noted at earlier time points after coculture with activated endothelium. Activated endothelium was also able to induce proliferation of CD44low naive CD8+ T lymphocytes. Activated CD8+ T lymphocytes had the ability to produce IFN-γ and IL-2, acquired an effector phenotype, and showed up-regulation of the antiapoptotic protein Bcl-xL. Treatment with CTLA4-Ig led to marked reduction of T cell proliferation and a decrease in expression of Bcl-xL. Moreover, we demonstrate that nonhemopoietic cells such as vascular endothelium induce proliferation of CD8+ T lymphocytes in a B7-dependent fashion in vivo. These results suggest that vascular endothelium can act as an APC for CD8+ direct allorecognition and may, therefore, play an important role in regulating immune processes of allograft rejection.

show abstract

“…Aging may cause loss or alteration of organo r vascular bed-specific endothelial cell surface characteristics (Pauli et al, 1990;Belloni and Nicolson, 1988;Borsum et al, 1982), increased substrate adhesion and spreading as evidenced by the appearance of giant endothelial cells (Tokunaga et al, 1989), and lack of response to exogenous growth stimuli (Matsuda et al, 1992). This result is not surprising if one considers that microvascular endothelial cells usually isolated in small numbers have to pass through numerous population doublings before a continuous cell culture can be established or they need to be cloned by limiting dilution to eliminate undesirable contaminants requiring at least 20 population doublings before a culture has grown up to lo6 endothelial cells (Moyer et al, 1992;Thornton et al, 1983;Rosen et al, 1981).…”

mentioning

confidence: 99%

Senescence of aortic endothelial cells in culture: Effects of basic fibroblast growth factor expression on cell phenotype, migration, and proliferation

Augustin

Voss

Pauli

1993

Journal Cellular Physiology

View full text Add to dashboard Cite

Bovine aortic endothelial cells (BAEC) can be isolated in large numbers without major contamination by other cells and maintained in culture with a limited life span for about 100 population doublings. In order to study phenotypic changes of BAEC during long-term culture, stocks of different passages of BAEC were established and their morphological, migratory, and proliferative properties analyzed. Early-passage BAEC (passages 5-15) rapidly produce dense, cobblestone-like monolayers. Their growth beyond the monolayer configuration is characterized by the formation of an irregular network of spindle-shaped, crisscrossing BAEC growing either on top or beneath the monolayer, and by the assembly of elongated BAEC into well-differentiated capillary-like tubes. In contrast, senescent BAEC (passages 35-45) form perfect cobblestone monolayers that contain several, often multinucleated giant cells and a few capillary-like tubes but not the crisscrossing networks of their early-passage counterparts. The rates of BAEC migration and proliferation gradually decline during in vitro senescence. This decline is neutralized by exogenous basic fibroblast growth factor (bFGF) which elevates the migratory and proliferative capacities of early-passage and senescent BAEC to uniformly high levels. Northern blot analysis shows a gradual decline in bFGF message and an increase in laminin message during in vitro BAEC senescence. The present study supports the concept of autocrine growth regulation of BAEC and associates a decreased bFGF message with decreased rates of migration and proliferation as well as loss of the crisscrossing BAEC morphotype in senescent cultures.

show abstract

Cloned endothelium derived from autoimmune vascular disease retain structural and functional characteristics of normal endothelial cells

Cited by 10 publications

References 31 publications

Untitled

Untitled

Mouse Vascular Endothelium Activates CD8+ T Lymphocytes in a B7-Dependent Fashion

Senescence of aortic endothelial cells in culture: Effects of basic fibroblast growth factor expression on cell phenotype, migration, and proliferation

Contact Info

Product

Resources

About