Cloned Cauliflower Mosaic Virus DNA Infects Turnips (
            <i>Brassica rapa</i>
            )

SUMMARYNative DNA of both mirabilis mosaic virus (MMV) and the previously undescribed thistle mottle virus (ThMV) formed multiple bands when analysed by gel electrophoresis, thereby resembling DNA from other caulimoviruses. Denaturation showed that ThMV DNA had three discontinuities (one in one strand and two in the other) and that MMV DNA had four discontinuities which mapped in the same relative positions as those in DNA of figwort mosaic virus (FMV). DNA from carnation etched ring virus (CERV), FMV, MMV and ThMV was cloned in bacterial plasmids. Cloned full-length DNA of CERV, FMV or MMV was infective for plants.Comparisons by restriction mapping and Southern transfer hybridization among DNA from cauliflower mosaic virus, CERV, FMV, MMV and ThMV suggest that these viruses are distinct members of the caulimovirus group.

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“…5 e, j). Similar results were obtained following the passage of cloned CaMV DNA in plants (Howell et al, 1980).…”

Section: Molecular Cloningsupporting

confidence: 81%

Physical Mapping and Molecular Cloning of Caulimovirus DNA

Donson

Hull

1983

Journal of General Virology

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“…Cloned DNA of caulimoviruses is infectious when mechanically inoculated on host plants, after enzymic excision from the cloning vector (Howell et al, 1980;Lebeurier et al,, 1980), Because of the reported similarities between RTBV and caulimovirus DNAs (Jones et al, 1991), mechanical inoculation of cloned RTBV DNA, after suitable restriction enzyme digestions, was tested to see whether it was infectious. Another method of testing infectivity, bombarding rice seedlings with microprojectiles (Klein et al, 1987) coated with RTBV DNA (biolistics) was also tried.…”

Section: Introductionmentioning

confidence: 99%

Rice tungro bacilliform virus DNA independently infects rice after Agrobacterium-mediated transfer

Dasgupta

Hull

Eastop

et al. 1991

Journal of General Virology

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In nature, rice tungro disease is caused by an RNA and a DNA virus complex, but we have obtained an independently infectious clone of rice tungro bacilliform virus (RTBV) DNA. Infectivity could be demonstrated only when a more than unit-length copy was cloned in the Agrobacterium binary vector Binl9 and agroinoculated into rice plants. Rice plants thus agroinfected with cloned RTBV DNA showed typical symptoms of tungro disease, presence of viral DNA and bacilliform particles, and could be used as a source of virus to infect healthy plants by the green leafhopper (Nephotettix virescens). The importance of this infectious clone in understanding the molecular biology of RTBV and the rice tungro disease is discussed.

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“…The single break (A1) in the so-catled a-strand, the strand which is transcribed, is taken as the zero point of the conventional restriction map [5]; the two breaks in the complementary fl-strand, A3 and A2, are situated at 0.20 and 0.53 map units, respectively. CaMV DNA from which the discontinuities have been eliminated by cloning in pBR322 is fully infectious but the DNA isolated from the progeny of such infection is once again interrupted [6,7], suggesting that the breaks are essential for completion of the infectivity cycle and that a precise mechanism exists for introducing them into the viral DNA.…”

Section: Introductionmentioning

confidence: 99%