Clonal T-cell receptor ?-chain gene rearrangement by PCR-based GeneScan analysis in advanced cutaneous T-cell lymphoma: a critical evaluation

Paucity of FOXP3+ cells in skin and peripheral blood distinguishes Sézary syndrome from other cutaneous T-cell lymphomas

“…(Table 1). 19,21,22 In patients with MF, the cutaneous lymphomononuclear cell infiltrate was primarily found in the upper dermis, predominated (Table 1 and Figures 1a, b and 2a). Only a minority of CD8 þ cells ranging from o5 to 30% of the lymphocytic skin infiltrate were seen in the skin samples (Table 1).…”

“…To analyze the skin and blood samples for T-cell receptor (TCR)-g rearrangement, we used the previously published novel oligonucleotide consensus primers covering the V g 1-8 segments (V g 1 1-8 as outer and V g 2 1-8 as inner primers) 19 and the J region (JGT 1/2 , JGT 3 ), 20 and for TCR-b rearrangement, consensus primers covering the V b 1 to V b 65 segments and the primers J b FS1A/J b FS1 and J b FS2A/ J b FS2 for the J region. 21 …”

Sézary syndrome is a unique cutaneous T-cell lymphoma as identified by an expanded gene signature including diagnostic marker molecules CDO1 and DNM3

“…2 Analysis of the TCR g or b chain genes was performed using the well-established polymerase chain reaction-based GeneScan technique. 3 All patients with SS had erythroderma, peripheral lymphadenopathies, a highly elevated CD4/CD8 ratio and atypical circulating Sezary cells in the blood (Supplementary Table 1). For comparative analysis, blood samples were obtained from 10 healthy donors (4 males and 6 females, median age 57 years, ranging from 34 to 84 years).…”

“…2 Sezary cells are malignant circulating CD4 þ T cells that derive from the same T-cell clone as the malignant T cells in skin and other organs as demonstrated by T-cell receptor (TCR) gene rearrangement analysis studies. 3,4 The detection of Sezary cells in the peripheral blood is primarily based on morphological features such as cerebriform nuclei; therefore, 'Sezary cells' may be detected in healthy individuals as well as in inflammatory dermatoses. As a consequence, an arbitrary threshold has been set at 5% (20%) Sezary cells among peripheral blood mononuclear cells (peripheral blood lymphocytes) for the diagnosis of SS in the blood.…”

Clonality profile in relapsed precursor-B-ALL children by GeneScan and sequencing analyses. Consequences on minimal residual disease monitoring

“…16,17 The high-resolution PCR fragment analysis using GeneScan technique (PCR-GSA) is an accurate procedure in determining the size of PCR products and in discriminating between polyclonal and clonal DNA banding profiles. [18][19][20] In this study, we carried out complete GeneScan and sequence analyses of every Ig and TCR gene rearrangement independently found at diagnosis and relapse in a homogeneous series of 53 childhood precursor-B-ALL patients, in order to get detailed information on the evolution and the stability of potentially useful MRD-PCR targets.…”