Clonal spread of methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an international multicentre study

Perreten, Vincent; Kadlec, Kristina; Schwarz, Štefan; Andersson, Ulrika Grönlund; Finn, Maria; Greko, Christina; Moodley, Arshnee; Kania, Stephen A.; Frank, Linda A.; Bemis, David A.; Franco, Alessia; Iurescia, Manuela; Battisti, Antonio; Duim, Birgitta; Wagenaar, Jaap A.; Duijkeren, Engeline van; Weese, J. Scott; Fitzgerald, J. Ross; Rossano, Alexandra; Guardabassi, Luca

doi:10.1093/jac/dkq078

Cited by 410 publications

(548 citation statements)

References 37 publications

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“…Del total de cepas aisladas, y con la metodología utilizada, 66,7% de las aisladas desde gatos sanos correspondieron a S. intermedius, siendo la especie de SCP más frecuentemente aislada desde estos animales (32/48), concordando con lo reportado por otros autores 30,31 . De manera similar, en las muestras provenientes de gatos con dermatopatías, la especie de SCP predominante también correspondió a S. intermedius (66,7%), lo que también concuerda con lo reportado internacionalmente 4,36 .…”

Section: Discussionunclassified

“…No obstante, en los últimos años se ha identificado un número creciente de cepas de S. intermedius, S. pseudintermedius y S. schleiferi subsp. coagulans resistentes a meticilina [29][30][31][32] . Esto sugiere que los animales de compañía, como gatos y perros, pueden poseer cepas de SCP resistentes a meticilina, lo que puede suponer un problema preocupante para la medicina veterinaria, medicina humana y salud pública.…”

Section: Microbiologíaunclassified

“…Además, el incremento en la prevalencia de cepas del SIG resistentes a meticilina y su potencial zoonótico constituyen un elemento de creciente interés para la práctica clínica de animales menores en medicina veterinaria y para la salud pública 30,64 , situación que debiese enfrentarse según las indicaciones de FAO-OMS-OIE bajo el concepto de "Una Salud" 65 .…”

Section: Artículo Originalunclassified

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Detección del gen mecA en cepas de Staphylococcus coagulasa positiva aisladas desde gatos

Galarce

Muñoz

Jara

et al. 2016

Rev. chil. infectol.

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Section: Discussionunclassified

Section: Microbiologíaunclassified

Section: Artículo Originalunclassified

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Detección del gen mecA en cepas de Staphylococcus coagulasa positiva aisladas desde gatos

Galarce

Muñoz

Jara

et al. 2016

Rev. chil. infectol.

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“…It represents the most common multilocus sequence type (ST) previously reported in the United States (ST68) [6,8,42]. Bacterial colonies grown on blood agar plates were inoculated into 5ml of sterile trypticase soy broth (TSB) (BD Biosciences, San Jose, CA, USA Cat No.…”

Section: Methodsmentioning

confidence: 99%

Characterization of recombinant wild-type and nontoxigenic protein A from Staphylococcus pseudintermedius

2018

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Background: Staphylococcus pseudintermedius is an opportunistic pathogen that is the major cause of pyoderma affecting dogs. Conventional antimicrobial treatment for infections caused by this organism have failed in recent years due to widespread resistance and alternative treatment strategies are a high priority. Protein A encoded in Staphylococcus aureus by spa protects the bacterium by binding IgG and acts as a superantigen. Staphylococcus pseudintermedius possess two genes orthologous to S. aureus spa, spsP, and spsQ. Methods: SpsQ and SpsQ-M, a non-toxigenic SpsQ, were cloned and expressed as recombinant proteins and their cytotoxic effect on canine B cells was measured. The neutralizing ability of antibody raised against them in clinically healthy dogs was evaluated. Results: S. pseudintermedius SpsQ induced apoptosis of canine B cells. Specific amino acid substitutions diminished SpsQ-M binding to immunoglobulin and its super-antigenic activity, while its antigenicity was maintained. This recombinant, non-toxigenic S. pseudintermedius SpsQ stimulated the production of antibodies in dogs that specifically reacted with SpsQ and greatly diminished its cytotoxic effect on canine B cells. Conclusions: The production of neutralizing antibody suggests that attenuated, non-toxic SpsQ produced in this study is a good candidate for inclusion in a vaccine for use in the treatment and prevention of S. pseudintermedius infections.Abbreviations: SpA: Staphylococcus aureus protein A; SpsP: Staphylococcus pseudintermedius protein A; SpsQ: Staphylococcus pseudintermedius protein A; SpsQ-M: attenuated Staphylococcus pseudintermedius protein A; MRSP: methicillin resistant Staphylococcus pseudintermedius; IgA: immunoglobulin A; IgG: immunoglobulin G; IgM: immunoglobulin M; VH: variable region of immunoglobulin heavy chain; IgBD: immunoglobulin binding domains; MFI: mean fluorescent intensity; SEM: standard error of the mean; PBMC: Peripheral blood mononuclear cells; CD21: complement receptor type 2; ST: Sequence type; OD: Optical density; ORF: open reading frame; PBS: Phosphate buffered saline; Tween 20: Polyethylene glycol sorbitan monolaurate 20; HRP: horseradish peroxidase; TMB- 3,3',5,5'-Tetramethylbenzidine

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“…However, it remains unclear whether one or more clonal types of S. pseudintermedius are primarily responsible for surgical infections in dogs following orthopaedic intervention (Fazakerley et al, 2010;Paul et al, 2012;Pinchbeck et al, 2006). Despite the clonal diversity reported among S. pseudintermedius in dogs, the multidrug-resistant clonal lineage (ST71-t02-SCCmec II-III) is thought to have spread among dogs in Europe (Perreten et al, 2010). Although a comprehensive understanding of the virulence determinants of S. pseudintermedius is still limited, some of its virulence determinants such as proteins that mediate adherence to canine corneocytes, Staphylococcus intermedius exfoliative toxin (SIET), leukocidins (lukS and lukF), IP: 54.202.243.64 On: Thu, 10 May 2018 04:34:40 staphylococcal enterotoxins and biofilm production factors have been identified (Bannoehr et al, 2012;Fitzgerald, 2009;Sasaki et al, 2007;van Duijkeren et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Genotypic relatedness and characterization of Staphylococcus pseudintermedius associated with post-operative surgical infections in dogs

Diribe

Thomas

AbuOun

et al. 2015

Journal of Medical Microbiology

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Staphylococcus pseudintermedius is a commensal organism of dogs that can also be implicated in surgical site infections (SSIs) in dogs. Particularly with the recent emergence and spread of the ST71-t02-SCCmec II-III multidrug-resistant S. pseudintermedius clonal lineage (MDRSP), it is important to understand the clonal diversity of S. pseudintermedius in SSIs in dogs. The study reported here investigated the genotypic relatedness of 124 S. pseudintermedius isolates from the surgical wounds of 90 dogs admitted to a referral practice in the UK. This study also aimed to understand whether MDRSP is better adapted to survival and persistence in different environments compared with other S. pseudintermedius. Whilst no individual S. pseudintermedius clonal type was primarily responsible for S. pseudintermedius-associated SSIs in dogs, we found that MDRSP was the most represented clonal type among the isolates studied. However, we observed no difference in the level of biofilm production, susceptibility to biocides or carriage of specific virulence determinants between MDRSP and other S. pseudintermedius isolates studied. Interestingly, in the competitive fitness study, MDRSP did not outcompete any member of the other S. pseudintermedius isolates studied in each environment. Our data suggest that the determinants that promote S. pseudintermedius-associated SSIs in dogs are distributed among S. pseudintermedius as a species and are not restricted to a few clonal types. They also provide evidence to support the suggestion that MDRSP is not better adapted to survival or persistence in different environments and is no more virulent than other S. pseudintermedius isolates.

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Clonal spread of methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an international multicentre study

Cited by 410 publications

References 37 publications

Detección del gen mecA en cepas de Staphylococcus coagulasa positiva aisladas desde gatos

Detección del gen mecA en cepas de Staphylococcus coagulasa positiva aisladas desde gatos

Characterization of recombinant wild-type and nontoxigenic protein A from Staphylococcus pseudintermedius

Genotypic relatedness and characterization of Staphylococcus pseudintermedius associated with post-operative surgical infections in dogs

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