Genotypic relatedness and characterization of Staphylococcus pseudintermedius associated with post-operative surgical infections in dogs

Diribe, Onyinye; Thomas, Sarah; AbuOun, Manal; Fitzpatrick, Noel; Ragione, Roberto M. La

doi:10.1099/jmm.0.000110

Cited by 9 publications

(7 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…we selected synergistic antimicrobials, 23 2. antimicrobials that were frequently used in the management of human periprosthetic infections at the time (2012) our patient was treated, 24 3. antimicrobials that have had documented use by the calcium sulphate bead manufacturer, 25 4. and based on microbiology data from surgical site infections at our practice. 26 During the original THR procedure for the dog in this report, an intraoperative swab was taken for microbiology, which failed to culture any organisms. No septic source for the periprosthetic infection 13 months later was observed, suggestive of haematogenous bacterial spread or the presence of residual bacteria from the original procedure.…”

Section: Discussionmentioning

confidence: 93%

Single-Stage Revision of an Infected Total Hip Replacement Using Antibiotic-Impregnated Bioabsorbable Beads in a Canine Patient

Guthrie¹,

Fitzpatrick²

2019

VCOT Open

Self Cite

View full text Add to dashboard Cite

This report describes a technique and the outcome following surgical revision of a periprosthetic infected total hip replacement (THR) in a single-stage procedure with the use of antibiotic-impregnated bioabsorbable beads. A 6-year-old German Shepherd dog underwent THR 16 months previously, which subsequently became infected. The contaminated femoral and acetabular prostheses were explanted. A femoral window was used to remove the stem and cement. The endosteal surfaces of the femur and acetabulum were reamed to remove periprosthetic tissues. The femoral window was replaced and secured in position with multiple cerclage wires. A 3.5-mm locking plate was applied to the lateral aspect of the femur to prevent fracture of the proximal femoral metaphysis and trochanter. Bioabsorbable beads of calcium sulphate, impregnated with vancomycin and gentamicin, were impacted into the femoral canal followed by an uncemented femoral stem. An uncemented acetabular implant was impacted and additional antibiotic beads placed in the vicinity prior to closure. There has been no evidence of reinfection 5 years postoperatively. Analgesic and antibacterial drugs were not required during this follow-up period; activity is unrestricted with neither lameness nor pain present. Simultaneous revision of both femoral and acetabular periprosthetic infected THR implants was successfully achieved in this patient via a single-stage procedure using uncemented implants and antibiotic-impregnated bioabsorbable beads.

show abstract

Section: Discussionmentioning

confidence: 93%

Single-Stage Revision of an Infected Total Hip Replacement Using Antibiotic-Impregnated Bioabsorbable Beads in a Canine Patient

Guthrie¹,

Fitzpatrick²

2019

VCOT Open

Self Cite

View full text Add to dashboard Cite

show abstract

“…PCRs were performed to detect lukS, lukF and Panton-Valentine leukocidin genes, comparing multidrug resistant and multidrug susceptible S. pseudintermedius associated with postoperative surgical canine infections (Diribe et al 2015). No differences in carriage of these virulence factor genes, between both groups of S. pseudintermedius, were observed.…”

Section: Staphylococcus Intermedius Groupmentioning

confidence: 99%

Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus

González-Martín

Corbera

Suárez‐Bonnet

et al. 2020

Veterinary Quarterly

View full text Add to dashboard Cite

Coagulase-positive Staphylococci (CoPS) can exist as commensals in humans, companion and food-producing animals, but can cause severe or even lethal diseases. Exchange of these bacteria between humans and animals has been described. Special attention has been focused on Methicillin-Resistant Staphylococcus aureus, but other CoPS can also represent an important threat. In addition to significant antimicrobial resistance, these bacteria may carry a plethora of virulence factors-molecules that allow bacteria to establish on or within a host and increase their ability to cause disease. These virulence factors have been widely described in S. aureus but information about other species of CoPS is scarce. The aim of this paper is to review the recent literature about the virulence factors of non-aureus CoPS of animal origin. Their possible effects on human health are also described. The role and prevalence of different virulence factors including leukocidins, hemolysins, adhesins, enterotoxins, exfoliative and toxic shock syndrome toxins as well as superantigen-like proteins are addressed. The effect of these virulence factors on human health is also described. The possibility of misdiagnosis of species of CoPS has been demonstrated in human clinical samples. Prevalence of zoonotic infections could be higher than thought and medical laboratories should be aware of these other staphylococcal species. In keeping with the 'One Health' approach to animal and human disease, medical professionals, veterinarians and health workers should be aware of the risks derived from exposure to these bacteria in people in close contact with animals, including pet owners, farmers and veterinarians themselves.

show abstract

“…The efficiency and speed of LAMP technology means that tests can detect STEC infections and provide information on, for example, AMR profiles, in <15 min, directly from a swab, with excellent specificity and sensitivity (Diribe, Thomas, AbuOun, Fitzpatrick, & La Ragione, ; Diribe et al., ).…”

Section: Rapid Detection and Diagnosismentioning

confidence: 99%

Enterohaemorrhagic and other Shiga toxin-producingEscherichia coli(STEC): Where are we now regarding diagnostics and control strategies?

Newell

Ragione

2018

Transbound Emerg Dis

View full text Add to dashboard Cite

Escherichia coli comprises a highly diverse group of Gram-negative bacteria and is a common member of the intestinal microflora of humans and animals. Generally, such colonization is asymptomatic; however, some E. coli strains have evolved to become pathogenic and thus cause clinical disease in susceptible hosts. One pathotype, the Shiga toxigenic E. coli (STEC) comprising strains expressing a Shiga-like toxin is an important foodborne pathogen. A subset of STEC are the enterohaemorrhagic E. coli (EHEC), which can cause serious human disease, including haemolytic uraemic syndrome (HUS). The diagnosis of EHEC infections and the surveillance of STEC in the food chain and the environment require accurate, cost-effective and timely tests. In this review, we describe and evaluate tests now in routine use, as well as upcoming test technologies for pathogen detection, including loop-mediated isothermal amplification (LAMP) and whole-genome sequencing (WGS). We have considered the need for improved diagnostic tools in current strategies for the control and prevention of these pathogens in humans, the food chain and the environment. We conclude that although significant progress has been made, STEC still remains an important zoonotic issue worldwide. Substantial reductions in the public health burden due to this infection will require a multipronged approach, including ongoing surveillance with high-resolution diagnostic techniques currently being developed and integrated into the routine investigations of public health laboratories. However, additional research requirements may be needed before such high-resolution diagnostic tools can be used to enable the development of appropriate interventions, such as vaccines and decontamination strategies.

show abstract

Genotypic relatedness and characterization of Staphylococcus pseudintermedius associated with post-operative surgical infections in dogs

Cited by 9 publications

References 36 publications

Single-Stage Revision of an Infected Total Hip Replacement Using Antibiotic-Impregnated Bioabsorbable Beads in a Canine Patient

Single-Stage Revision of an Infected Total Hip Replacement Using Antibiotic-Impregnated Bioabsorbable Beads in a Canine Patient

Virulence factors in coagulase-positive staphylococci of veterinary interest other than Staphylococcus aureus

Enterohaemorrhagic and other Shiga toxin-producingEscherichia coli(STEC): Where are we now regarding diagnostics and control strategies?

Contact Info

Product

Resources

About