Clonal Propagation of Dwarf Raspberry (Rubus pubescens Raf.) through in vitro Axillary Shoot Proliferation

Debnath, Samir C.

doi:10.1023/b:grow.0000040110.53216.6a

Cited by 39 publications

(25 citation statements)

References 29 publications

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“…Se ha observado que varios cultivares de frambuesa responden a concentraciones que van desde 0.5 hasta 2 mg L -1 de BA en combinación con AIB o ANA, o solo zeatina (Graham et al, 1997;Palonem y Buszard, 1998;Debnath, 2004;Zawadzkay Orlikowska, 2006;Wu et al, 2009), o bien de 1 a 2 mg L -1 de tidiazuron con 0.5-1 mg L -1 de ácido 1H-indole-3-butanoico (AIB) (Swartz et al, 1990;Cousineau y Donnelly, 1991). The number of raspberry shoots produced in vitro depend largely on the concentration of cytokinins, specifically benzyladenine and on the propagated cultivar (Avitia and Rodriguez, 1984;McNicol and Graham, 1990).…”

Section: Resultados Y Discusión Formación De Brotesunclassified

“…The number of raspberry shoots produced in vitro depend largely on the concentration of cytokinins, specifically benzyladenine and on the propagated cultivar (Avitia and Rodriguez, 1984;McNicol and Graham, 1990). It has been observed that several cultivars of raspberry respond to concentrations ranging from 0.5 to 2 mg L -1 of BA in combination with IBA or IAA, or only zeatin (Graham et al, 1997;Palonem and Buszard, 1998;Debnath, 2004;Zawadzkay Orlikowska, 2006;Wu et al, 2009), or 1-2 mg L -1 of thidiazuron with 0.5-1 mg L -1 1H-indole-3-butanoic acid (IBA) (Swartz et al, 1990;Cousineau and Donnelly, 1991).…”

Section: Resultados Y Discusión Formación De Brotesmentioning

confidence: 99%

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La elongación de brotes adventicios de frambuesa (Rubus ideaus L.) es influenciada por brasinosteroides

Robres-Torres

López-Medina

Rocha-Granados

2017

Remexca

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ResumenLa técnica de cultivo in vitro fue utilizada para estudiar el efecto de la combinación de benciladenina (BA) y brasinolido (BL), dos reguladores de crecimiento vegetal, en la formación y elongación de brotes adventicios de frambuesa (Rubus idaeus L.). También se evaluó el enraizamiento de los brotes regenerados tanto en condiciones in vitro como ex vitro. Tres selecciones de frambuesa, UMC-702, UMC-706 y UMC-708, generadas de un programa de mejoramiento genético en la Facultad de Agrobiología, fueron seleccionadaspara llevar a cabo esta investigación. En la fase de multiplicación, el medio MS fue suplementado con diferentes combinaciones de bencialadenina (0 y 1.5 mg L -1 ) y brasinolido (0, 0.00001 y 0.00002 µM). La formación de brotes adventicios no fue dependiente de la combinación de los reguladores; sin embargo, la elongación de los mismos fue significativamente influenciada por el genotipo y el uso de brasinolido (BL); los mejores resultados se obtuvieron con la selección UMC-706 expuesta a 0.00002 µM de BL. El enraizamiento de los brotes adventicios fue mejor en condiciones ex vitro que in vitro, con mayor formación y elongación tanto de raíz como de los brotes; ello permitió una mejor aclimatación y adaptación de las plantas, lo que significa un ahorro tanto en tiempo como en costos de labor para la micropropagación de frambuesa. Así, este es el primer reporte del uso de brasinosteroides en frambuesa. AbstractIn vitro culture techniques have been used to study the effect of the combination of benzyladenine (BA) and brassinolide (BL), two plant growth regulators, in the formation and adventitious shoot elongation of raspberry (Rubus idaeus L.). Rooting of regenerated shoots both in vitro and ex vitro conditions were evaluated. Three selections of raspberry, UMC-702 UMC-706 and UMC-708, generated from a breeding program at the Faculty of Agrobiology, were selected to conduct this research. In the multiplication phase, the MS medium was supplemented with different combinations of benzyladenine (0 and 1.5 mg L -1 ) and brassinolide (0, 0.00001 and 0.00002 µM). Adventitious shoot formation was not dependent on the combination of regulators; however, the elongation of it was significantly influenced by genotype and use of brassinolide (BL); the best results were obtained with UMC-706 exposed to 0.00002 µM of BL. Rooting of adventitious shoots were better in ex vitro than in vitro conditions, with higher formation and elongation both root and shoots; allowing a better acclimatization and adaptation of plants, which means savings in time and labor for micro propagation of raspberry. So, this is the first report on the use of brassinosteroids in raspberry.

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Section: Resultados Y Discusión Formación De Brotesunclassified

Section: Resultados Y Discusión Formación De Brotesmentioning

confidence: 99%

La elongación de brotes adventicios de frambuesa (Rubus ideaus L.) es influenciada por brasinosteroides

Robres-Torres

López-Medina

Rocha-Granados

2017

Remexca

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“…Em outras espécies de pequenas frutas, sob condições in vitro, foram obtidas taxas de multiplicação superiores, sendo de 5,0 para morangueiro (Brahm & Oliveira, 2004) e de 6,2 para amoreira-preta (Oliveira et al, 2007). Em framboesa, Debnath (2004) obteve valor idêntico ao do presente trabalho.…”

Section: In Vitro Multiplication Rate Of Raspberry Cultivarsunclassified

“…A ausência de calos nas plantas indica adequado ajuste do protocolo de micropropagação. Ao utilizar concentrações mais elevadas de BAP e de zeatina no meio de cultura, Debnath (2004) obteve formação frequente de calos em plantas de framboeseira. No presente trabalho, mudas atípicas não foram observadas nas condições in vitro e no viveiro, o que pode ser atribuído à realização de apenas seis subcultivos de 40 dias durante a fase de multiplicação e à utilização de doses baixas de reguladores de crescimento (Oliveira et al, 2006).…”

Section: In Vitro Multiplication Rate Of Raspberry Cultivarsunclassified

Potencial de multiplicação in vitro de cultivares de framboeseira

Oliveira

Nino

2009

Rev. Bras. Frutic.

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RESUMO -Objetivou-se avaliar o potencial de multiplicação in vitro de quatro das principais cultivares de framboeseira utilizadas no Brasil: Autumn Bliss, Batum, Dorman Red e Heritage. Utilizou-se protocolo empregado em laboratórios comerciais. A desinfestação dos explantes foi realizada em soluções à base de álcool e hipoclorito de sódio; a cultura dos meristemas em meio semissólido MS com 1 mg L IN VITRO MULTIPLICATION RATE OF RASPBERRY CULTIVARSABSTRACT -The aim of this work was to evaluate the in vitro potential of multiplication of the four main raspberry cultivars used in Brazil: Autumn Bliss, Batum, Dorman Red and Heritage. A commercial micropropagation protocol was used. The explant desinfestation was carried out in solution of alcohol and sodium hypochlorite. The meristem culture was carried out in semi-solid MS medium with 1 mg L -1 BAP, 0.01 mg L -1 ANA and 0.1 mg L -1 AG 3 ; the explant multiplication in MS medium with 0.8 mg L -1 BAP and 15 mg L -1 iron sulphate; and the rooting in ½MS medium with 0.1 mg L -1 ANA, always at 25±4 o C, 20 μE m -2 s -1 and 16 hours photoperiod. Sixty meristems of each cultivar were used, evaluating the multiplication rate and the level of contamination, vitrification and oxidation during the phases of in vitro establishment (60 days), multiplication (six subcultures of 40 days) and rooting (30 days). The raspberry cultivars studied showed high genetic variability concerning in vitro multiplication rate. The estimated number of plants obtained per meristem for the described micropropagation system was of 56664 for 'Autumn Bliss', 6692 for 'Heritage', 1942 for 'Batum' and 696 for 'Dorman Red'. The quantification of this in vitro variability response is important for planning the plant production in commercial laboratories.

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“…A modified cranberry medium (Debnath and McRae 2001b) with pH adjusted 5.7 instead of 5.0 as for Vaccinium species was found suitable for culture of strawberry (Debnath 2005d(Debnath , 2006a and raspberry (Debnath 2004b). The medium contains casein hydrolysate, which might be a growth-promoting factor (Inoue and Maeda 1982).…”

Section: Culture Mediamentioning

confidence: 99%

Strategies to propagate Vaccinium nuclear stocks for the Canadian berry industry

Debnath¹

2007

Can. J. Plant Sci.

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Debnath, S. C. 2007. Strategies to propagate Vaccinium nuclear stocks for the Canadian berry industry. Can. J. Plant Sci. 87: 911-922. Vacinium fruits are genetically heterozygous species characterized as "not coming true from seed". Conventional methods for vegetative propagation of these species, although successful, are slow and labour-intensive, and few propagules can be produced from one plant of a selected clone or hybrid. Micropropagation techniques are important for clonal multiplication, germplasm improvement and gene conservation of Vaccinium fruits cultivated in Canada including blueberries, cranberries and lingonberries. In vitro propagation of these species using axillary bud proliferation and adventitious shoot regeneration has been investigated in a number of studies. Morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, and this dependence is genotype specific. The paper presents the progress in-depth of various aspects of the in vitro culture of Canadian Vaccinium species for their commercial production. Also discussed are techniques for clone rejuvenation and plant tissue culture for mass propagation of Canadian Vaccinium nuclear stocks. Les techniques de multiplication végétative in vitro revê-tent une grande importance pour la multiplication des clones, l'amélioration du matériel génétique et la conservation des gènes des fruits du genre Vaccinium cultivés au Canada, notamment le bleuet, la canneberge et l'airelle vigne-d'Ida. Diverses études se sont penchées sur la multiplication in vitro de ces espèces par prolifération des bourgeons et régénération des pousses adventives. La morphogénèse semble considérablement dépendre des régulateurs de croissance de la plante et du milieu de culture, une dépen-dance propre au génotype. L'article fait le point des progrès réalisés au niveau des divers aspects de la culture in vitro des espèces canadiennes du genre Vaccinium en vue de leur culture commerciale. On y présente aussi les techniques de rajeunissement des clones et de culture tissulaire pour la multiplication massive du matériel génétique des espèces canadiennes de Vaccinium.

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Clonal Propagation of Dwarf Raspberry (Rubus pubescens Raf.) through in vitro Axillary Shoot Proliferation

Cited by 39 publications

References 29 publications

La elongación de brotes adventicios de frambuesa (Rubus ideaus L.) es influenciada por brasinosteroides

La elongación de brotes adventicios de frambuesa (Rubus ideaus L.) es influenciada por brasinosteroides

Potencial de multiplicação in vitro de cultivares de framboeseira

Strategies to propagate Vaccinium nuclear stocks for the Canadian berry industry

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