Clonal nuture of fibroblast colonies formed by bone marrow stromal cells in culture

Latsinik, N. V.; Grosheva, A. G.; Narovlyanskii, A. N.; Pavlenko, R. G.; Fridenshtein, A. Ya.

doi:10.1007/bf00840577

Cited by 6 publications

(5 citation statements)

References 5 publications

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“…The clonal nature of fibroblast colonies formed by MMSCs in tissue cultures [4][5][6][7] allows to study the changes in their numbers judging by the increase or decrease in the effectiveness of colony formation, i.e. by the ratio of the number of formed colonies to the number of explanted cells.…”

Section: Resultsmentioning

confidence: 99%

Proline-Rich Hypothalamic Polypeptide Has Opposite Effects on the Proliferation of Human Normal Bone Marrow Stromal Cells and Human Giant-cell Tumour Stromal Cells

et al. 2010

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In the present study we carried out experiments in vitro and in vivo and investigated the effect of proline-rich polypeptide (PRP) on the proliferation and effectiveness of colony formation of MMSCs in vitro. Various routes and doses of PRP administration to rats increased the number of MMSCs in bone marrow and spleen. Our research revealed opposite effects of PRP on the proliferation of bone marrow stromal cells obtained from normal humans and stromal cells isolated from a human giant-cell tumour.

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Section: Resultsmentioning

confidence: 99%

Proline-Rich Hypothalamic Polypeptide Has Opposite Effects on the Proliferation of Human Normal Bone Marrow Stromal Cells and Human Giant-cell Tumour Stromal Cells

et al. 2010

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“…After that, the analysis of the colonies was carried out. The colony forming effectiveness of the fibroblasts (ECO-f) was determined according to Fridenshtein’s equation for stromal progenitor cells: ECO-f = a ratio between the number of pre-formed colonies and the number of explanted cells multiplied by 100% [50,51]. The only counted colonies include more than 20 cells of the total number of explanted cells.…”

Section: Methodsmentioning

confidence: 99%

Spontaneous γH2AX foci in human dermal fibroblasts in relation to proliferation activity and aging

Zorin

Grekhova

Pustovalova

et al. 2019

Aging

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We assessed the effects of donor age on clonogenicity, proliferative potential, and spontaneous γH2AX foci in the proliferating (Ki67 +) and senescent (SA β-gal +) cultures of skin fibroblasts isolated from 34 donors of different age (23-82 years). Here, we demonstrated that neither the colony forming effectiveness of proliferating (Ki67+) fraction of the fibroblasts nor the average number of γH2AX foci of the same fraction does not depend on the age of the donor. The correlation between the number of γH2AX foci and the donor’s age was reliable in quiescent (Ki67-) cells. The average number of γH2AX foci in quiescent fibroblasts of donors older than 68 years was about two times higher than in the same cells of up to 30 years old donors. The number of γH2AX foci demonstrated a statistically significant positive correlation with the fraction of proliferating cells in fibroblast cultures. On average, proliferating cells have twice as many the γH2AX foci in comparison with the quiescent cells. Within a population of proliferating (Ki67+) cells, the degree of senescence correlated with a relative declining of constitutive γH2AX foci number, whereas in the population of quiescent (Ki67-) cells, it was proportional to augmenting the number of the γH2AX foci. Our data on a statistically significant (p=0.001) correlation between the age of the donor and the number of constitutive γH2AX foci in quiescent cells, could point out the ongoing DNA-damage response due in the maintenance of the senescent state of cells.

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“…These CFU-F adhered to tissue culture plastic and formed both cartilage and bone when placed in diffusion chambers and implanted into animals Mardon et al, 1987]. When the same cells were placed in culture, they also displayed evidence of osteogenesis [Latzinik et al, 1987;Owen et al, 1987]. It is now recognized that the bone marrow stroma is a major reservoir of these mesenchymal stem cells, which can be induced to differentiate not only into osteoblasts and chondrocytes, but also adipocytes, neural cells and fibroblastic cells that support hematopoiesis [Grigoriadis et al, 1988;Aubin, 1998;Dennis et al, 1999;Kopen et al, 1999;Muraglia et al, 2000].…”

Section: Introductionmentioning

confidence: 99%

Different Effects of BMP-2 on Marrow Stromal Cells from Human and Rat Bone

Osyczka

Diefenderfer

Bhargave

et al. 2004

Cells Tissues Organs

149

109

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Bone morphogenetic proteins (BMPs) promote the differentiation of osteoprogenitor cells, and also induce osteogenesis in bone marrow stromal cells (MSC) from rats and mice. However, compared to results with animal models, BMPs are relatively inefficient in inducing human MSC to undergo osteogenesis, and are much less effective in promoting bone formation in human clinical trials. Previous studies indicated that, while human MSC respond to dexamethasone with elevated levels of the osteoblast marker alkaline phosphatase, most isolates of human MSC fail to show alkaline phosphatase induction in response to BMP-2, BMP-4, or BMP-7. Several other genes known to be induced by BMPs are appropriately regulated; thus, human MSC are capable of some BMP-activated signaling. Analysis of the BMP receptors ALK-3 and ALK-6 indicated that, although ALK-6 mRNA was not expressed in human MSC, overexpressing a constitutively active ALK-6 receptor did not induce elevated alkaline phosphatase. Real-time RT-PCR was used to investigate expression of several osteoblast-related transcription factors in MSC after 6 days’ exposure to BMP2 or dexamethasone. Msx-2, a transcription factor that has been reported to inhibit differentiation of osteoprogenitor cells, showed 10-fold elevation in BMP-2-treated human MSC, but not in BMP-2-treated rat MSC. Overexpression of Msx-2 in human and rat MSC, however, did not alter alkaline phosphatase levels, which suggests that absence of BMP-stimulated alkaline phosphatase was not caused by the BMP-2-induced increase in Msx-2. Although Runx2 isoforms have been implicated in control of osteoblast differentiation, levels of this transcription factor were unaffected by BMP treatment. Expression of the FKHR transcription factor, which has been reported to regulate alkaline phosphatase transcription in mouse cells, showed a modest increase in response to BMP-2, but a much greater increase in dexamethasone-treated cells. We propose that BMP regulation of the bone/liver/kidney alkaline phosphatase gene is indirect, requiring expression of new transcription factor(s) that behave differently in rodent and human MSC.

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Clonal nuture of fibroblast colonies formed by bone marrow stromal cells in culture

Cited by 6 publications

References 5 publications

Proline-Rich Hypothalamic Polypeptide Has Opposite Effects on the Proliferation of Human Normal Bone Marrow Stromal Cells and Human Giant-cell Tumour Stromal Cells

Proline-Rich Hypothalamic Polypeptide Has Opposite Effects on the Proliferation of Human Normal Bone Marrow Stromal Cells and Human Giant-cell Tumour Stromal Cells

Spontaneous γH2AX foci in human dermal fibroblasts in relation to proliferation activity and aging

Different Effects of BMP-2 on Marrow Stromal Cells from Human and Rat Bone

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