Clonal Micropropagation, Rhizogenesis and Adaptive Capacity of Certain Rose (Rosa L.) Variety Explants

Abstract

This article presents research on the inclusion of the biotechnological link in rose breeding. Roses are heterozygous plants and give splitting when propagated by seed. Therefore, clonal micropropagation has been included in the research to reproduce and preserve valuable genotypes and new starting material. The aim of this study was to select the type of auxin and its optimal concentrations for better Rhizogenesis of micro-sprouts of selected rose cultivars, and to select the suitable substrate for more effective adaptation of regenerant plants. For the introduction of explants into culture, cultivation and preparation of nutrient media, the traditional methods adopted in tissue culture work were applied. For rooting of well-formed micro-sprouts, liquid nutrient medium with a halved content of macro- and microsalts and sucrose was used. In addition, different concentrations of 3-indolyl butyric acid and β-indolyl-3-acetic acid were added to the medium.Based on the findings of the study, it is recommended to use indolyl butyric acid at concentrations of 0.5 mg/l and 1.0 mg/l for clonal micropropagation of roses, since at these concentrations, the rooting frequency indices by varieties were the highest. When adapting the plant material, it is better to use a substrate consisting of peat, soddy soil and perlite in a ratio of 1:1:1. The best results in the studies were noted in the varieties Alan Titchmarsh, Coral Surprise and Tchaikovski.

The Influence of Cytokinins on the Growth and Development of Patio Group Roses in «in Vitro» Conditions

“…As explants for introduction into in vitro culture, buds (sockets) 0.5-0.8 cm in size were used, taken from donor plants (grown in closed soil) with a typical phenotype without damage by pests and viruses. Buds were selected during the vegetation period, in the phase of active vegetation and in a state of rest [3,6].…”