Clonal micropropagation in vitro of essential oil plants of the family Lamiaceae Lindl.

Abstract

The relevance of research on the development of biotechnology for clonal micropropagation of plants of the family Lamiaceae Lindl. is determined by the need for mass production of healthy, pure-grade planting material for the establishment of industrial plantations and the expansion of areas of essential oil crops in Ukraine. The aim of the research was to develop biotechnological methods of clonal micropropagation of essential oil plants of the Lamiaceae family – Lavandula angustifolia Mill., Mentha x piperita L., Salvia officinalis L. and Monarda fistulosa L. The task of the research was to select optimal conditions for effective cultivation of plants of the Lamiaceae family at four stages of clonal micropropagation. The main methods of research: laboratory, field, analytical, mathematical and statistical. The optimal nutrient media for the induction of in vitro morphogenesis and the multiplication stage were determined based on the basic medium of Murashige and Skoog: for L. angustifolia supplemented with kinetin (1.0 mg/l) and gibberellic acid (1.0 mg/l), for M. x piperita – with 6-benzylaminopurine (1.0 mg/l) and gibberellic acid (0.1 mg/l), for S. officinalis – with 6-benzylaminopurine (1.0 mg/l) and IOLK (0.5 mg/l ), for M. fistulosa – 6-benzylaminopurine (1.0 mg/l) and β-indolyl-3-oleic acid (0.1 mg/l). At the stage of multiplication, it is advisable to carry out seven to eight cycles of cultivation. At the stage of rooting of microshoots, the most effective for all studied plant species was determined to be the Murashige and Skuga nutrient medium with a halved concentration of components, supplemented with β-indolyl-3-oleic acid (0.5 mg/l) and β-indolyl-3-acetic acid ( 0.5 mg/l). The optimal substrate for plant adaptation to in vivo conditions is peat: perlite in a volume ratio of 3:1. Incorporation of the developed biotechnology of clonal micropropagation into the seeding system of essential oil crops of the Lamiaceae family will allow to quickly obtain healthy pure-bred planting material and introduce new productive varieties into production