Clonal identity of <i>Candida albicans</i> in the oral cavity and the gastrointestinal tract of pre‐school children

Hossain, Hamid; Ansari, Franziska; Schulz‐Weidner, Nelly; Wetzel, Willi-Eckhard; Chakraborty, Trinad; Domann, Eugen

doi:10.1034/j.1399-302x.2003.00086.x

Cited by 50 publications

(44 citation statements)

References 26 publications

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“…In this study more than one C. albicans colony was selected and stored for fingerprinting and it was unlikely that it could be attributed to chance that 12 of the 13 paired isolates were identical. Demonstration of the same genetic subtype from a particular patient's oral soft tissue also colonising that patient's carious dentine, is similar to the findings of another study in which identical C. albicans genetic subgroups were demonstrated to colonise two different anatomical sites [39].…”

Section: Discussionsupporting

confidence: 75%

Molecular and Histological Association Between Candida albicans from Oral Soft Tissue and Carious Dentine of HIV-Positive Children

Blignaut

Heerden

2015

Mycopathologia

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Abstractand caries are frequently investigated among healthy and immunosuppressed individuals. The objective of this study was to demonstrate the presence of on both oral soft and hard tissue and to investigate, at molecular level, the genetic subtype of the organism from the two oral sites. Tongue swabs and dentine scrapings from 362 HIV-positive children, referred for extraction of carious primary teeth, were cultured on CHROMagar and identified to species level with ID32C. Histological staining of extracted carious teeth was also done. In patients with positive cultures from both the tongue and carious dentine, DNA fingerprinting of such paired isolates were performed, using Southern blot hybridisation with the Ca3 probe. Yeasts were cultured from the tongue of 151 (41.7%) individuals and 57 (37.7%) simultaneously yielded positive cultures from carious dentine. Nine different yeast spp. were identified from the tongue using the ID32C commercial system but was the only species recovered from carious dentine and histological investigation demonstrated fungal elements penetrated into the dentine and not limited to superficial debris on the floor of the cavity. Twelve of 13 paired isolates of revealed identical fingerprinting patterns. The findings from this study demonstrated that in a particular individual, the same genetic subtype of was capable of colonizing both oral soft tissue and carious dentine. This renders carious teeth a constant source, or reservoir, of potentially infectious agents and, particularly among immunosuppressed individuals, should therefore not be left unattended.

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Section: Discussionsupporting

confidence: 75%

Molecular and Histological Association Between Candida albicans from Oral Soft Tissue and Carious Dentine of HIV-Positive Children

Blignaut

Heerden

2015

Mycopathologia

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“…Implementation of a preventive oral hygiene program was effective in reducing oral colonization by Candida in the elderly [56]. Analysis of saliva, dental plaque, carious teeth, and feces for identification of C. albicans revealed the presence of identical strains in the oral cavity and feces of preschool children, indicating a significant correlation between oral and gastrointestinal C. albicans colonization [58]. These data are in concordance with previous studies demonstrating the relatedness of Candida strains derived from oral surfaces and from distant parts of the body, proposing that Candida isolated from oral lesions could serve as a reservoir for C. albicans colonization and invasion of nonoral tissues, causing dissemination to the GI tract, skin, and lungs [59].…”

Section: Host Oral Hygienementioning

confidence: 97%

Geographic Trends in Invasive Candidiasis

Nishikaku

Melo

Colombo

2010

Curr Fungal Infect Rep

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Candida species are one of the most important causes of bloodstream infection (BSI) in tertiary-care hospitals worldwide. The incidence of candidemia and the Candida species causing these infections may vary geographically. Although C. albicans remains the species most commonly isolated, there is clear evidence showing increasing rates of BSI caused by Candida non-albicans species around the world. C. glabrata is the second most common cause of candidemia in North America, but it is less frequently isolated in Latin America. On the other hand, C. parapsilosis complex represents the second or the third most common species found in Latin American and Iberian countries, while C. tropicalis has emerged as a frequent agent of BSI in Latin America and Asia-Pacific regions. In this context, a complex set of clinical aspects and biologic factors may contribute to the geographic trends in the epidemiology of candidemia.

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“…albicans is a persistent [Starr et al, 2002] widespread member of the oral microbiota of increased abundance and diversity in children with high caries [Hossain et al, 2003;Mardegan et al, 2006;Moalic et al, 2001]. It is highly acidogenic and in one study released calcium from hydroxyapatite at 20 times the rate of S. mutans [Nikawa et al, 2002].…”

Section: Discussionmentioning

confidence: 99%

Microbiota of Plaque Microcosm Biofilms: Effect of Three Times Daily Sucrose Pulses in Different Simulated Oral Environments

Sissons

Anderson²,

Wong³

et al. 2007

Caries Res

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Aim: To explore the Ecological Plaque Hypothesis for dental caries. To test modification of the microbiota of dental plaque microcosm biofilms by sucrose pulsing during growth in two different simulated oral fluids, and with a urea-induced plaque pH elevation. Methods: Plaque microcosm biofilms were cultured in an ‘artificial mouth’ with and without 6-min 5% w/v sucrose pulses every 8 h in an environment of continuously supplied saliva-like defined medium with mucin (DMM), or basal medium mucin (BMM, a high-peptone-yeast extract oral fluid analogue), and also in DMM + 10 mmol/l urea, with sucrose pulsing. Forty plaque species were quantified by checkerboard DNA:DNA hybridization analysis. Results: Sucrose pulsing extended rapid plaque growth in DMM and BMM, inducing major microbiota changes in DMM but not in BMM. In DMM, some streptococci and lactobacilli were unaffected while others implicated in caries, together with Candida albicans and Capnocytophaga gingivalis, increased. Aerobic, microaerophilic and major anaerobic species decreased. Elevation of the pH_max from 6.4 to 7.0 had almost no effect on the microbiota. BMM plaques were distinct from DMM plaques with particularly low levels of Candida albicans and Actinomyces. Conclusions: Modest sucrose exposure in a saliva-like environment causes profound changes in the developmental self-organization of plaque microcosms, supporting the Ecological Plaque Hypothesis. Nevertheless, there is significant stability in microbial composition with varying pH near neutrality. Increases in levels of specific bacteria in response to sucrose could be characteristic of organisms particularly important in caries.

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Clonal identity of Candida albicans in the oral cavity and the gastrointestinal tract of pre‐school children

Cited by 50 publications

References 26 publications

Molecular and Histological Association Between Candida albicans from Oral Soft Tissue and Carious Dentine of HIV-Positive Children

Molecular and Histological Association Between Candida albicans from Oral Soft Tissue and Carious Dentine of HIV-Positive Children

Geographic Trends in Invasive Candidiasis

Microbiota of Plaque Microcosm Biofilms: Effect of Three Times Daily Sucrose Pulses in Different Simulated Oral Environments

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