Clonal diversity of M. tuberculosis isolated in a sea port city in Brazil

Silva, Ana Bárbara Scholante; Groll, Andréa von; Felix, Carolina Rodrigues; Conceição, Fabrı́cio R.; Spies, Fernanda Sá; Scaini, Carlos James; Rossetti, Maria Lúcia Rosa; Borsuk, Sibele; Dellagostin, Odir A.; Silva, Pedro Eduardo Almeida da

doi:10.1016/j.tube.2009.05.009

Cited by 13 publications

(7 citation statements)

References 21 publications

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“…The MIRU technique generated 93 independent genotypes and did not create any groups with 100% similarity, corroborating another study [20] in which it was shown that spoligotyping had a lower discriminatory power than MIRU. VNTR (minisatellites) are free of selective or functional pressure, so that MIRU profiles can be stable for up to 18 months of consecutive isolation [16].…”

Section: Discussionsupporting

confidence: 84%

“…The preponderance of these M. tuberculosis families in São Paulo city and Lisbon is probably due to the close relationship between Brazil and Portugal, especially between the two cities. In Brazil, studies carried out in Rio Grande do Sul [20] and in Maringa (PR) [21] corroborate the prevalence of these families. The families LAM and T and also Haarlem are most frequently found in Africa, South America and Central Europe [12].…”

Section: Discussionmentioning

confidence: 87%

“…However, locus 31 did not show high variation (ranging from alleles 1 to 5). In another study [20], loci 10, 23, 26 and 40 also showed the greatest discriminatory power.…”

Section: Discussionmentioning

confidence: 95%

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Characterization of the genetic diversity of Mycobacterium tuberculosis in São Paulo city, Brazil

Mendes

Melo²,

Santos

et al. 2011

BMC Res Notes

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BackgroundTuberculosis is a major health problem in São Paulo, Brazil, which is the most populous and one of the most cosmopolitan cities in South America. To characterize the genetic diversity of Mycobacterium tuberculosis in the population of this city, the genotyping techniques of spoligotyping and MIRU were applied to 93 isolates collected in two consecutive years from 93 different tuberculosis patients residing in São Paulo city and attending the Clemente Ferreira Institute (the reference clinic for the treatment of tuberculosis).FindingsSpoligotyping generated 53 different spoligotype patterns. Fifty-one isolates (54.8%) were grouped into 13 spoligotyping clusters. Seventy- two strains (77.4%) showed spoligotypes described in the international databases (SpolDB4, SITVIT), and 21 (22.6%) showed unidentified patterns. The most frequent spoligotype families were Latin American Mediterranean (LAM) (26 isolates), followed by the T family (24 isolates) and Haarlem (H) (11 isolates), which together accounted for 65.4% of all the isolates. These three families represent the major genotypes found in Africa, Central America, South America and Europe. Six Spoligo-International-types (designated SITs by the database) comprised 51.8% (37/72) of all the identified spoligotypes (SIT53, SIT50, SIT42, SIT60, SIT17 and SIT1). Other SITs found in this study indicated the great genetic diversity of M. tuberculosis, reflecting the remarkable ethnic diversity of São Paulo city inhabitants. The MIRU technique was more discriminatory and did not identify any genetic clusters with 100% similarity among the 93 isolates. The allelic analysis showed that MIRU loci 26, 40, 23 and 10 were the most discriminatory. When MIRU and spoligotyping techniques were combined, all isolates grouped in the 13 spoligotyping clusters were separated.ConclusionsOur data indicated the genomic stability of over 50% of spoligotypes identified in São Paulo and the great genetic diversity of M. tuberculosis isolates in the remaining SITs, reflecting the large ethnic mix of the São Paulo city inhabitants. The results also indicated that in this city, M. tuberculosis isolates acquired drug resistance independently of genotype and that resistance was more dependent on the selective pressure of treatment failure and the environmental circumstances of patients.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 87%

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Characterization of the genetic diversity of Mycobacterium tuberculosis in São Paulo city, Brazil

Mendes

Melo²,

Santos

et al. 2011

BMC Res Notes

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“…In this study, the genotypic diversity and drug resistance of M. tuberculosis strains in Northeast Brazil, and also the geographic distribution of the study sample, were characterized. It was observed that the spoligopatterns family distribution was similar to that reported in South America, 10 , 20 , 21 , 22 , 23 where the LAM and H lineages were the most prevalent. It was also found that 41 (35.7%) isolates that did not match any of the shared types present in SpolDB4 database.…”

Section: Discussionsupporting

confidence: 78%

Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil

Luiz

Suffys

et al. 2013

The Brazilian Journal of Infectious Diseases

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“… 14 Although this high rate of clustering suggests recent transmission, which might have occurred within the prison facility, the lack of studies of isolates obtained from the local extramural population is a limiting factor for the present analysis. 17 , 18 …”

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confidence: 99%

Recent transmission of drug-resistant Mycobacterium tuberculosis in a prison population in southern Brazil

et al. 2016

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We conducted a cross-sectional, retrospective study, characterized by classical and molecular epidemiology, involving M. tuberculosis isolates from a regional prison in southern Brazil. Between January of 2011 and August of 2014, 379 prisoners underwent sputum smear microscopy and culture; 53 (13.9%) were diagnosed with active tuberculosis. Of those, 8 (22.9%) presented with isoniazid-resistant tuberculosis. Strain genotyping was carried out by 15-locus mycobacterial interspersed repetitive unit-variable-number tandem-repeat analysis; 68.6% of the patients were distributed into five clusters, and 87.5% of the resistant cases were in the same cluster. The frequency of drug-resistant tuberculosis cases and the rate of recent transmission were high. Our data suggest the need to implement an effective tuberculosis control program within the prison system.

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Clonal diversity of M. tuberculosis isolated in a sea port city in Brazil

Cited by 13 publications

References 21 publications

Characterization of the genetic diversity of Mycobacterium tuberculosis in São Paulo city, Brazil

Characterization of the genetic diversity of Mycobacterium tuberculosis in São Paulo city, Brazil

Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil

Recent transmission of drug-resistant Mycobacterium tuberculosis in a prison population in southern Brazil

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