Clonal composition of human multipotent mesenchymal stromal cells

Bigildeev, Alexey; Zhironkina, Oxana A.; Shipounova, Irina N.; Sats, Natalia; Kotyashova, Svetlana Y.; Ni, Drize

doi:10.1016/j.exphem.2012.06.006

Cited by 15 publications

(10 citation statements)

References 26 publications

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“…Obviously, the MMSC population is composed of more mature stromal precursor cells. [45] At the gene expression level, we observed a prolonged downregulation of the PDGF pathway (decreased REL of PDGFRB and PDGFRA in AML-and ALL-derived MMSCs, respectively) and FGF2 pathway (the REL of FGFR1 was decreased in both AML and ALL MMSC cultures). MMSCs derived from AML patients showed a decrease in CSF1 and FGFR1 expression before allo-HSCT.…”

Section: Discussionmentioning

confidence: 90%

Alterations of the bone marrow stromal microenvironment in adult patients with acute myeloid and lymphoblastic leukemias before and after allogeneic hematopoietic stem cell transplantation

Shipounova

Petinati

Bigildeev

et al. 2016

Leukemia & Lymphoma

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Bone marrow (BM) derived adult multipotent mesenchymal stromal cells (MMSCs) and fibroblast colony-forming units (CFU-Fs) of 20 patients with acute myeloid leukemia (AML) and 15 patients with acute lymphoblastic leukemia (ALL) before and during 1 year after receiving allogeneic hematopoietic stem cell transplantation (allo-HSCT) were studied. The growth characteristics of MMSCs of all patients before allo-HSCT were not altered; however, relative expression level (REL) of some genes in MMSCs, but not in CFU-Fs, from AML and ALL patients significantly changed. After allo-HSCT, CFU-F concentration and MMSC production were significantly decreased for 1 year; REL of several genes in MMSCs and CFU-F-derived colonies were also significantly downregulated. Thus, chemotherapy that was used for induction of remission did not impair the function of stromal precursors, but gene expression levels were altered. Allo-HSCT conditioning regimens significantly damaged MMSCs and CFU-Fs, and the effect lasted for at least 1 year.

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Section: Discussionmentioning

confidence: 90%

Alterations of the bone marrow stromal microenvironment in adult patients with acute myeloid and lymphoblastic leukemias before and after allogeneic hematopoietic stem cell transplantation

Shipounova

Petinati

Bigildeev

et al. 2016

Leukemia & Lymphoma

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“…Finding the specific markers for effective MSC samples would provide more efficient prophylaxis. The MSC populations are heterogeneous and vary between individual donors as well as among cells in the sample . Changes in the MSC growth capacity were previously shown to correlate with colony‐forming efficiency and telomere length for sex‐ and age‐matched donors .…”

Section: Discussionmentioning

confidence: 99%

“…In this model, a decrease in the expression level of the receptor for the basic fibroblast growth factor (FGFR1) combined with an increase in the expression levels of differentiation‐related (PPARG) and aging‐related (IGF1) genes indicated that MSCs that failed to prevent aGvHD primarily consisted of mature cells. The MSC population has been shown to be heterogeneous and consist of cells of varying maturity . The growth capacity of MSCs depends on the expression of either FGF2 or FGFR.…”

Section: Discussionmentioning

confidence: 99%

Analysis of multipotent mesenchymal stromal cells used for acute graft‐versus‐host disease prophylaxis

Кузьмина

Petinati

Shipounova

et al. 2015

European J of Haematology

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“…The same lot of bFGF was used for all cloning experi ments to ensure comparability of results. The number of mitoses performed by CFU Fs was calculated as described [25]. Colonies from wells of 96 well plates were treated with trypsin and suspended in culture medium.…”

Section: Methodsmentioning

confidence: 99%

Investigation of the mesenchymal stem cell compartment by means of a lentiviral barcode library

Bigildeev

Cornils

Aranyossy

et al. 2016

Biochemistry Moscow

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The hematopoietic bone marrow microenvironment is formed by proliferation and differentiation of mesenchymal stem cells (MSCs). The MSC compartment has been less studied than the hematopoietic stem cell compartment. To characterize the structure of the MSC compartment, it is necessary to trace the fate of distinct mesenchymal cells. To do so, mesenchymal progenitors need to be marked at the single-cell level. A method for individual marking of normal and cancer stem cells based on genetic "barcodes" has been developed for the last 10 years. Such approach has not yet been applied to MSCs. The aim of this study was to evaluate the possibility of using such barcoding strategy to mark MSCs and their descendants, colony-forming units of fibroblasts (CFU-Fs). Adherent cell layers (ACLs) of murine long-term bone marrow cultures (LTBMCs) were transduced with a lentiviral library with barcodes consisting of 32 + 3 degenerate nucleotides. Infected ACLs were suspended, and CFU-F derived clones were obtained. DNA was isolated from each individual colony, and barcodes were analyzed in marked CFU-F-derived colonies by means of conventional polymerase chain reaction and Sanger sequencing. Barcodes were identified in 154 marked colonies. All barcodes appeared to be unique: there were no two distinct colonies bearing the same barcode. It was shown that ACLs included CFU-Fs with different proliferative potential. MSCs are located higher in the hierarchy of mesenchymal progenitors than CFU-Fs, so the presented data indicate that MSCs proliferate rarely in LTBMCs. A method of stable individual marking and comparing the markers in mesenchymal progenitor cells has been developed in this work. We show for the first time that a barcoded library of lentiviruses is an effective tool for studying stromal progenitor cells.

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Clonal composition of human multipotent mesenchymal stromal cells

Cited by 15 publications

References 26 publications

Alterations of the bone marrow stromal microenvironment in adult patients with acute myeloid and lymphoblastic leukemias before and after allogeneic hematopoietic stem cell transplantation

Alterations of the bone marrow stromal microenvironment in adult patients with acute myeloid and lymphoblastic leukemias before and after allogeneic hematopoietic stem cell transplantation

Analysis of multipotent mesenchymal stromal cells used for acute graft‐versus‐host disease prophylaxis

Investigation of the mesenchymal stem cell compartment by means of a lentiviral barcode library

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