Clonal bamboo production based on in vitro culture

Abstract

Bamboo species are an alternative for the composition of forest plantations. However, their potential has not been explored due to the hard time in producing large-scale clonal plants. Thus, the aim this work was to evaluate the in vitro establishment, bud multiplication and ex vitro rooting of Bambusa vulgaris. The first experiment tested different systemic and contact fungicide solutions, based on exposure time, during the establishment phase. Established explants were subjected to evaluation of residual fungicide effect on subcultures during the multiplication and elongation phases. The second experiment evaluated the influence of activated carbon on ex vitro survival and on adventitious rooting. Explant immersion in liquid culture medium added with 1.0 mL of fungicide for 120 hours has favored the in vitro establishment and reduced fungal contamination. On the other hand, it favored the shoot emission of shoots per explant during the multiplication phase. Both rooting induction culture medium and mini-incubator system use were effective in enabling adventitious root formation. The presence of activated carbon in the rooting induction culture medium resulted in a higher clonal plant survival rate.

WITHDRAWN: Reduction in Microbial Manifestation on In Vitro Culture of Eucalyptus Microcorys

“…lutea (Witt.) Maheswari [38] and Bambusa vulgaris [27]. Thus, given the importance of culture medium asepsis and the losses entailed by microbial manifestation in in vitro cultures, we aimed to evaluate the survival and reduction in microbial manifestation in Eucalyptus microcorys explants cultivated in vitro through the addition of sodium hypochlorite and streptomycin to the culture medium.…”

Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

“…Fungal solution (1 mg L -1 Captan, Orthocide 500®) was applied 48 h before shoot collection. Leaf sheaths of the shoot were removed and the remaining lignified tissues were scraped (Furlan et al, 2018;Ribeiro et al, 2020). Shoots were washed with sterile deionized water and neutral-pH detergent, and reduced to the nodal segment (i.e., explant) measuring 2.0 cm of length with one axillary bud, which was immersed in a solution with sodium hypochlorite (NaClO) (deionized water:sodium hypochlorite, v/v, 1.00-1.25% of active chlorine) for 10 minutes.…”

“…Identification of the pith, pericycle, aerenchyma and the presence of root hairs (Figures 7B) was possible from observing the adventitious roots. Root hairs are common in adventitious bamboo roots and facilitate the absorption of solutes by the plant (Furlan et al, 2018;Ribeiro et al, 2020). In the stem, in the portion above the node and the root-stem transition region (Figures 7C-D), there was a reduction in the cells and the emergence of a central void, which characterise the hollow region of the bamboo.…”

“…The production of clonal bamboo plants needs technological advances to effectively contribute to the encouragement of their cultivation aimed at the production of sustainable biomass. The bamboo species presents variations in seed production (Ramanayake et al, 2001;Vengala et al, 2008), a fact that limits the production of plants by seeds, and thus, vegetative propagation is a significant alternative for plant multiplication (Furlan et al, 2018;Ribeiro et al, 2016;Ribeiro et al, 2020;Konzen et al, 2021a). Although frequent, bamboo vegetative propagation by traditional methods, such as the breaking up of clumps, still cannot meet the growing demands for seedlings and the expectations generated with the increase in product diversification (Akinlabi et al, 2017;Rajput et al 2020).…”

“…The most frequent ones are endophytic organism contamination, hyperhydricity, oxidation of the culture medium due to the release of phenolic substances, unstable multiplication rate and rooting difficulty (Ray and Ali, 2017;Sandhu et al, 2018). For minimising such challenges, researchers have conducted studies that test new asepsis techniques, new culture medium, exogenous applications of plant growth regulators and factors that promote events such as shoot induction and adventitious rooting (Furlan et al, 2018;Sandhu et al, 2018;Lin et al, 2019;Ribeiro et al, 2020;Silveira et al, 2020;Konzen et al, 2021a).…”