Clofibrate‐inducible, 28‐kDa peroxisomal integral membrane protein is encoded by PEX11

Abstract

We cloned a human PEX11 cDNA by expressed sequence tag homology search using yeast Candida boidinii PEX11, followed by screening of human liver cDNA library. PEX11 encoded a peroxisomal protein Pex11p comprising 247 amino acids, with two transmembrane segments and a dilysine motif at the C-terminus. Pex11p comigrated in SDS-PAGE with a 28-kDa peroxisomal integral membrane protein (PMP28) isolated from the liver of clofibrate-treated rats and was crossreactive to anti-PMP28 antibody, thereby indicating PEX11 to encode PMP28. Pex11p exposes both N-and Cterminal parts to the cytosol. PEX11 was not responsible for ten complementation groups of human peroxisome deficiency disorders.z 1998 Federation of European Biochemical Societies.

Light Induces Peroxisome Proliferation in Arabidopsis Seedlings through the Photoreceptor Phytochrome A, the Transcription Factor HY5 HOMOLOG, and the Peroxisomal Protein PEROXIN11b

“…For example, PEX11 in yeast is up-regulated by oleic acid (Erdmann and Blobel, 1995;Marshall et al, 1995). One of the three mouse PEX11 isoforms, PEX11a, is induced by peroxisome proliferation factors, such as clofibrate and 4-phenylbutyrate (Abe et al, 1998;Li et al, 2002). Our work has revealed light to be a significant peroxisome proliferator in plants, which strongly up-regulates the expression of the Arabidopsis PEX11b gene.…”

Modulation of the peroxisomal gene expression pattern by dehydroepiandrosterone and vitamin D: therapeutic implications

“…This is consistent with the strong induction of Pex 11 . Pex 11 and are involved in peroxisome proliferation (Abe et al 1998, Passreiter et al 1998, Schrader et al 1998. The enlarged size of part of the peroxisomes might possibly result from a disequilibrium between a very moderate increase in number of peroxisomes (1·6 times) and a strong induction of several peroxisomal enzymes.…”

“…A particularly strong increase (15-18 times) of Pex 11 was seen. Induction of its gene product in rat liver was described after treatment with thyroid hormone (Passreiter et al 1998) and clofibrate (Abe et al 1998). In yeast, disruption of Pex 11 caused oleate-induced giant peroxisomes (Erdmann & Blobel 1995) while overexpression resulted in peroxisome proliferation (Marshall et al 1995).…”

ArabidopsisPEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle–Associated Replication of Peroxisomes

“…The PEX11 family in mammals is composed of three members (PEX11a, PEX11b, and PEX11g) (Abe et al, 1998;Schrader et al, 1998b;Li et al, 2002aLi et al, , 2002bLi and Gould, 2002) and yeasts (Pex11p, Pex25p, and Pex27p) Smith et al, 2002;Rottensteiner et al, 2003;Tam et al, 2003). By contrast, the PEX11 family in Arabidopsis thaliana is composed of five members (PEX11a, PEX11b, PEX11c, PEX11d, and PEX11e) , which can be separated into three groups based on sequence homology: PEX11a, PEX11b, and PEX11c-e .…”

“…Overexpression of PEX11 homologs in mammals, plants, trypanosomes, and fungi leads to profound increases in peroxisome number, suggestive of a role for PEX11 proteins in the promotion of peroxisome fission Abe et al, 1998;Lorenz et al, 1998;Schrader et al, 1998b;Li et al, 2002aLi et al, , 2002bLi and Gould, 2002;Smith et al, 2002;Rottensteiner et al, 2003;Tam et al, 2003;Lingard and Trelease, 2006;Orth et al, 2007). Further evidence for the involvement of these proteins in peroxisome fission comes from cells lacking PEX11 isoforms.…”