Light Induces Peroxisome Proliferation in Arabidopsis Seedlings through the Photoreceptor Phytochrome A, the Transcription Factor HY5 HOMOLOG, and the Peroxisomal Protein PEROXIN11b

Desai, Mintu; Hu, Jianping

doi:10.1104/pp.107.113555

Cited by 81 publications

(90 citation statements)

References 78 publications

(117 reference statements)

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“…Although these isoforms appear to play different roles in peroxisomal proliferation, functional redundancy among various isoforms has been observed in Arabidopsis plants, as deletion of a single isoform does not significantly change the plant's physiology (Orth et al, 2007). Light-induced peroxisomal proliferation involving the peroxine PEX11b has been well characterized (Desai and Hu, 2008), while salinity-induced peroxisomal proliferation was regulated by PEX11e (Mitsuya et al, 2010). In addition to its role in proliferation, our results demonstrate that PEX11a controls peroxule formation, as the RNA i lines of PEX11a (pex11a) are unable to produce peroxules in response to Cd and As treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Proliferation of peroxisomes has been associated with stress conditions such as ozone (Oksanen et al, 2003), light (Desai and Hu, 2008), xenobiotics (de Felipe et al, 1988;Palma et al, 1991;Castillo et al, 2008), and salinity (Mitsuya et al, 2010). López-Huertas et al (2000) have suggested that peroxisomal proliferation is regulated by ROS produced under different stress conditions, probably to cope with oxidative damage induced by various stimuli.…”

Section: Discussionmentioning

confidence: 99%

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Peroxisomes Extend Peroxules in a Fast Response to Stress via a Reactive Oxygen Species-Mediated Induction of the Peroxin PEX11a

et al. 2016

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Peroxisomes are highly dynamic and metabolically active organelles that play an important role in cellular functions, including reactive oxygen species (ROS) metabolism. Peroxisomal dynamics, such as the proliferation, movement, and production of dynamic extensions called peroxules, have been associated with ROS in plant cells. However, the function and regulation of peroxules are largely unknown. Using confocal microscopy, we have shown that treatment of Arabidopsis leaves with the heavy metal cadmium produces time course-dependent changes in peroxisomal dynamics, starting with peroxule formation, followed by peroxisome proliferation, and finally returning to the normal morphology and number. These changes during Cd treatment were regulated by NADPH oxidase (C and F)-related ROS production. Peroxule formation is a general response to stimuli such as arsenic and is regulated by peroxin 11a (PEX11a), as Arabidopsis pex11a RNA i lines are unable to produce peroxules under stress conditions. The pex11a line showed higher levels of lipid peroxidation content and lower expression of genes involved in antioxidative defenses and signaling, suggesting that these extensions are involved in regulating ROS accumulation and ROSdependent gene expression in response to stress. Our results demonstrate that PEX11a and peroxule formation play a key role in regulating stress perception and fast cell responses to environmental cues.Peroxisomes are highly versatile organelles that adapt to changes in their cellular environment through morphological and metabolic adjustments (Hu et al., 2012;Sandalio et al., 2013). Plant peroxisomes perform essential functions such as photorespiration and fatty acid b-oxidation, ureide, and phytohormone (auxin and jasmonic acid) metabolisms, and also act as a source of signal molecules such as reactive oxygen and nitrogen species (ROS and RNS; Sandalio and RomeroPuertas, 2015). Peroxisomes contain a large battery of antioxidants to control ROS and RNS accumulation (Sandalio and Romero-Puertas, 2015). These organelles proliferate in response to environmental cues through a complex process involving elongation, constriction, and fission (Hu et al., 2012;Baker and Paudyal, 2014). Deciphering the signaling pathways governing the regulation of peroxisome proliferation under different environmental and metabolic conditions presents a major challenge in this field of research. Before peroxisomal division, organelle elongation occurs in a process regulated by peroxins 11 (PEX11), with the final division requiring dynamin-like or dynamin-related proteins and fission proteins (Hu et al., 2012, Schrader et al., 2012. There is evidence to suggest that ROS are involved in regulating peroxisome proliferation, as some peroxisomal biogenesis genes (PEX) are transcriptionally regulated by H 2 O 2 in both plant and animal cells (López-Huertas et al., 2000). The formation of peroxisomal extensions, known as peroxules, has also been observed to be regulated by exogenous applications of H 2 O 2 (Sinclair et al., 2009;Ba...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Peroxisomes Extend Peroxules in a Fast Response to Stress via a Reactive Oxygen Species-Mediated Induction of the Peroxin PEX11a

et al. 2016

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“…We crossed pex14-2 plants to transgenic lines stably expressing 35Spro:YFP-PTS1 (44,45). In contrast to wild-type leaf cells, in which YFP-labeled peroxisomes appeared as punctate structures, YFP-PTS1 was partially cytosolic in pex14-2 plants (Fig.…”

Section: Sp1 Represses Peroxisome Functionmentioning

confidence: 99%

E3 ubiquitin ligase SP1 regulates peroxisome biogenesis in Arabidopsis

Pan

Satkovich

2016

Proc. Natl. Acad. Sci. U.S.A.

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Peroxisomes are ubiquitous eukaryotic organelles that play pivotal roles in a suite of metabolic processes and often act coordinately with other organelles, such as chloroplasts and mitochondria. Peroxisomes import proteins to the peroxisome matrix by peroxins (PEX proteins), but how the function of the PEX proteins is regulated is poorly understood. In this study, we identified the Arabidopsis RING (really interesting new gene) type E3 ubiquitin ligase SP1 [suppressor of plastid protein import locus 1 (ppi1) 1] as a peroxisome membrane protein with a regulatory role in peroxisome protein import. SP1 interacts physically with the two components of the peroxisome protein docking complex PEX13-PEX14 and the (RING)-finger peroxin PEX2. Loss of SP1 function suppresses defects of the pex14-2 and pex13-1 mutants, and SP1 is involved in the degradation of PEX13 and possibly PEX14 and all three RING peroxins. An in vivo ubiquitination assay showed that SP1 has the ability to promote PEX13 ubiquitination. Our study has revealed that, in addition to its previously reported function in chloroplast biogenesis, SP1 plays a role in peroxisome biogenesis. The same E3 ubiquitin ligase promotes the destabilization of components of two distinct protein-import machineries, indicating that degradation of organelle biogenesis factors by the ubiquitin-proteasome system may constitute an important regulatory mechanism in coordinating the biogenesis of metabolically linked organelles in eukaryotes.peroxisome biogenesis | E3 ubiquitin ligase | protein import | peroxin | SP1

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“…In addition to our limited understanding of peroxisome biogenesis from the ER, the proteins implicated in plant peroxisome division are redundantly encoded in plants and often also participate in division of mitochondria or chloroplasts, making it challenging to isolate the roles of peroxisome division in plant physiology. Moreover, although the peroxins that directly mediate peroxisome biogenesis and division are identified, the transcriptional regulation of plant PEX genes is understudied, and only a few factors involved in PEX11 expression are identified (Desai and Hu, 2008;Desai et al, 2017).…”

Section: Future Perspectivesmentioning

confidence: 99%

“…2). Plant peroxisomes proliferate during cell division (Lingard et al, 2008) and in response to salinity (Mitsuya et al, 2010;Fahy et al, 2017), light (Desai and Hu, 2008), and cadmium treatments (Rodríguez-Serrano et al, 2016). Division involves the PMP PEX11, which has five isoforms (a to e) in Arabidopsis (Lingard and Trelease, 2006).…”

Section: Peroxisome Division and Proliferationmentioning

confidence: 99%