ClO^– Induced Dual-Excitation Fluorescent Probes Responding to Diverse Testing Modes with Ratio Methodology

Abstract

Single-excitation ratio fluorescent probes have enabled the output signal with high signal-to-noise ratio, but are still plagued with technique challenges, including signal distortion and limited application scenario. Herein, a dual-excitation near-infrared (NIR) fluorescent probe P1 of coumarin derivatives is constructed, showing high signal output ability in the visible region and high tissue penetration depth ability in the NIR region. As NIR probe P1 selectively recognizes ClO − , the emission signal in the visible region (480 nm) of P1 is enhanced during the recognition process. Meanwhile, the NIR emission (830 nm) of the conjugated system is weakened, finally realizing that ClO − triggered the dual-excitation (720/ 400 nm) ratio fluorescence signal detection and monitoring. The signal of detection in vitro has high responsiveness. Meanwhile, in the process of NIR monitoring in vivo, positive contrast imaging of fluorescence is constructed, which can accurately monitor ClO − changes over time. The current dual-excitation fluorescence-based data calibration and/or comparison method improves the application of the traditional single-excitation ratio fluorescence strategy and provide innovative detection tools for accurate measurement of fluorescence detection, with detection/monitoring modes suitable for different physiological environments.

Fast-Responsive HClO-Activated Near-Infrared Fluorescent Probe for In Vivo Diagnosis of Inflammatory Bowel Disease and Ex Vivo Optical Fecal Analysis

“…However, X-ray examination carries the risk of exposure to ionizing radiation, endoscopy is invasive, and histological examination has the potential to cause secondary trauma. , In addition, stool testing shows poor specificity and can hardly provide real-time feedback for diagnosis . Compared to traditional methods, fluorescence imaging has attracted great attention in IBD detection in recent years due to its advantages in high sensitivity, noninvasiveness, superior spatiotemporal resolution, and real-time visualization. − Therefore, the development of highly sensitive fluorescent probes holds great significance in the diagnosis and treatment of IBD.…”