Clioquinol is a promising preventive morphological switching compound in the treatment of Candida infections linked to the use of intrauterine devices

Pippi, Bruna; Machado, Gabriella da Rosa Monte; Bergamo, Vanessa Zafaneli; Alves, Ricardo José; Andrade, Saulo Fernandes de

doi:10.1099/jmm.0.000850

Cited by 13 publications

(18 citation statements)

References 42 publications

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“…The ability of antifungal agents to inhibit biofilm formation in 96-well polystyrene microtiter plates was evaluated by the technique of Bachmann et al and Pippi et al with modifications [22,23]. The wells of the microtiter plate were pretreated with antifungal solution in different concentration ranges, where they remained in contact for 48 h at a temperature of 4-8°C.…”

Section: Inhibition Of Biofilm Formation (Ibf)mentioning

confidence: 99%

“…Inhibition was determined as a percentage relative to the untreated control (100%) (according to the Eq. 1, where A is the absorbance value) [23]. The assay was performed in triplicate.…”

Section: Inhibition Of Biofilm Formation (Ibf)mentioning

confidence: 99%

“…The ability of antifungal agents to remove preformed biofilm in 96-well polystyrene microtiter plates was evaluated by the technique of Ramage et al and Pippi et al with some modifications [23,25]. The inoculum was prepared in 0.85% (w/v) sterile saline solution (Dynamics, Diadema, São Paulo, Brazil) according to "Fungal inoculum" section, and biofilm formation was performed in the same way as in "Inhibition of biofilm formation (IBF)" section.…”

Section: Inhibition Of Biofilm Formation (Ibf)mentioning

confidence: 99%

“…The ability of antifungal agents to kill preformed biofilm cells in 96-well polystyrene microtiter plates was evaluated according to Ramage et al,Shuford et al, and Pippi et al with modifications[23,25,26]. The performance of the test oc-…”

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confidence: 99%

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Rational selection of antifungal drugs to propose a new formulation strategy to control Candida biofilm formation on venous catheters

et al. 2020

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Introduction Infections associated with medical devices are often related to colonization by Candida spp. biofilm; in this way, numerous strategies have been developed and studied, mainly in order to prevent this type of fungal growth. Aim Considering the above, the main objective of the present study is to make a rational choice of the best antifungal therapy for the in vitro treatment of the biofilm on venous catheters, proposing an innovative formulation of a film-forming system to coat the surface in order to prevent the formation of biofilms. Methodology Anidulafungin, fluconazole, voriconazole, ketoconazole, amphotericin B, and the association of anidulafungin and amphotericin B were tested against biofilms of C. albicans, C. tropicalis, and C. parapsilosis strains in microtiter plates and in a polyurethane catheter. Besides, anidulafungin, amphotericin B, and the combination of both were incorporated in a filmforming system and were evaluated against biofilm. Results The superior activity of anidulafungin was demonstrated in relation to the other antifungal agents. Although amphotericin B showed good activity, high concentrations were required. The combination showed a synergistic action, in solution and in the formulation, showing excellent results, with activity above 90%. Conclusion Due to the superiority of anidulafungin and the synergistic activity of the combination, these alternatives were the most promising options for use in a formulation proposal as a new strategy to combat the Candida spp. biofilm. These formulations demonstrated high in vitro performance in the prevention of biofilms, indicating that they are candidates with great potential for in vivo tests.

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Section: Inhibition Of Biofilm Formation (Ibf)mentioning

confidence: 99%

“…Inhibition was determined as a percentage relative to the untreated control (100%) (according to the Eq. 1, where A is the absorbance value) [23]. The assay was performed in triplicate.…”

Section: Inhibition Of Biofilm Formation (Ibf)mentioning

confidence: 99%

Section: Inhibition Of Biofilm Formation (Ibf)mentioning

confidence: 99%

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confidence: 99%

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Rational selection of antifungal drugs to propose a new formulation strategy to control Candida biofilm formation on venous catheters

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“…Clioquinol (21, Fig. 5) is a dihalogenated 8-hydroxyquinoline and antiseptic drug effective against multidrug resistant Candida (Pippi et al 2018). It blocks hyphal development thus preventing biofilm formation.…”

Section: Antifungal Activitymentioning

confidence: 99%

Quinolines and Quinolones as Antibacterial, Antifungal, Anti-virulence, Antiviral and Anti-parasitic Agents

Šenerović

Opsenica

Morić

et al. 2019

Advances in Experimental Medicine and Biology

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Infective diseases have become health threat of a global proportion due to appearance and spread of microorganisms resistant to majority of therapeutics currently used for their treatment. Therefore, there is a constant need for development of new antimicrobial agents, as well as novel therapeutic strategies. Quinolines and quinolones, isolated from plants, animals, and microorganisms, have demonstrated numerous biological activities such as antimicrobial, insecticidal, antiinflammatory, antiplatelet, and antitumor. For more than two centuries quinoline/quinolone moiety has been used as a scaffold for drug development and even today it represents an inexhaustible inspiration for design and development of novel semi-synthetic or synthetic agents exhibiting broad spectrum of bioactivities. The structural diversity of synthetized compounds provides high and selective activity attained through different mechanisms of action, as well as low toxicity on human cells. This review describes quinoline and quinolone derivatives with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic activities with the focus on the last 10 years literature.

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Novel Therapies for Biofilm-Based Candida spp. Infections

Černáková

Light

Salehi

et al. 2019

Advances in Experimental Medicine and Biology

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Clioquinol is a promising preventive morphological switching compound in the treatment of Candida infections linked to the use of intrauterine devices

Cited by 13 publications

References 42 publications

Rational selection of antifungal drugs to propose a new formulation strategy to control Candida biofilm formation on venous catheters

Rational selection of antifungal drugs to propose a new formulation strategy to control Candida biofilm formation on venous catheters

Quinolines and Quinolones as Antibacterial, Antifungal, Anti-virulence, Antiviral and Anti-parasitic Agents

Novel Therapies for Biofilm-Based Candida spp. Infections

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