Clinicopathological and phenotypic features of chronic NK cell lymphocytosis identified among patients with asymptomatic lymphocytosis

Abstract

This study demonstrated that chronic NK cell lymphocytosis, similar to monoclonal B lymphocytosis or T-cell clones, may account for asymptomatic lymphocytosis. There were no identifiable causes of the NK cell expansion. The variable phenotype may represent the heterogeneity and pathological features of NK lymphocytosis.

Relevance of sample preparation for flow cytometry

“…[1][2][3][4][5][6] Flow cytometry, through the presence or absence of specific surface antigens, detects abnormal cells and helps in identifying their lineage and maturation stage, detects abnormal cells through identification of antigen expression that differs from the normal. [1][2][3][4][5][6] Flow cytometry, through the presence or absence of specific surface antigens, detects abnormal cells and helps in identifying their lineage and maturation stage, detects abnormal cells through identification of antigen expression that differs from the normal.…”

“…Over the past decades, multiparameter flow cytometry has become the method of choice for the differential diagnosis of several hematological diseases, for the definition of prognostic factors, and for the identification of rare cell populations in peripheral blood (PB) and bone marrow samples (BM). [1][2][3][4][5][6] Flow cytometry, through the presence or absence of specific surface antigens, detects abnormal cells and helps in identifying their lineage and maturation stage, detects abnormal cells through identification of antigen expression that differs from the normal. The wider application of flow cytometry in diagnostic and research fields increases the need to distinguish between real and artifact positive signals given that antigen-antibody interactions, characterized by weak bindings, may involve unexpected molecules.…”

The utility of peripheral blood smear review for identifying specimens for flow cytometric immunophenotyping

“…NK cell lymphocytosis of uncertain clinical significance has also been described, and may have restricted expression of KIR antigens, CD158a, CD158b, CD158e, and CD94/NKG2A, suggesting clonality. 6,17 Of interest, increased NK cells, cytotoxic T-cells and gammadelta T-cells have been described at diagnosis in chronic myeloid leukemia (CML) and multiple myeloma, and are often monoclonal or oligoclonal. In addition, lymphocytosis has been described in patients with CML receiving treatment with the second generation tyrosine kinase inhibitor Dasatinib.…”