Clinicopathologic study of retinoblastoma including MIB-1, p53, and CD99 immunohistochemistry

Schwimer, Christopher J.; Prayson, Richard A.

doi:10.1053/adpa.2001.25406

Cited by 17 publications

(8 citation statements)

References 25 publications

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“…Our findings show a marked cell proliferation as evidenced by a high MI and high expression of MIB-1. The high MIB-1 expression in our study (mean 55.26%, range 20-90%) is in keeping with other studies 19,20 that have indicated a high-proliferative capacity of retinoblastoma.…”

Section: Discussionsupporting

confidence: 93%

The Apoptotic Paradox in Retinoblastoma

Sitorus¹,

Gumay²,

Valk³

2009

Annals of the New York Academy of Sciences

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The purpose of this study was to investigate the clinicopathological features and the expression of proteins involved in cell proliferation and the different pathways of apoptosis in retinoblastoma. Nineteen retinoblastoma patients were included, and mitotic index (MI) and apoptotic index (AI) were assessed. The expression of MIB-1, p53, caspase-3, Bcl-2, and Fas protein was assessed by immunohistochemistry. Mann-Whitney U test and Fisher's exact test were used for statistical comparison. High MI (mean 16.84, range 0-66) and high MIB-1 expression (mean 57.89, range 0-90) were found. The MI was significantly related to MIB-1 expression (P= 0.01). The tumors showed a high apoptotic index (mean 40.26, range 1-110), and the AI was associated with the mitotic index (P= 0.02). The caspase-3 expression was positively related to the AI (P= 0.03), although a small number of tumors with no significant or very low caspase-3 staining showed a high number of apoptotic cells, suggestive of a caspase-3-independent apoptosis pathway. Bcl-2 expression was not significantly related to AI (P= 0.07). No striking relationship was found in expression patterns of p53, Bcl-2, caspase-3, and Fas. In conclusion, we found that (1) cell proliferation and apoptosis are linked in retinoblastoma; (2) activation of effector caspase-3 induces apoptosis in retinoblastoma, but Bcl-2 overexpression does not prevent apoptosis in many tumors; (3) there is a p53-independent pathway in approximately one-quarter of cases; (4) the findings suggesting a caspase-3-independent pathway might lead to apoptosis in retinoblastoma; and, finally, we found no consistent pattern of expression of apoptotic and antiapoptotic molecules, suggesting that in retinoblastoma there is no preference for any single pathway of apoptosis. Confirmation of the results in a large set of tumors would be useful.

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Section: Discussionsupporting

confidence: 93%

The Apoptotic Paradox in Retinoblastoma

Sitorus¹,

Gumay²,

Valk³

2009

Annals of the New York Academy of Sciences

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“…p53 gene expression has been observed in 50–60% of patients with retinoblastoma. [ 16 ] In the present study, the authors found necrosis (70.5%), calcification (64.7%), and retinal detachment (58.8%) as common HRFs. Additionally, the incidence of various morphological parameters such as anterior chamber seeding, ciliary body involvement, iris involvement, choroid involvement, scleral invasion, extra scleral invasion and optic nerve infiltration were found ranging between 11% and 58% in the study participants.…”

supporting

confidence: 55%

Innovative ophthalmology

Natarajan¹

2014

Indian J Ophthalmol

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“…For retinoblastoma it was shown that the proliferation rate, instead of the apoptotic rate (4)(5)(6)(7)(8)(9) , and the vascular pattern (8-9) correlated better with the prognosis. Antigen Ki-67 reacts with a DNA associated antigen in the nuclei at all phases of the cell cycle except the resting phase and it is considered a gold standard for cell proliferation in many tissues (8,(10)(11) with the advantage related to its availability and technical easiness. Recent reports showed a positive correlation between P and clinical stage (5)(6) .…”

Section: Discussionmentioning

confidence: 99%

“…The clinical indicators for inherited disease are early appearance age, bilateral disease and positive family history. Quantitative immunohistochemical analysis of cell proliferation, apoptosis and angiogenesis, have been reported recently in oncology (4)(5)(6)(7)(8)(9)(10) , and in other areas in ophthalmology (11) . For retinoblastoma it was shown that the proliferation index, instead of the apoptotic rate (4)(5)(6)(7)(8)(9) , and the vascular pattern (8)(9) correlated better with the prognosis.…”

Section: Introductionmentioning

confidence: 99%