Clinical Value of Inflammatory Cytokines in Patients with Aneurysmal Subarachnoid Hemorrhage

Luo, Cong; Yao, Jiaxin; Bi, Haoran; Li, Zhen; Li, Ju; Xue, Guosong; Li, Ké; Zhang, Shenyang; Zan, Kun; Meng, Weixiao; Zhang, Zunsheng; Chen, Hao

doi:10.2147/cia.s362854

Cited by 12 publications

(12 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because understanding severity and progression is important for designing future treatments for aSAH patients, these miRNAs levels were analyzed with respect to various groups classified according to disease severity. When admitted to the hospital, patients with WFNS grades I–III were classified as mild, and those with grades IV–V were classified as severe ( Luo et al, 2022 ). As illustrated in Figure 4A , comparison of the severe and mild aSAH patients revealed that the miRNA expression levels of hsa-miR-193b-3p and hsa-miR-486-3p were significantly elevated and that levels of hsa-miR-369-3p and hsa-miR-410-3p were significantly lowered ( p < 0.001).…”

Section: Resultsmentioning

confidence: 99%

Diagnosis potential of subarachnoid hemorrhage using miRNA signatures isolated from plasma-derived extracellular vesicles

et al. 2023

View full text Add to dashboard Cite

The diagnosis and clinical management of aneurysmal subarachnoid hemorrhage (aSAH) is currently limited by the lack of accessible molecular biomarkers that reflect the pathophysiology of disease. We used microRNAs (miRNAs) as diagnostics to characterize plasma extracellular vesicles in aSAH. It is unclear whether they can diagnose and manage aSAH. Next-generation sequencing (NGS) was used to detect the miRNA profile of plasma extracellular vesicles (exosomes) in three patients with SAH and three healthy controls (HCs). We identified four differentially expressed miRNAs and validated the results using quantitative real-time polymerase chain reaction (RT-qPCR) with 113 aSAH patients, 40 HCs, 20 SAH model mice, and 20 sham mice. Exosomal miRNA NGS revealed that six circulating exosomal miRNAs were differentially expressed in patients with aSAH versus HCs and that the levels of four miRNAs (miR-369-3p, miR-410-3p, miR-193b-3p, and miR-486-3p) were differentially significant. After multivariate logistic regression analysis, only miR-369-3p, miR-486-3p, and miR-193b-3p enabled prediction of neurological outcomes. In a mouse model of SAH, greater expression of miR-193b-3p and miR-486-3p remained statistically significant relative to controls, whereas expression levels of miR-369-3p and miR-410-3p were lower. miRNA gene target prediction showed six genes associated with all four of these differentially expressed miRNAs. The circulating exosomes miR-369-3p, miR-410-3p, miR-193b-3p, and miR-486-3p may influence intercellular communication and have potential clinical utility as prognostic biomarkers for aSAH patients.

show abstract

Section: Resultsmentioning

confidence: 99%

Diagnosis potential of subarachnoid hemorrhage using miRNA signatures isolated from plasma-derived extracellular vesicles

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Characteristics of the 18 enrolled studies which contained 1,469 cases are shown in Table 1. Of these, 6 articles compared the relationship between CRP and the prognosis of patients with SAH [8, 9, 19, 22–24, 26, 32, 33]; 8 articles compared the associations between IL-6 and the prognosis of patients with SAH [8, 10, 21, 23, 25, 27–30, 33]; 3 articles compared the correlation between IL-10 and the outcome of patients with SAH [8, 10, 31]; and 4 articles compared the relations between TNF-α and the prognosis of patients with SAH [8, 10, 21, 33].…”

Section: Resultsmentioning

confidence: 99%

“…Forest plot of studies comparing the peripheral TNF-α levels between SAH patients with good and poor outcomes. patients with SAH is still controversial [8][9][10]28]. To our knowledge, there is no meta-analysis to assess the relationship between peripheral inflammatory factors and the prognosis of SAH, and our research fills this gap.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Relationship between Peripheral Blood Inflammatory Factors and Prognosis of Subarachnoid Hemorrhage: A Meta-Analysis

et al. 2023

Eur Neurol

View full text Add to dashboard Cite

Background: Subarachnoid hemorrhage (SAH) is a severe cerebrovascular event with high mortality and disability rate. Neuroinflammation is involved in the brain injury after SAH, but the exact association between SAH progression and peripheral blood inflammatory factors is unknown. Therefore, to determine the relationship between inflammatory factors and the prognosis of SAH, we performed a meta-analysis. Method: A systematic literature review was conducted in PubMed, EMBASE, and Cochrane Library. Studies comparing the relationship between inflammatory factors (C-reactive protein (CRP), interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor (TNF-α) and prognosis of SAH were included in the study. A random-effects meta-analysis was conducted based on mRS, GOS, and the occurrence of CVS, DCI, and DINDs. Sensitivity analysis was performed using the leave-one-out method. The New-castle–Ottawa Scale (NOS) for case-control studies was used to assess the quality of included studies. And for continuous variables, we calculated the mean difference (MD) with a 95% confidence interval (CI). Results: 1469 patients from 18 case-control studies met the inclusion criteria. The results found that patients in the good outcome group had significantly lower CRP levels than those in the poor outcome group (SMD: -1.15, 95% CI: -1.64– -0.66, p < 0.00001, I2 = 87%), and peripheral IL-6 levels were significantly lower in SAH patients with the good functional outcome than those with the poor functional outcome (SMD: -0.99, 95% CI: -1.48– -0.51, p < 0.0001, I2 = 88%). As for IL-10 (SMD: -0.28, 95% CI: -0.97– 0.42, p =0.43, I2 = 88%) and TNF-α (SMD: -0.40, 95% CI: -0.98– 0.19, p =0.18, I2 = 79%), due to the small number of studies, heterogeneity, and uncontrollable factors, robust conclusions cannot be drawn. Conclusion: SAH patients with good prognoses have significantly lower peripheral CRP and IL-6 levels. In addition, due to the small number of studies, heterogeneity, and uncontrollable factors, robust conclusions cannot be drawn for IL-10 and TNF-α. More high-quality studies are needed in the future to provide more specific recommendations for the clinical practice of inflammatory factors.

show abstract

“…Some studies have reported that inflammatory markers can be used as a predictor of disease outcome. Luo et al 61 reported that IL-6, IL-10, IL-8, IL-2 and TNF-a, might predict the poor prognosis of aSAH. In terms of inflammatory indices, the neutrophil-lymphocyte ratio (NLR) has been found to be capable of predicting poor functional outcomes in aSAH patients, 62 and more recently, it has been reported that the NLR could even distinguish between aSAH and non-aneurysmal SAH.…”

Section: Discussionmentioning

confidence: 99%

HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage

Azúa-López

Pezzotti

González-Díaz

et al. 2023

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

Aneurysmal subarachnoid hemorrhage (aSAH) is a devastating disease with high morbidity and mortality rates. Within 24 hours after aSAH, monocytes are recruited and enter the subarachnoid space, where they mature into macrophages, increasing the inflammatory response and contributing, along with other factors, to delayed neurological dysfunction and poor outcomes. High-density lipoproteins (HDL) are lipid-protein complexes that exert anti-inflammatory effects but under pathological conditions undergo structural alterations that have been associated with loss of functionality. Plasma HDL were isolated from patients with aSAH and analyzed for their anti-inflammatory activity and protein composition. HDL isolated from patients lost the ability to prevent VCAM-1 expression in endothelial cells (HUVEC) and subsequent adhesion of THP-1 monocytes to the endothelium. Proteomic analysis showed that HDL particles from patients had an altered composition compared to those of healthy subjects. We confirmed by western blot that low levels of apolipoprotein A4 (APOA4) and high of serum amyloid A1 (SAA1) in HDL were associated with the lack of anti-inflammatory function observed in aSAH. Our results indicate that the study of HDL in the pathophysiology of aSAH is needed, and functional HDL supplementation could be considered a novel therapeutic approach to the treatment of the inflammatory response after aSAH.

show abstract

Clinical Value of Inflammatory Cytokines in Patients with Aneurysmal Subarachnoid Hemorrhage

Cited by 12 publications

References 43 publications

Diagnosis potential of subarachnoid hemorrhage using miRNA signatures isolated from plasma-derived extracellular vesicles

Diagnosis potential of subarachnoid hemorrhage using miRNA signatures isolated from plasma-derived extracellular vesicles

Relationship between Peripheral Blood Inflammatory Factors and Prognosis of Subarachnoid Hemorrhage: A Meta-Analysis

HDL anti-inflammatory function is impaired and associated with high SAA1 and low APOA4 levels in aneurysmal subarachnoid hemorrhage

Contact Info

Product

Resources

About