Clinical Utility of Flow Cytometry in the Study of Erythropoiesis and Nonclonal Red Cell Disorders

Marked changes in red cell membrane proteins in hereditary spherocytosis: a proteomics approach

“…Our data were consistent with the findings reported in many previous studies, indicating that HS is associated with an abnormal cell cycle, carbohydrate metabolism and molecular transport. 13,14 In Network III, the altered proteins in HS were associated with cell-cell signaling and interactions, including fibrinogen, dystonin, MHC class II and myosin, consistent with the findings reported in a previous study. 15 Note that most of the identified proteins reported in Table 2 had probabilistic MS scores >73 or MS/MS scores >40 with p o 0.05 (which were considered as significant hits).…”

Use of Flow Cytometry in the In Vitro and In Vivo Analysis of Tolerance/Anergy Induction by Immunocamouflage

“…[Chesney et al, 2011, Dieterlen et al, 2011, Hedley et al, 2011, Hernandez-Fuentes & Salama, 2006, Mittag & Tarnok, 2011, Panzer & Jilma, 2011, Tung et al, 2007, Venet et al, 2011 Indeed, flow cytometry has proved to be an exceptionally powerful tool in assessing the rejection or acceptance of allogeneic tissues. Flow cytometry is essential in characterizing both in vitro and in vivo immunological response including cell proliferation (CSFE staining and murine…”

In vivo acoustic and photoacoustic focusing of circulating cells

“…In particular, this research tool can provide insights into immune system dysfunction through real-time enumeration of individual WBC subpopulations (e.g., neutrophils, lymphocytes, monocytes), detection of abnormal RBC subsets (e.g., sickle cells, aggregates, or infected by malaria parasites), study of blood cell epigenetics, rheology and chemistry ( in vivo needleless blood test), or analysis of an unknown cell composition in lymph flow. Indeed, because of the difficulties of lymph sampling, basic knowledge of biological processes in lymphatics is lacking, including quantities of lymphatic macromolecules (e.g., cytokines, fatty acids) and cells (e.g., macrophages, B and T cells) and their interactions with each other and with endothelial cells 49 50 51 52 . In addition, identification of WBC aggregates by means of closely located PA peaks 8 could be important for studying neutrophil–platelet interactions involved in several thrombotic and inflammatory disorders and in leukocytosis 18 .…”

“…As a result, despite the impressive application of in vivo flow cytometry for detecting single CTCs against the background of many blood cells in the detection volume 7 8 9 10 11 12 13 17 , the great potential of this method for counting individual blood cells and/or abnormal cells at high concentration has not yet been reported. However, it is important for many applications, including studies of the immune system, inflammatory processes, cell–cell interactions, cell rolling, aggregation, leukocytosis, and thrombotic and infectious disorders at the single-cell level 47 48 49 50 51 52 53 . Here we demonstrate methods for cell manipulation with an emphasis on focusing cells directly in blood and lymph vessels in vivo by means of gradient acoustic forces ( Figs 1 , 2 , 3 , 4 , 5 , 6 , Supplementary Figs S1–15 ).…”