In vivo acoustic and photoacoustic focusing of circulating cells

Galanzha, Ekaterina I.; Viegas, Mark; Malinsky, Taras I.; Melerzanov, Alexander; Juratli, Mazen A.; Sarimollaoglu, Mustafa; Nedosekin, Dmitry A.; Zharov, Vladimir P.

doi:10.1038/srep21531

Cited by 46 publications

(45 citation statements)

References 54 publications

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“…Magnetic trapping was shown feasible for flow velocities up to 5 cm/s, so that it may represent a promising approach for microsurgical extraction or laser ablation of these cells. Cell trapping has also been achieved with gradient acoustic forces induced by ultrasound and optoacoustic waves [292] (Fig. 13a).…”

Section: Applications Enabled By Optoacoustic Visualization Of Multmentioning

confidence: 99%

“…(a) Cells in lymph flow of a mouse mesentery vessel before (left) and after (right) being trapped with gradient acoustic forces induced by optoacoustic waves generated by irradiation with a linear laser beam. Adapted with permission from [292]. © 2016 - Macmillan Publishers Ltd. (b) Optoacoustic set-up for measuring the flow velocity of cells via time correlation of the optoacoustic signals generated by two consecutive laser pulses.…”

Section: Figmentioning

confidence: 99%

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Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

et al. 2017

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Visualization of dynamic functional and molecular events in an unperturbed in vivo environment is essential for understanding the complex biology of living organisms and of disease state and progression. To this end, optoacoustic (photoacoustic) sensing and imaging have demonstrated the exclusive capacity to maintain excellent optical contrast and high resolution in deep-tissue observations, far beyond the penetration limits of modern microscopy. Yet, the time domain is paramount for the observation and study of complex biological interactions that may be invisible in single snapshots of living systems. This review focuses on the recent advances in optoacoustic imaging assisted by smart molecular labeling and dynamic contrast enhancement approaches that enable new types of multiscale dynamic observations not attainable with other bio-imaging modalities. A wealth of investigated new research topics and clinical applications is further discussed, including imaging of large-scale brain activity patterns, volumetric visualization of moving organs and contrast agent kinetics, molecular imaging using targeted and genetically expressed labels, as well as three-dimensional handheld diagnostics of human subjects.

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Section: Applications Enabled By Optoacoustic Visualization Of Multmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

et al. 2017

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“…53 Due to this unique SPR splitting, gold nanorods have been widely employed for CTC detection using techniques like photoacoustic imaging. 70 The SPR peak of gold nanoshells can be tuned in the range from visible to the near-infrared region by changing the ratio of the core size to its shell thickness. Generally, they have an SPR band of 850-900 nm.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

<p>Is small smarter? Nanomaterial-based detection and elimination of circulating tumor cells: current knowledge and perspectives</p>

Gribko

Künzel

Wünsch

et al. 2019

IJN

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Circulating tumor cells (CTCs) are disseminated cancer cells. The occurrence and circulation of CTCs seem key for metastasis, still the major cause of cancer-associated deaths. As such, CTCs are investigated as predictive biomarkers. However, due to their rarity and heterogeneous biology, CTCs’ practical use has not made it into the clinical routine. Clearly, methods for the effective isolation and reliable detection of CTCs are urgently needed. With the development of nanotechnology, various nanosystems for CTC isolation and enrichment and CTC-targeted cancer therapy have been designed. Here, we summarize the relationship between CTCs and tumor metastasis, and describe CTCs’ unique properties hampering their effective enrichment. We comment on nanotechnology-based systems for CTC isolation and recent achievements in microfluidics and lab-on-a-chip technologies. We discuss recent advances in CTC-targeted cancer therapy exploiting the unique properties of nanomaterials. We conclude by introducing developments in CTC-directed nanosystems and other advanced technologies currently in (pre)clinical research.

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“…This allows the user to identify and further analyse IVM ROIs ex vivo, as exemplified by the immunohistochemical characterisation of the microenvironments of migratory and non-migratory breast cancer cells (Ritsma et al, 2013a). Recent developments in intravital focusing of cells (Galanzha et al, 2016) or in matrix-assisted laser desorption/ionisation (MALDI) imaging (Grey et al, 2009) may open further avenues for combining IVM data with either in vivo flow cytometry applications or spatial proteomics.…”

Section: Future Applications and Combined Imaging Modesmentioning

confidence: 99%

Molecular mobility and activity in an intravital imaging setting – implications for cancer progression and targeting

Nobis

Warren

Lucas

et al. 2018

Journal of Cell Science

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Molecular mobility, localisation and spatiotemporal activity are at the core of cell biological processes and deregulation of these dynamic events can underpin disease development and progression. Recent advances in intravital imaging techniques in mice are providing new avenues to study real-time molecular behaviour in intact tissues within a live organism and to gain exciting insights into the intricate regulation of live cell biology at the microscale level. The monitoring of fluorescently labelled proteins and agents can be combined with autofluorescent properties of the microenvironment to provide a comprehensive snapshot of in vivo cell biology. In this Review, we summarise recent intravital microscopy approaches in mice, in processes ranging from normal development and homeostasis to disease progression and treatment in cancer, where we emphasise the utility of intravital imaging to observe dynamic and transient events in vivo. We also highlight the recent integration of advanced subcellular imaging techniques into the intravital imaging pipeline, which can provide in-depth biological information beyond the singlecell level. We conclude with an outlook of ongoing developments in intravital microscopy towards imaging in humans, as well as provide an overview of the challenges the intravital imaging community currently faces and outline potential ways for overcoming these hurdles.

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In vivo acoustic and photoacoustic focusing of circulating cells

Cited by 46 publications

References 54 publications

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

<p>Is small smarter? Nanomaterial-based detection and elimination of circulating tumor cells: current knowledge and perspectives</p>

Molecular mobility and activity in an intravital imaging setting – implications for cancer progression and targeting

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