Clinical Usefulness of Real-time PCR and Amplicor MTB PCR Assays for Diagnosis of Tuberculosis

Jung, Chan‐Young; Kim, Mi Kyung; Seo, Dong Chun; Lee, Mi Ae

doi:10.5145/kjcm.2008.11.1.29

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…One study in Korea showed the sensitivity and the specificity of the AdvanSure to be 97.9% and 100%, respectively, whereas 91% and 87%, respectively, in the other study, although there are only a fewer reports on the sensitivity and specificity of TB detection by real-time PCR 12,13. These two studies are different in their sample composition; the former used only respiratory samples that comprised sputum and bronchoalveolar lavage (BAL), while the latter included non-respiratory samples such as body fluid and urine, which made up 30.5% of the sample group 13,14. Therefore, it is highly possible that one of the causes for the different estimates of sensitivity and specificity is the difference in the sample composition.…”

Section: Discussionmentioning

confidence: 97%

“…12 , 13 These two studies are different in their sample composition; the former used only respiratory samples that comprised sputum and bronchoalveolar lavage (BAL), while the latter included non-respiratory samples such as body fluid and urine, which made up 30.5% of the sample group. 13 , 14 Therefore, it is highly possible that one of the causes for the different estimates of sensitivity and specificity is the difference in the sample composition. In particular, PCR inhibitory materials in non-respiratory samples can result in a lower sensitivity when performing real-time PCR on those samples.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Diagnostic Performance of Three Real-Time PCR Kits for DetectingMycobacteriumSpecies

et al. 2011

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PurposePCR is widely used for rapidly and accurately detecting Mycobacterium Species. The purpose of this study was to assess the diagnostic performance of three real-time PCR kits and evaluate the concordance with two older PCR methods.Materials and MethodsUsing 128 samples, the five PCR methods were assessed, including an in-house PCR protocol, the COBAS Amplicor MTB, the COBAS TaqMan MTB, the AdvanSure TB/NTM real-time PCR, and the Real-Q M. tuberculosis kit. The discrepant results were further examined by DNA sequencing and using the AdvanSure Mycobacteria Genotyping Chip for complete analysis.ResultsFor Mycobacterium tuberculosis (MTB) detection, all five kits showed 100% matching results (positive; N = 11 and negative; N = 80). In non-tuberculous mycobacterium (NTM) discrimination, the AdvanSure yielded two true-positive outcomes from M. intracellulare and one false positive outcome, while the Real-Q resulted in one true-positive outcome and one false negative outcome for each case and another false negative result using the provided DNA samples.ConclusionReal-time PCR, yielded results that were comparable to those of the older PCR methods for detecting MTB. However, there were disagreements among the applied kits in regard to the sample test results for detecting NTM. Therefore, we recommend that additional confirmatory measures such as DNA sequencing should be implemented in such cases, and further research with using a larger numbers of samples is warranted to improve the detection of NTM.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Comparison of Diagnostic Performance of Three Real-Time PCR Kits for DetectingMycobacteriumSpecies

et al. 2011

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“…However; the sensitivity rates have been varied considerably for SNss in the reported studies. [20][21][22][23][24][25][26][27][28] For most commercial tests, the assay sensitivities (87.5%-100%) seem to be satisfactory for AFB SPss, but the sensitivities (50.0% to 70.8%) varied greatly for AFB SNss. [11][12][13][14][15][16] Within these NAATs; Cobas Amplicor MTB and GenProbe MTD systems were commonly used for rapid diagnosis of MTBC in sputum samples.…”

Section: Discussionmentioning

confidence: 99%

“…From these systems, real-time PCR technology has replaced with the methodology of microbiological diagnosis using an automated system based on increased sensitivity. [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] The aim of this study was to evaluate the diagnostic performance of the nine commercial NAATs (COBAS Amplicor MTB-Method A, GenProbe MTD -Method B, Cobas TaqMan MTB PCR-Method C, iCycler iQ real-time PCR-Method D, TaqMan PCR AB 5700-Method E, TaqMan PCR AB 7700-Method F, LightCycler® 480Real-Time PCR System-Method G, Rotor Gene Real Time PCR -Method H and the AdvanSure TB/NTM Real-Time PCR -Method I.…”

mentioning

confidence: 99%

Diagnostic value of nine nucleic acid amplification test systems for Mycobacterium tuberculosis complex

Tarhan¹,

Cesur²,

Şimşek³

et al. 2015

Journal of Microbiology and Infectious Diseases

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Objective: In this study, nine commercial Nucleic Acid Amplification Test Systems (NAATs) were evaluated for diagnostic performance of Mycobacterium tuberculosis complex (MTBC) from smear positive sputum species (SPss) and smear negative sputum specimens (SNss). Methods: Sixty SPss and 55 SNss were examined microscopically by Ehrlich Ziehl Neelsen (EZN) staining method, and also inoculated on Löwenstein Jensen (LJ) medium for culture. The sensitivity and specificity of nine NAATs were calculated according to LJ culture method accepted as gold standard. Results: When LJ culture results were taken as gold standard; the sensitivity rates of method COBAS Amplicor MTB (Method A), GenProbe MTD (Method B), Cobas TaqMan MTB PCR (Method C), iCycler iQ RT PCR (Method D), TaqMan PCR AB 5700 (Method E), TaqMan PCR AB7700 (Method F), LightCycler® 480 RT PCR (Method G), Rotor Gene RT PCR (Method H) and the AdvanSure TB/NTM RT PCR (Method I) for SPss were 98.3 %, 93.3 %, 96.7 %, 100 %, 93.3 %, 100 %, 100 %, 100 % and 100 %, respectively. The sensitivity was 53.84% for the methods A, B, D, E, G and I; 38.46% for the method C and H; 61.5% for the method F for the method I in SNss. There were no statistical significant differences between the nine NAATs (p≥0.05). The specificity was 100% for all nine NAATs in SNss. The positivity rates of methods were 53.8% for methods A, B, D, E, G, I; 38.5% for methods C and H, and 61.5% for method F in SNss. These rates were 100% for D, F, G, H and I; 98.3% for method A; 96.7% for method C; 93,3% for methods B and E in SPss. Statistical analysis showed that there was no statistically significant differences among the nine NAATs (p≥0.05). Conclusion: It is concluded that the nine NAATs might be useful for detecting MTBC from SPss, but not effective for SNss.

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“…In other studies evaluating Advansure TB/NTM real-time PCR, PCR positivity rates for NTM were 55.6% and 67.0% each studies among samples culture-positive for NTM 6,10 . The lower concordant rate in our study may be due to the small number of NTM-positive samples, so it will be necessary to study the NTM detection capacity of TB/NTM real-time PCR with a larger study group.…”

Section: Discussionmentioning

confidence: 84%

Comparison of Ogawa Media, BACTEC MGIT 960 System and TB/NTM Real-Time PCR for DetectingMycobacteriumSpecies

2011

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Background: Mycobacterial infection is a problem throughout the world along with the increase of immunocompromised patients. For this reason, there have been many methods for faster and more accurate diagnosis. In this study, we evaluated several laboratory methods for mycobacterial infection. In the Mycobacteria genotyping chip analysis, the most frequently identified NTM species in descending order were M. avium, M. intracellulare, M. chelonae and M. abscessus. Conclusion: Culturing with a combination of MGIT and Ogawa is recommended to increase the recovery rate of mycobacteria. Although PCR missed a reasonable number of NTM, it is faster and usually gives results that concur with those from the culture. The appropriate combination of diagnostic methods with clinical correlation are necessary.

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Clinical Usefulness of Real-time PCR and Amplicor MTB PCR Assays for Diagnosis of Tuberculosis

Cited by 10 publications

References 24 publications

Comparison of Diagnostic Performance of Three Real-Time PCR Kits for DetectingMycobacteriumSpecies

Comparison of Diagnostic Performance of Three Real-Time PCR Kits for DetectingMycobacteriumSpecies

Diagnostic value of nine nucleic acid amplification test systems for Mycobacterium tuberculosis complex

Comparison of Ogawa Media, BACTEC MGIT 960 System and TB/NTM Real-Time PCR for DetectingMycobacteriumSpecies

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