Comparison of Ogawa Media, BACTEC MGIT 960 System and TB/NTM Real-Time PCR for Detecting<i>Mycobacterium</i>Species

Bang, Hae In; Choi, Tae Youn; Shin, Jeong Won

doi:10.4046/trd.2011.71.4.249

Cited by 4 publications

(2 citation statements)

References 10 publications

(13 reference statements)

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“…These conventional tests can take several weeks to perform and cannot always precisely identify the species6. Furthermore, these testing procedures are complex and laborious, and they are usually impeded by the slow growth of mycobacteria in culture.…”

Section: Introductionmentioning

confidence: 99%

Identification and Distribution of Nontuberculous Mycobacteria from 2005 to 2011 in Cheonan, Korea

Kim

Rheem

2013

Tuberc Respir Dis

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BackgroundNontuberculous mycobacteria (NTM) are considered opportunistic pathogens, and several species of NTM are associated with human diseases that typically involve the pulmonary, skin/soft tissue, or lymphatic systems; such infection may also cause disseminated diseases. Recent studies have reported increasing rates of NTM-induced disease worldwide.MethodsRespiratory samples are being analyzed for acid-fast bacilli (AFB) culture and NTM identification at Dankook University Hospital in Cheonan, Korea, from September 2005 to September 2011. Identification is performed by using polymerase chain reaction-restriction fragment length polymorphism analysis targeting a novel region of the rpoB gene.ResultsA total of 25,133 specimens were received for AFB culture, of which 1,014 (4.0%) were NTM-positive. A total of 267 samples from 186 patients were tested for NTM identifications, and 232 samples from 157 patients were positive for NTM species. Among the patients who tested positive for NTM, 65.6% were men and the average age was 63.3 years. Mycobacterium avium complex, the most commonly detected NTM pathogen, was found in 65.9% of the 232 samples. The annual average percentage of NTM isolates from AFB culture-positive specimens was 31.3%: the highest rate was seen in 2011 (44.3%), followed by 2009 (37.4%) and 2010 (37.2%). An upward trend in NTM incidence was found during the study period.ConclusionThe prevalence of pulmonary NTM isolates continues to increase in Cheonan, suggesting that pulmonary NTM disease is becoming increasingly common.

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Section: Introductionmentioning

confidence: 99%

Identification and Distribution of Nontuberculous Mycobacteria from 2005 to 2011 in Cheonan, Korea

Kim

Rheem

2013

Tuberc Respir Dis

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“…The AdvanSure TB/NTM real-time PCR kit (LG Life Science, Seoul, Korea) was developed to detect MTB and non-tuberculous mycobacteria (NTM); it targets the insertion sequence, IS6110 and internal transcribed spacer (ITS) region, respectively. Although this kit showed high sensitivity and specificity [ 3 - 5 ], its competency for quantification of bacterial burden has not been studied. In this study, we evaluated the quantitative performance of AdvanSure TB/NTM assay by comparing cycle threshold (Ct) values with 1) acid-fast bacilli (AFB) staining grades and semi-quantitative colony counts on solid medium and 2) time to positivity (TTP) in solid and liquid media.…”

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confidence: 99%

Assessment of the Quantitative Ability of AdvanSure TB/NTM Real-Time PCR in Respiratory Specimens by Comparison with Phenotypic Methods

et al. 2014

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Accurate quantification of mycobacterial load is important to evaluate disease severity and to monitor the course of treatment in tuberculosis (TB). We evaluated the quantitative capability of the AdvanSure TB/NTM real-time PCR kit (LG Life Science, Korea) to determine the cycle threshold (Ct) for mycobacterial burden. We retrospectively analyzed data from 108 patients whose respiratory specimens (sputums and bronchoalveolar lavage fluids) were positive for Mycobacterium tuberculosis complex (85 culture-positive and 23 culture-negative specimens). We compared Ct values with grades of acid-fast bacilli (AFB) staining, semi-quantitative colony count on solid medium, and time to positivity (TTP) in liquid and solid media. We also investigated the cutoff Ct value for predicting stain-positive status. Ct value showed significant reverse correlation with AFB staining grade (rs=-0.635, P<0.01). Ct value significantly decreased as the semi-quantitative counts on the solid medium increased (P<0.001), and the mean Ct value of each of the groups 1+, 2+, 3+, and 4+ were 29.0, 30.0, 27.1, and 25.5, respectively. A weak correlation between Ct value and TTP in liquid and solid media was observed (rs=0.468 and 0.365, respectively). A cutoff Ct value of <33.2 best predicted stain positivity, with a sensitivity of 95.0% and a specificity of 32.0%. Our findings suggest the potential use of AdvanSure TB/NTM real-time PCR kit for quantitatively determining bacterial burden, albeit with some enhancements.

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Usefulness of the BACTEC MGIT 960 System for Mycobacterial Culture and TB Ag MPT64 Immunochromatographic Assay to IdentifyMycobacterium tuberculosis

Lee

et al. 2012

Yeungnam Univ J Med

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Comparison of Ogawa Media, BACTEC MGIT 960 System and TB/NTM Real-Time PCR for DetectingMycobacteriumSpecies

Cited by 4 publications

References 10 publications

Identification and Distribution of Nontuberculous Mycobacteria from 2005 to 2011 in Cheonan, Korea

Identification and Distribution of Nontuberculous Mycobacteria from 2005 to 2011 in Cheonan, Korea

Assessment of the Quantitative Ability of AdvanSure TB/NTM Real-Time PCR in Respiratory Specimens by Comparison with Phenotypic Methods

Usefulness of the BACTEC MGIT 960 System for Mycobacterial Culture and TB Ag MPT64 Immunochromatographic Assay to IdentifyMycobacterium tuberculosis

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