Clinical significance of miR-19b-3p in patients with sepsis and its regulatory role in the LPS-induced inflammatory response

Abstract

Background: MicroRNAs (miRNAs) play important roles in the development and progression of sepsis. This study investigated the clinical value of miR-19b-3p in sepsis patients, and explored its role in regulating inflammatory responses in HUVECs cells. Methods: 103 patients with sepsis and 98 healthy individuals were recruited. qRT-PCR was used for the measurement of miR-19b-3p level. Cell viability was evaluated using CCK-8. The protein levels of TNF-α and IL-6 were measured using ELISA. Receiver operating characteristic (ROC) curve and logistic regression analysis were constructed to evaluate the diagnostic and prognostic values of miR-19b-3p in sepsis patients. Results: MiR-19b-3p level was significantly reduced in the serum from patients with sepsis compared with healthy controls (P < 0.001). Sepsis patients in the survival group had significantly high miR-19b-3p levels compared with the non-survival group (P < 0.001). MiR-19b-3p was of a good value in predicting sepsis risk, and was an independent prognostic factor for 28-day survival in sepsis patients (OR = 3.226, 95% CI 1.076-9.670, P = 0.037). MiR-19b-3p level was negatively associated with serum levels of IL-6 (r = − 0.852, P < 0.001) and TNF-α (r = − 0.761, P < 0.001). Overexpression of miR-19b-3p alleviated LPS-induced inflammatory response of HUVECs, which was reflected by the decrease of the levels of IL-6 and TNF-α induced by LPS treatment (P < 0.001). Conclusion: MiR-19b-3p might be a potential biomarker for the early diagnosis and prognosis of sepsis patients. Overexpression of miR-19b-3p alleviated sepsis-induced inflammatory responses.

Phosphorylated STAT3 suppresses microRNA‐19b/1281 to aggravate lung injury in mice with type 2 diabetes mellitus‐associated pulmonary tuberculosis

“…34 In addition, protective roles of miR-19b-3p in patients with sepsis have been identified as well by relieving the levels of pro-inflammatory factors IL-6 and TNF-α. 35 MiR-19b-3p was also reported to attenuate IL-1β-induced inflammatory injury in chondrocytes through targeting GRK6. 16 As for miR-1281, it has been identified as a tumour suppressor in many malignancies such as glioma, 17 osteosarcoma 36 and gastric cancer.…”

Accuracy of circulating microRNAs in diagnosis of sepsis: a systematic review and meta-analysis

“…After removing 1978 duplicates, animal or in vitro studies, irrelevant articles, and excluded article format, a total of 83 full-text references were screened for eligibility. Finally, 22 records were included in our systematic review [ 6 – 9 , 14 – 31 ]. The detailed flow diagram of the study selection process is shown in Fig.…”

“…Risk of bias is mainly derived from the index test. The conduct or interpretation of the index test introduced high risk in 6 records [ 7 , 8 , 17 , 19 , 23 , 28 ] and unclear risk in 13 articles [ 6 , 14 – 16 , 18 , 20 , 21 , 25 – 27 , 29 – 31 ].…”

“…The levels of PCT could help to guide the antibiotic therapy in patients with sepsis, while the heterogeneous data of PCT failed to provide guidance for the benefits of sepsis patients [ 34 , 35 ]. Current literature suggested miRNAs played a vital role in the pathophysiology of sepsis and could distinguish the various phases of sepsis [ 6 – 9 , 11 , 14 – 31 ]. Several miRNAs, such as miR-146-a, miR-125b, and miR-223, were positively or negatively associated with sepsis severity [ 22 , 25 , 28 ].…”

<p>Human Adipose-Derived Mesenchymal Stem Cells-Derived Exosomal microRNA-19b Promotes the Healing of Skin Wounds Through Modulation of the CCL1/TGF-β Signaling Axis</p>

“…A recent study has presented that miR-19b-3p level is markedly reduced in the serum from sepsis patients in relation to healthy controls. 33 Another study has presented that Figure 7 Exosome promotes wound healing in mice. (A) Wound healing state of mice treated with PBS, Exo-NC or Exo-inhibitor, respectively (two-way ANOVA, *P < 0.05 vs the PBS group; #P < 0.05 vs the Exo-NC group).…”