Rice (Oryza sativa), a major staple throughout the world and a model system for plant genomics and breeding, was the first crop genome sequenced almost two decades ago. However, reference genomes for all higher organisms to date contain gaps and missing sequences. Here, we report the assembly and analysis of gap-free reference genome sequences for two elite O. sativa xian/indica rice varieties, Zhenshan 97 and Minghui 63, which are being used as a model system for studying heterosis and yield. Gapfree reference genomes provide the opportunity for a global view of the structure and function of centromeres. We show that all rice centromeric regions share conserved centromere-specific satellite motifs with different copy numbers and structures. In addition, the similarity of CentO repeats in the same chromosome is higher than across chromosomes, supporting a model of local expansion and homogenization. Both genomes have over 395 non-TE genes located in centromere regions, of which $41% are actively transcribed. Two large structural variants at the end of chromosome 11 affect the copy number of resistance genes between the two genomes. The availability of the two gap-free genomes lays a solid foundation for further understanding genome structure and function in plants and breeding climate-resilient varieties.
Developing powerful and reliable strategies to covalently functionalize graphene for efficient grafting and achieving precise interface control remains challenging due to the strong interlayer cohesive energy and the surface inertia of graphene. Here, we present versatile and efficient grafting strategies to functionalize graphene nanosheets. An alkyne-bearing graphene core was used to prepare polymer-functionalized graphene using 'grafting to' and 'grafting from' strategies in combination with reversible chain transfer and click chemistry. The use of the 'grafting to' approach allows full control over limited length grafted polymer chains, while permitting a high grafting density to a single graphene face, resulting in good solubility and processability. The 'grafting from' approach offers complementary advantages, such as the grafting of high molecular weight polymer chains and a better coverage ratio on the graphene surface; however, the extra steps introduced, the presence of initiating groups, and difficulty in controlling the grafted polymer lead to decreased processability. Various types of polymer chains have been successful covalently tethered to graphene nanosheets using these two approaches, producing various molecular brushes with multifunctional arms resulting in watersoluble, oil-soluble, acidic, basic, polar, apolar, and variously functionalized polymers. This work describes versatile methodologies, using the 'grafting to' and 'grafting from' approaches, for the preparation of individually dispersed graphene nanosheets having the desirable properties described.
Purpose. In this study, we examined whether the associations between working hours, job satisfaction, and work-life balance are mediated by occupational stress. In addition, we tested whether perceived time control helps moderate the effects of working hours and occupational stress. Methodology. Questionnaires were administered to 369 respondents working in the high-tech and banking industries. Analyses were then conducted on the data. Findings. The analysis revealed significant correlations between long working hours and both occupational stress and work-life balance, as well as between occupational stress and both work-life balance and job satisfaction. In addition, the relationship between working hours and occupational stress exhibited a significantly positive interaction with perceived time control. Value. The results indicate the importance of giving workers greater control over working hours. We therefore recommend that labor laws should be revised as necessary to prevent excessive working hours and enhance work-time flexibility.
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