Clinical significance of microRNA-200 and let-7 families expression assessment in patients with ovarian cancer

Ferneza, Severyn; Fetsych, Markiyan; Shuliak, Roman; Makukh, Halyna; Volodko, Natalia; Yarema, Roman; Fetsych, Тaras

doi:10.3332/ecancer.2021.1249

Cited by 6 publications

(3 citation statements)

References 63 publications

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“…Abnormally expressed miRNAs in tumors play an important role in regulating metastasis, proliferation, multidrug resistance and even the self-renewal and differentiation of cancer stem cells [28]. MiRNAs have been used as markers to predict ovarian cancer sensitivity to platinum and taxane chemotherapeutic agents, which significantly improve treatment outcomes [29]. Some microRNAs are not conducive to cancer outcomes, such as microRNA-4491, which enhances proliferation and inhibits apoptosis in nonsmall cell lung cancer cells [30].…”

Section: Discussionmentioning

confidence: 99%

MiR-302c-5p affects the stemness and cisplatin resistance of nasopharyngeal carcinoma cells by regulating HSP90AA1

et al. 2022

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Nasopharyngeal carcinoma (NPC) is one of the most frequent malignant tumors diagnosed in China. Cisplatin is one of the most commonly used anticancer drugs containing platinum in combined chemotherapy. The molecular mechanism of NPC is still largely unknown, and we aim to spare no effort to elucidate it. Normal human nasopharyngeal epithelial cells and NPC cell lines were cultured. The expression levels of miR-302c-5p and HSP90AA1 were detected with quantitative real-time PCR. Western blotting was used to analyze levels of the HSP90AA1, protein kinase B (AKT), p-AKT, CD44 and SOX2 proteins. The interaction between miR-302c-5p and HSP90AA1 was detected using a luciferase reporter assay. The bicinchoninic acid assay was used to observe cisplatin resistance in NPC cells. Our records confirmed that the expression of miR-302c-5p was substantially reduced and HSP90AA1 was increased in NPC cells. Additionally, miR-302c-5p inhibited cisplatin resistance and the traits of stem cells in NPC. A luciferase assay confirmed that miR-302c-5p is bound to HSP90AA1. Overexpression of HSP90AA1 may reverse the effects of overexpressed miR-302c-5p and inhibit cisplatin resistance and stem cell traits of NPC. This study investigated whether miR-302c-5p inhibited the AKT pathway by regulating HSP90AA1 expression and altered the resistance of NPC cells to cisplatin and the traits of tumor stem cells, which has not yet been reported.

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Section: Discussionmentioning

confidence: 99%

MiR-302c-5p affects the stemness and cisplatin resistance of nasopharyngeal carcinoma cells by regulating HSP90AA1

et al. 2022

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“…With mRNA > miRNA, partial repression is only possible. In B, the effect of multiple mRNA per miRNA is indicated competing for the same miRNA; C the coordination of families is indicated compensating for the competition of multiple mRNA and mRNA diseases, development and cancer (Li and Mao 2007;Howe et al 2012;Servin-Gonzalez et al 2015;Granados-Lopez et al 2017;Jiang et al 2017;Balzano et al 2018;Yin et al 2018;Pinchi et al 2019;Ferneza et al 2021;Gregorova et al 2021). The family members can vary from 2 to 30 members (e.g.…”

Section: Evidence For An Emerging Role Of Families In Mrna Repressionmentioning

confidence: 99%

Mechanosensitive pathways are regulated by mechanosensitive miRNA clusters in endothelial cells

et al. 2021

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Shear stress is known to affect many processes in (patho-) physiology through a complex, multi-molecular mechanism, termed mechanotransduction. The sheer complexity of the process has raised questions how mechanotransduction is regulated. Here, we comprehensively evaluate the literature about the role of small non-coding miRNA in the regulation of mechanotransduction. Regulation of mRNA by miRNA is rather complex, depending not only on the concentration of mRNA to miRNA, but also on the amount of mRNA competing for a single mRNA. The only mechanism to counteract the latter factor is through overarching structures of miRNA. Indeed, two overarching structures are present miRNA families and miRNA clusters, and both will be discussed in details, regarding the latest literature and a previous conducted study focussed on mechanotransduction. Both the literature and our own data support a new hypothesis that miRNA-clusters predominantly regulate mechanotransduction, affecting 65% of signalling pathways. In conclusion, a new and important mode of regulation of mechanotransduction is proposed, based on miRNA clusters. This finding implicates new avenues for treatment of mechanotransduction and atherosclerosis.

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“…A Let-7 tem demonstrado atuar como reguladores diretos de alguns importantes oncogenes como HMGA2, STAT3, UHRF2, MYC, RAS e IGF. A desregulação de miRNAs Let-7 tem sido também relacionada em meduloblastoma e câncer de pulmão (LARREA et al, 2016;FERNEZA et al, 2021;FIALA et al, 2020).…”

Section: Introductionunclassified

DESREGULAÇÃO DE microRNAs EM PROCESSOS PATOLÓGICOS DO CÂNCER

Padilha¹,

Feitosa²

2022

RECIMA21

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miRNAs são moléculas endógenas de 19 a 25 nucleotídeos, essas moléculas são transcritas pela RNA polimerase II, após a transcrição, os microRNAs são processados por uma RNAse III chamada DROSHA e uma proteína de ligação ao RNA dupla fita DGCR8; a DICER se associa com a proteína TRBP (proteína de ligação a RNA responsiva a ativação gênica) na clivagem do duplex de miRNA, com as proteínas do complexo de silenciamento induzido por RISC ocasionando regulação negativa pós-transcricional do mRNA alvo. Os miRNAs estão envolvidos em vários processos biológicos, como regulação da homeostase, diferenciação da linhagem hematopoiética, sendo relacionados também a processos patológicos como câncer e várias outras doenças. Esse estudo é uma revisão integrativa, as bases de dados consultadas foram o NCBI, BVS, SciELO e lista de referências dos artigos selecionados, foram baixados 156 artigos e apenas 36 foram incluídos na revisão, sendo artigos em português e inglês; utilizamos o fluxograma PRISMA 2020 para triagem dos artigos. Os achados demonstraram ampla gama de interações movidas por amplo espectro de proteínas, a regulação positiva e negativa dos miRNAs tem sido associada vários tipos de câncer, essas moléculas podem atuar como oncogenes ou genes supressores de tumor e tem se tornado um alvo atraente para estudos terapêuticos, o que poderia guiar mecanismos mais eficazes baseados na tomada de decisão clínica como prognóstico, diagnóstico e até como procedimento não invasivo.

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Clinical significance of microRNA-200 and let-7 families expression assessment in patients with ovarian cancer

Cited by 6 publications

References 63 publications

MiR-302c-5p affects the stemness and cisplatin resistance of nasopharyngeal carcinoma cells by regulating HSP90AA1

MiR-302c-5p affects the stemness and cisplatin resistance of nasopharyngeal carcinoma cells by regulating HSP90AA1

Mechanosensitive pathways are regulated by mechanosensitive miRNA clusters in endothelial cells

DESREGULAÇÃO DE microRNAs EM PROCESSOS PATOLÓGICOS DO CÂNCER

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