Mechanosensitive pathways are regulated by mechanosensitive miRNA clusters in endothelial cells

Herault, Sean; Naser, J; Carassiti, Daniele; Chooi, Kok Yean; Nikolopoulou, Rosa; Font, Marti Llopart; Patel, M.; Pedrigi, Ryan M.; Krams, Rob

doi:10.1007/s12551-021-00839-0

Cited by 4 publications

(2 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LIS1 OE in the absence of AGO1-4 resulted in a slight but significant increase in a small set of miRs. Data mining revealed that many of these miRs, including members of the let-7 family, miR-221-3p, 16-5p, 296-3p, and others, are involved in mechanotransduction pathways 60, 77–85 . RNA seq data further revealed the DE of genes associated with mechanosensitive pathways.…”

Section: Discussionmentioning

confidence: 99%

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways

Kshirsagar

Gorelik

Olender

et al. 2022

Preprint

View full text Add to dashboard Cite

Lissencephaly-1 (LIS1) is associated with neurodevelopmental diseases and is known to regulate the activity of the molecular motor cytoplasmic dynein. Here we show that LIS1 is essential for the viability of mouse embryonic stem cells (mESCs), and it regulates the physical properties of these cells. LIS1 dosage substantially affects gene expression, and we uncovered an unexpected interaction of LIS1 with RNA and RNA-binding proteins, most prominently the Argonaute complex. We demonstrate that LIS1 overexpression partially rescued the expression of extracellular matrix (ECM) and mechanosensitive genes conferring stiffness to Argonaute null mESCs. Collectively, our data transforms the current perspective on the roles of LIS1 in post-transcriptional regulation underlying development and mechanosensitive processes.

show abstract

Section: Discussionmentioning

confidence: 99%

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways

Kshirsagar

Gorelik

Olender

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…LIS1 OE in the absence of AGO1-4 resulted in a slight but significant increase in a small set of miRs. Data mining revealed that many of these miRs, including members of the let-7 family, miR-221-3p, 16-5p, 296-3p, and others, are involved in mechanotransduction pathways 67 , 88 – 96 . RNA seq data further revealed the DE of genes associated with mechanosensitive pathways.…”

Section: Discussionmentioning

confidence: 99%

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways

Kshirsagar,

Doroshev,

Gorelik

et al. 2023

Nat Commun

View full text Add to dashboard Cite

Lissencephaly-1 (LIS1) is associated with neurodevelopmental diseases and is known to regulate the molecular motor cytoplasmic dynein activity. Here we show that LIS1 is essential for the viability of mouse embryonic stem cells (mESCs), and it governs the physical properties of these cells. LIS1 dosage substantially affects gene expression, and we uncovered an unexpected interaction of LIS1 with RNA and RNA-binding proteins, most prominently the Argonaute complex. We demonstrate that LIS1 overexpression partially rescued the extracellular matrix (ECM) expression and mechanosensitive genes conferring stiffness to Argonaute null mESCs. Collectively, our data transforms the current perspective on the roles of LIS1 in post-transcriptional regulation underlying development and mechanosensitive processes.

show abstract

Cardiovascular mechanobiology—a Special Issue to look at the state of the art and the newest insights into the role of mechanical forces in cardiovascular development, physiology and disease

Swiatlowska

Iskratsch

2021

Biophys Rev

View full text Add to dashboard Cite

There has been much progress recently in the area of cardiovascular mechanobiology and this Special Issue aims at taking stock. This editorial gives context of the main motivation for this special issue as well as a brief summary of its content.Cardiovascular diseases (CVD) are the primary cause of mortality, and the disease burden is projected to grow because of sedentary lifestyles and an ageing society. Vascular diseases and especially atherosclerosis, if untreated, are the dominant underlying cause of CVD. Moreover, cardiac disease progressing to heart failure is the leading cause of death among cardiovascular diseases. Underlying genetic causes and mechanisms are progressively being uncovered, e.g. through Genome-Wide Association Studies (GWAS)

show abstract

Mechanosensitive pathways are regulated by mechanosensitive miRNA clusters in endothelial cells

Cited by 4 publications

References 83 publications

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways

Cardiovascular mechanobiology—a Special Issue to look at the state of the art and the newest insights into the role of mechanical forces in cardiovascular development, physiology and disease

Contact Info

Product

Resources

About