Clinical significance of matrix metalloproteinase-2 in cancer of uterine cervix: A semiquantitative study of immunoreactivities using tissue array

Wang, Po-Hui; Ko, Jiunn‐Liang; Tsai, Hsiu Ting; Yang, Shun‐Fa; Han, Chih-Ping; Lin, Long-Yau; Chen, Gin‐Den

doi:10.1016/j.ygyno.2007.11.018

Cited by 21 publications

(28 citation statements)

References 34 publications

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“…MMP-2 is correlated with poor survival in prostate cancer (38), HCC (39), and breast cancer (40). In addition, upregulation of MMP-2 protein expression has been observed in cervical cancer tissues and is associated with LNM (23). In this study, we found that MMP-2 is an independent prognostic factor for LNM in HCC and may be a useful predictive marker for LNM in HCC.…”

Section: Discussionsupporting

confidence: 53%

“…Heatmaps are shown in Figure 1. On the basis of previous reports (17,(20)(21)(22)(23), we chose HIF-1a, VEGF, and MMP-2 for further analysis. Immunohistochemistry and TMA analyses of these proteins were conducted with an independent cohort of 309 HCC patients to evaluate the ability of these potential biomarkers to predict LNM.…”

Section: Rna Isolation and Qrt-pcrmentioning

confidence: 99%

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Gene Expression Profiling of Fixed Tissues Identified Hypoxia-Inducible Factor-1α, VEGF, and Matrix Metalloproteinase-2 as Biomarkers of Lymph Node Metastasis in Hepatocellular Carcinoma

Xiang

Ren

Fan

et al. 2011

Clinical Cancer Research

112

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Purpose: Hepatocellular carcinoma (HCC) most often develops in patients infected with hepatitis B or hepatitis C virus. Differential gene expression profiling is useful for investigating genes associated with lymph node metastasis (LNM). We screened genes to identify potential biomarkers for LNM in HCC.Experimental Design: RNA was extracted from formalin-fixed specimens of paired intratumoral and peritumoral tissues of patients with lymph node-positive (n ¼ 36) or negative (n ¼ 36) HCC. A cDNAmediated annealing, selection, extension, and ligation assay was done with an array of 502 known cancerrelated genes to identify differentially expressed genes in 20 pairs of patients with or without LNM. Candidate biomarkers were evaluated by using immunohistochemistry and tissue microarrays in an independent cohort of 309 HCC patients who had undergone hepatectomy. Of the 309 patients, 235 (76.1%) patients were infected with hepatitis B.Results: Compared with lymph node-negative patients, lymph node-positive patients had 17 overexpressed genes and 19 underexpressed genes in intratumoral tissues, and 25 overexpressed genes and 22 underexpressed genes in peritumoral tissues. Hypoxia-inducible factor (HIF)-1a, VEGF, and matrix metalloproteinase (MMP)-2 were selected for analysis in the cohort of 309 HCC patients. We found that intratumoral protein levels of HIF-1a, VEGF, and MMP-2 were independent risk factors for developing LNM.Conclusion: We identified 83 cancer genes that were differentially expressed in lymph node-positive and lymph node-negative HCC. Our findings show that the combination of intratumoral HIF-1a, VEGF, and MMP-2 may be useful as a molecular prediction model for LNM.

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Section: Discussionsupporting

confidence: 53%

Section: Rna Isolation and Qrt-pcrmentioning

confidence: 99%

Gene Expression Profiling of Fixed Tissues Identified Hypoxia-Inducible Factor-1α, VEGF, and Matrix Metalloproteinase-2 as Biomarkers of Lymph Node Metastasis in Hepatocellular Carcinoma

Xiang

Ren

Fan

et al. 2011

Clinical Cancer Research

112

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“…During the last decades progress in research on enzyme activities showed the potential significance of MMP-2 and MMP-9 in the progression of cervical uterine cancer suggesting their prognostic value (14,15,(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64)(65). Sheu et al demonstrated that MMP-2 and MMP-9 were overexpressed in >90% of squamous cell carcinomas (SCC) and 83-100% of HSIL, but were less frequently expressed in LSIL and normal squamous epithelium (13%).…”

Section: Mmps In Uterine Cervical Neoplasmmentioning

confidence: 99%

Uterine cervical carcinoma: Role of matrix metalloproteinases (Review)

Libra¹

2009

Int J Oncol

116

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Abstract.Epidemiological and experimental studies have provided evidence that human papillomavirus (HPV) infection is a main player in the development of uterine cervical neoplasms. Migration of cancer cells from the origin tissue to surrounding or distant organs is essential for tumor progression. Many studies of tumor invasion and metastases have focused on the degradation of the extracellular matrix where matrix metalloproteinases (MMPs) play a central role. Two of these enzymes, MMP-2 and MMP-9, have been correlated with the processes of tumor cell invasion and metastasis in human cancers, including uterine neoplasms. It has been shown that the up-regulation of MMPs is associated with progression of cervical uterine neoplasms. This review describes the current understanding of MMP-2 and MMP-9 expression and activity in pre-cancer and cancer lesions of cervical uterine, which may open new strategies for diagnostic and therapeutic interventions.

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“…In patients with low-grade squamous intraepithelial lesions of the uterine cervix, increased viral load results in high risk of developing high-grade squamous intraepithelial lesions comparing to low viral load. 27 On the contrary, in cervical carcinoma, low or negative viral load was associated with poor cancer prognosis. [28][29][30] siRNA induced increased expression of MMP-2 protein, which was known as having a crucial role of tumor invasion and metastasis in cervical carcinoma.…”

Section: Resultsmentioning

confidence: 99%

Low dynamin 2 expression is associated with tumor invasion and metastasis in invasive squamous cell carcinoma of cervix

Lee¹,

Do²,

Park³

et al. 2010

Cancer Biology & Therapy

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Clinical significance of matrix metalloproteinase-2 in cancer of uterine cervix: A semiquantitative study of immunoreactivities using tissue array

Cited by 21 publications

References 34 publications

Gene Expression Profiling of Fixed Tissues Identified Hypoxia-Inducible Factor-1α, VEGF, and Matrix Metalloproteinase-2 as Biomarkers of Lymph Node Metastasis in Hepatocellular Carcinoma

Gene Expression Profiling of Fixed Tissues Identified Hypoxia-Inducible Factor-1α, VEGF, and Matrix Metalloproteinase-2 as Biomarkers of Lymph Node Metastasis in Hepatocellular Carcinoma

Uterine cervical carcinoma: Role of matrix metalloproteinases (Review)

Low dynamin 2 expression is associated with tumor invasion and metastasis in invasive squamous cell carcinoma of cervix

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