Clinical significance of expression of proliferating cell nuclear antigen and E-cadherin in gastric carcinoma

Hu, Lin; Li, Honglang; Li, Weifeng; Chen, Junmin; Yang, Jiantao; J, Gu; Xin, Lin

doi:10.3748/wjg.v23.i20.3721

Cited by 15 publications

(13 citation statements)

References 51 publications

(33 reference statements)

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“…It is well established that PCNA is a cell proliferation-associated protein and manifests its expression during the cell division of normal and neoplastic cells (24). The cell proliferation marker PCNA expression is associated with the genesis and development of a variety of cancer types, including breast cancer (25) and gastric cancer (26). In the present study, PCNA mRNA expression was upregulated in three breast cancer cell lines compared with MCF-10A cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

Novel lncRNA PSMG3‑AS1 functions as a miR‑143‑3p sponge to increase the proliferation and migration of breast cancer cells

et al. 2019

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Long non-coding RNAs (lncRNAs) are considered to be important regulators in breast cancer. In the present study, the potential mechanisms and functional roles of lncRNA PSMG3-antisense (AS)1 were investigated in vivo and in vitro. The relative expression levels of lncRNA PSMG3-AS1 and microRNA (miR)-143-3p were determined using reverse-transcription quantitative PCR. The protein expression levels of collagen type 1 alpha 1 (COL1A1) and proliferating cell nuclear antigen (PCNA) were obtained using western blot analysis. Bioinformatics analysis was used to identify the relationship between PSMG3-AS1, miR-143-3p and COL1A1. Colony forming and Cell Counting Kit-8 assays were used to detect cell proliferation. Transwell and wound-healing assays were used to determine cell migration. The results of the present study demonstrated that PSMG3-AS1 expression was increased in breast cancer tumor tissues and cell lines, and that of miR-143-3p was decreased. Knockdown of PSMG3-AS1 increased the level of miR-143-3p expression, which led to the mitigation of proliferation and migration capacity in breast carcinoma cells. Additionally, PSMG3-AS1 knockdown was demonstrated to reduce the mRNA and protein expression levels of COL1A1. miR-143-3p mimic transfection reduced proliferation and migration in MDA-MB-231 and MCF-7 cell lines. Furthermore, miR-143-3p inhibition significantly increased the proliferation and migration of breast cancer cells compared with the negative control group. The mRNA and protein expression levels of PCNA were reduced in the MCF-7 cell line when transfected with miR-143-3p mimics and si-PSMG3-AS1. However, PCNA expression was increased in cells transfected with a miR-143-3p inhibitor. In conclusion, the results of the present study identified a novel lncRNA PSMG3-AS1, which serves as a sponge for miR-143-3p in the pathogenesis of breast cancer. PSMG3-AS1 may be used as a potential therapeutic target gene in breast cancer treatment.

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Section: Resultsmentioning

confidence: 99%

Novel lncRNA PSMG3‑AS1 functions as a miR‑143‑3p sponge to increase the proliferation and migration of breast cancer cells

et al. 2019

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“…PCNA is a cell proliferation‐associated protein, which is also an essential component of the eukaryotic chromosomal DNA replisome . We found that the PCNA expression ascended by NE in normoxic and hypoxic condition, but the effect was reversed in the presence of 5‐MU.…”

Section: Discussionmentioning

confidence: 76%

alpha1A‐adrenoceptor is involved in norepinephrine‐induced proliferation of pulmonary artery smooth muscle cells via CaMKII signaling

Luo

Xiao-yan

Liu

et al. 2018

J of Cellular Biochemistry

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Pulmonary arterial hypertension (PAH) is a progressive disease of the pulmonary vasculature characterized by excessive proliferation of pulmonary artery smooth muscle cells (PASMCs). Some studies have demonstrated the sympathetic nervous system is activated in PAH and norepinephrine (NE) released is closely linked with its activation. However, the subtypes of adrenoreceptor (AR) and the downstream molecular cascades which are involved in the proliferation of PASMCs are still unclear. In this study, adult male Wistar rats were exposed to chronic hypoxia and PASMCs were cultured in hypoxic condition. Significant upregulation of α 1A -AR was identified by Western blot analysis or immunofluorescence in all of the pulmonary arteries, lung tissues, and cell hypoxic models. Western blot analysis, flow cytometry, and immunofluorescence were applied to detect the roles of α 1A -AR in NE mediated proliferation of PASMCs. We revealed 5-methylurapidil (5-MU) reversed NE-induced upregulation of PCNA, CyclinA and CyclinE, more cells from G 0 /G 1 phase to G 2 /M+S phase, enhancement of the microtubule formation. In addition, we found calcium/calmodulin(CaM)-dependent protein kinase type II (CaMKII) pathway was involved in α 1A -AR-mediated cell proliferation. [Ca 2+ ] i measurements showed that an increase of [Ca 2+ ] i caused by NE or/and hypoxia could be blocked by 5-MU in PASMCs. Western blot analysis results demonstrated the augmentation of CaMKII phosphorylation level was caused by hypoxia or NE in pulmonary arteries, lung tissues, and PASMCs.KN62 attenuated NE-induced proliferation of PASMCs under normoxia and hypoxia. In conclusion, those results suggested NE which stimulated α 1A -ARmediated the proliferation of PASMCs, which may be via the CaMKII pathway, and it could be used as a novel treatment strategy in PAH.J Cell Biochem. 2019;120:9345-9355.wileyonlinelibrary.com/journal/jcb

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“…Proliferating cell nuclear antigen (PCNA) plays a significant role in cancer cell growth and survival by regulating DNA synthesis and repair [ 51 ]. Previous studies showed that higher expression of PCNA correlated with prognosis of colon adenocarcinoma, osteosarcoma, and gastric carcinoma [ 52 – 54 ]. Moreover, PCNA is a potential therapeutic target for NSCLC [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance of epigenetic regulatory gene expression in patients with non-small-cell lung cancer

Chen

Tian

et al. 2021

Aging

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In this study, we used public databases to investigate the prognostic significance of epigenetic regulatory gene expression in patients with non small-cell lung cancer (NSCLC). Oncomine database analysis showed that the mRNA levels of seven epigenetic regulatory genes, UHRF1, EZH2, TTF2, SUV39H2, PCNA, WHSC1 and RAD54L , genes were significantly upregulated in NSCLC patients as compared to normal lung tissues. Functional enrichment analysis of these seven genes showed that the most enriched GO terms were DNA repair and rhythmic process, whereas, the most enriched KEGG pathway was lysine degradation pathway. The mRNA and protein expression levels of UHRF1, EZH2, TTF2, WHSC1 and RAD54L significantly correlated with tumor stage in NSCLC patients. Moreover, NSCLC patients exhibiting higher UHRF1, EZH2, WHSC1 and RAD54L mRNA and protein expression levels had poorer progression-free survival and overall survival. These findings demonstrate that UHRF1, EZH2, WHSC1 and RAD54L are potential prognostic biomarkers to distinguish high-risk from low-risk NSCLC patients.

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Clinical significance of expression of proliferating cell nuclear antigen and E-cadherin in gastric carcinoma

Cited by 15 publications

References 51 publications

Novel lncRNA PSMG3‑AS1 functions as a miR‑143‑3p sponge to increase the proliferation and migration of breast cancer cells

Novel lncRNA PSMG3‑AS1 functions as a miR‑143‑3p sponge to increase the proliferation and migration of breast cancer cells

alpha1A‐adrenoceptor is involved in norepinephrine‐induced proliferation of pulmonary artery smooth muscle cells via CaMKII signaling

Prognostic significance of epigenetic regulatory gene expression in patients with non-small-cell lung cancer

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