Novel lncRNA PSMG3‑AS1 functions as a miR‑143‑3p sponge to increase the proliferation and migration of breast cancer cells

Cui, Yue; Fan, Yuhua; Zhao, Guangcai; Zhang, Qibing; Bao, Ying; Cui, Yuanri; Ye, Zeng Jie; Chen, Guoyou; Piao, Xiang‐Lan; Guo, Fang; Wang, Jinghao; Bai, Yuhua; Yu, Dihua

doi:10.3892/or.2019.7390

Cited by 27 publications

(38 citation statements)

References 39 publications

(46 reference statements)

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“…The functionality of PSMG3-AS1 has only been investigated in breast cancer [13]. It is observed that PSMG3-AS1 is overexpressed in breast cancer and may promote the migration and proliferation of cancer cells by sponging miR-143-3p [13]. To the best of our knowledge, this study is the first to report the upregulation of PSMG3-AS1 in LUAD, a major subtype of lung cancer.…”

Section: Discussionmentioning

confidence: 93%

“…In addition, miR-449b-5p might be able to target PSMG3-AS1 to suppress cancer cell proliferation. The functionality of PSMG3-AS1 has only been investigated in breast cancer [13]. It is observed that PSMG3-AS1 is overexpressed in breast cancer and may promote the migration and proliferation of cancer cells by sponging miR-143-3p [13].…”

Section: Discussionmentioning

confidence: 99%

“…However, the functions of most ncRNAs in cancer remain unclear. PSMG3-AS1 was recently characterized as an oncogenic lncRNA in breast cancer [13], while its role in lung cancer remains unclear. Our bioinformatics analysis showed that PSMG3-AS1 may be targeted by miR-449b-5p, which is a tumor suppressive miRNA [14].…”

Section: Introductionmentioning

confidence: 99%

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MiR-449b-5p targets lncRNA PSMG3-AS1 to suppress cancer cell proliferation in lung adenocarcinoma

Zhang

et al. 2020

BMC Pulm Med

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Background: PSMG3-AS1 has been characterized as an oncogenic lncRNA in breast cancer, while its role in other cancers is unknown. This study investigated the role of PSMG3-AS1 in lung adenocarcinoma (LUAD). Methods: This study included 64 LUAD patients (42 males and 22 females) who were enrolled between May 2012 and May 2014. RT-qPCR was used to evaluate the expression levels of lncRNA. Cell proliferation analysis was performed using CCK-8 kit. Results: We found that upregulation of PSMG3-AS1 in LUAD predicted the poor survival of patients. MiR-449b-5p is downregulated in LUAD and the expression levels of LUAD were inversely correlated with the expression levels of PSMG3-AS1. MiR-449b-5p was predicted to target PSMG3-AS1, and overexpression of miR-449b-5p resulted in the downregulation of PSMG3-AS1 in LUAD cells. Cell proliferation analysis showed that overexpression of PSMG3-AS1 resulted in increased rate of cell proliferation. Overexpression of miR-449b-5p reduced the enhancing effects of PSMG3-AS1 on cell proliferation. Conclusions: Therefore, miR-449b-5p may target PSMG3-AS1 in LUAD to suppress cancer cell proliferation.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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MiR-449b-5p targets lncRNA PSMG3-AS1 to suppress cancer cell proliferation in lung adenocarcinoma

Zhang

et al. 2020

BMC Pulm Med

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“…Studies had shown that LncRNA was abnormally expressed in a variety of human cancers, and its abnormal expression was significantly related to the proliferation, migration, invasion of cancer cell and so on. For example, the expression of lncRNA PSMG3-AS1 was increased in breast cancer tissues and cells and Interference of PSMG3-AS1 expression can promote the expression of miR-143-3p, resulting in the weakness of proliferation and migration of breast cancer cells [5]. The expression of LINC00467 was up-regulated in HCC tissues and cells., and it could inhibit the proliferation and metastasis of HCC cells and promote apoptosis if LINC00467 was silenced [6].…”

mentioning

confidence: 98%

Long non-coding RNA HOXA-AS2 may serve as a new therapeutic target and promising prognostic market for most of cancers

Guo

Fang

Gao

et al. 2020

Preprint

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Backgroud: To elucidate the relationship between the expression level of HOXA-AS2 and it’s prognostic value of cancer by meta-analysis. Methods: Databases of PubMed, Cochrane Library, Embase, Web of Science, Google Scholar, CNKI and some others were searched systematically. Comprehensively screen according to the inclusion criteria and the exclusion criteria was conducted. The connection between HOXA-AS2 and the prognosis characteristics of patients with various types of cancer was screened by combining odds ratio (OR), Hazard ratios (HR) and 95% confidence interval (CI) for the studies collected in this meta-analysis. In addition, we further analyzed the expression of the gene and its potential clinical value in Gene Expression Profiling Interactive Analysis (GEPIA) and the lnCAR database. Results: A total of 12 studies consisting 796 patients were included in this research. Compared low HOXA-AS2 expression group, patients with high HOXA-AS2 expression were more likely to get poor overall survival (OS). Moreover, high HOXA-AS2 expression demonstrates advanced TNM stage, earlier lymph node metastasis, distant metastasis and bigger tumor size. But there was no correlation or the correlation was not statistically significant between the expression and the age, sex or the pathological differentiation. In addition, data from the GEPIA and LnCAR databases revealed that increasing HOXA-AS2 expression means bad prognosis in most of cancers. Conclusions: High HOXA-AS2 expression shows worse cancer prognosis in cancer patients, and HOXA-AS2 may be acted as therapeutic target and promising prognostic marker.

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“…In conclusion, LINC00221 suppressed ALL cell proliferation and boosted ALL cell apoptosis via sponging miR-152-3p to up-regulate ATP2A2. regulate COL1A1 expression, thus enhancing proliferation and migration ability of breast cancer cells [7]. COL1A1-014 functions as a ceRNA in gastric cancer development via up-regulating CXCL12 and CXCR4 through interacting with miR-1273h-5p [8].…”

mentioning

confidence: 99%

LINC00221 suppresses the malignancy of children acute lymphoblastic leukemia

Huang

Ren³

et al. 2020

Bioscience Reports

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Correspondence: Man Huang (huangman770@163.com)As the most common malignant disease in childhood, children acute lymphoblastic leukemia (ALL) is a heterogeneous disease caused by the accumulated genetic alterations. Long non-coding RNAs (lncRNAs) are reported as critical regulators in diseases. GEPIA database indicated that long intergenic non-protein coding RNA 221 (LINC00221) was conspicuously down-regulated in acute myeloid leukemia. However, its expression pattern in ALL has not been revealed. This work was carried out to study the role of LINC00221 in ALL cells. Quantitative real-time PCR (qRT-PCR) quantified LINC00221 expression in ALL cells. The function of LINC00221 in ALL was determined by ki-67 immunofluorescence staining, EdU, TUNEL, JC-1, and caspase-3/8/9 activity assays. RNA pull down and Ago2-RNA immunoprecipitation (RIP) assays investigated the interaction between miR-152-3p and LINC00221 or ATPase sarcoplasmic/endoplasmic reticulum Ca2 + transporting 2 (ATP2A2). Our study revealed the low expression of LINC00221 in ALL cells. Subsequently, LINC00221 was verified to bind with miR-152-3p. Moreover, functional assays pointed out that LINC00221 overexpression posed anti-proliferation and pro-apoptosis effects in ALL cells, and these effects could be separately reversed by miR-152-3p up-regulation. Afterward, LINC00221 was revealed to regulate ATP2A2 expression via sponging miR-152-3p. Additionally, ATP2A2 was verified to involve in regulating LINC00221-mediated ALL cell proliferation and apoptosis. In conclusion, LINC00221 suppressed ALL cell proliferation and boosted ALL cell apoptosis via sponging miR-152-3p to up-regulate ATP2A2. regulate COL1A1 expression, thus enhancing proliferation and migration ability of breast cancer cells [7]. COL1A1-014 functions as a ceRNA in gastric cancer development via up-regulating CXCL12 and CXCR4 through interacting with miR-1273h-5p [8]. Up-regulation of lncRNA HOTAIR promotes PRAF2 expression via acting as a sponge of miR-326 in cutaneous squamous cell carcinoma [9]. LncRNA NEAT1 contributes to lung cancer cell proliferation and invasion via NEAT1/miR-1224/KLF3 ceRNA pattern [10].Long intergenic non-coding RNA 00221 (LINC00221) was reported to enhance the cisplatin resistance of non-small cell lung cancer [11]. Also, LINC00221 is a prognostic biomarker for patients with hepatocellular carcinoma [12]. Besides, GEPIA database (http://gepia.cancer-pku.cn/) indicated that LINC00221 was conspicuously down-regulated in acute myeloid leukemia. However, the expression pattern and functional role of LINC00221 in ALL are still uncovered. Thus, the study was designed to explore the function and underlying mechanism of LINC00221 in ALL cells. Materials and methods Cell cultureHuman ALL cell lines (Jurkat, CCRF-CEM, CEM/C1, 1301) and normal HEK 293T cell line were available from the ATCC (Manassas, VA) and cultivated in 5% CO 2 at 37 • C. Cell samples were all maintained in RPMI-1640 medium, with 10% FBS (HyClone, Logan, UT) and 1% penicillin-streptomycin as supplements. RNA...

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Novel lncRNA PSMG3‑AS1 functions as a miR‑143‑3p sponge to increase the proliferation and migration of breast cancer cells

Cited by 27 publications

References 39 publications

MiR-449b-5p targets lncRNA PSMG3-AS1 to suppress cancer cell proliferation in lung adenocarcinoma

MiR-449b-5p targets lncRNA PSMG3-AS1 to suppress cancer cell proliferation in lung adenocarcinoma

Long non-coding RNA HOXA-AS2 may serve as a new therapeutic target and promising prognostic market for most of cancers

LINC00221 suppresses the malignancy of children acute lymphoblastic leukemia

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