Clinical Significance of Epidermal Growth Factor Receptor Content in Gastric Cancer

García, I; Vizoso, F; Andicoechea, Alejandro; Raigoso, P; P, Vérez; Alexandre, E; Jl, García-Muñíz; Mt, Allende

doi:10.1177/172460080101600305

Cited by 14 publications

(2 citation statements)

References 25 publications

(33 reference statements)

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“…Most gastric cancers overexpress both EGF and epidermal growth factor receptor (EGFR) [16,17] and EGF appears to be closely involved in growth of gastric carcinomas [18]. In addition to its effects on cell proliferation, there is some evidence that increased EGF or EGFR expression may be related to disruption of TJ in tumor cells.…”

Section: Introductionmentioning

confidence: 99%

EGF rapidly translocates tight junction proteins from the cytoplasm to the cell–cell contact via protein kinase C activation in TMK-1 gastric cancer cells

Kanaoka

Takai

Uezato

et al. 2005

Experimental Cell Research

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Tight junctions are commonly disrupted in cancer cells, including gastric cancer. Various growth factors have been reported to affect the localization of tight junction-associated proteins such as ZO-1 and occludin. We investigated the effect of epidermal growth factor (EGF), a growth factor that is often overexpressed in gastric cancer, and fetal bovine serum (FBS) on the localization of ZO-1 and occludin in a gastric cancer cell line. In the poorly differentiated gastric cancer cell line TMK-1, immunohistochemistry demonstrated that ZO-1 and occludin were predominantly localized to the cytoplasm, although there was some weak expression at the cell-cell contact. When the medium was replaced with fresh medium containing 10% FBS, ZO-1 and occludin were rapidly translocated from the cytosol to the cell-cell contact. A similar effect was seen in EGF exposure. These effects induced by FBS or EGF were attenuated in the presence of protein kinase C (PKC) inhibitors calphostin C and bisindolylmaleimide I, but not another PKC inhibitor Gö6976, PD98059 (MAPK inhibitor), LY294002 (PI3 kinase inhibitor) or KT5720 (protein kinase A inhibitor). These results suggest that serum-derived factors, including EGF, can rapidly alter the localization of ZO-1 and occludin via a protein kinase C signaling pathway in TMK-1 gastric cancer cells.

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Section: Introductionmentioning

confidence: 99%

EGF rapidly translocates tight junction proteins from the cytoplasm to the cell–cell contact via protein kinase C activation in TMK-1 gastric cancer cells

Kanaoka

Takai

Uezato

et al. 2005

Experimental Cell Research

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“…Epidermal growth factor (EGF) promotes the growth of cells from both an ectodermal and mesodermal origin, and EFG plays an important role in cellular proliferation, differentiation and tumor progression of human gastric carcinomas (4). The EGF receptor/ligand system seems to be involved in the regulation of gastric mucosa proliferation and growth regulation of gastric carcinomas (5).…”

Section: Introductionmentioning

confidence: 99%

Prognostic Significance of Immunohistochemical Expression of EGFR and C-erbB-2 Oncoprotein in Curatively Resected Gastric Cancer

et al. 2004

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Purpose: T he purpose of this study was to investigate the prognostic significance of the expre ssion of E G F R and C -erbB-2 gene products by im m unohistochem ical analysis for cura tively re sected ga stric adenocarcinom a.M a terials and M ethods: Between January 1996 and D ece m be r 2 001 , 7 39 pa tients with cura tively resecte d ga stric cancer pa tients underwent im m unohistoche m ical staining for E G F R a nd C -e rbB-2 proteins, and we retrospe ctive ly a na lyze d the ir corre la tion with the clinica l outcom e .Results: The overexpressions of EGFR and C-erbB-2 were 25.4% and 26.2% , respectively. The overexpressions of EGFR was associated with the more poorly differentiated tumor (p=0.000) and with neuronal invasion (p=0.03). O verexpression of C-erbB-2 was associated with less vascular invasion (p=0.001). Tumor depth or node metastasis was not related to the overexpression of E G FR or C -erbB-2. The seven-year overall survival and re la pse -fre e surviva l ra te s we re 8 7 .2 % a nd 7 5 .8 % , respectively. U pon multivariate C ox regression analysis, the tum or sta ge , tum or size a nd pa tie nt a ge we re im porta nt prognostic fa ctors for overa ll survival, and tum or stage wa s the im porta nt fa ctor for re la pse -fre e surviva l. O ve re xpressions of E G F R or c-erbB-2 were not significant prognostic factors.C onclusion: Im m unohistochem ica l sta ining of E G F R and C -erbB-2 gene products were not independent prognostic fa ctors for pre dicting the ove ra ll surviva l a nd the relapse-free survival in curatively resected gastric cancer.

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Are biomarkers correlated with recurrence patterns in patients with resectable gastric adenocarcinoma

Zhang

Xue

et al. 2011

Mol Biol Rep

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The aims of this study are to analyze the failure patterns in radical resected gastric adenocarcinoma, and to evaluate the correlation between recurrence patterns and potentially prognostic factors, including clinical pathological characteristic and biomarkers. Between Jan 2004 and Jun 2006, 84 patients were enrolled into the database analysis, including 8 with clinical stage I, 20 with clinical stage II, 21 with clinical stage IIIA, 22 with clinical stage IIIB and 13 with clinical stage IV, male 61 and female 23. The collected biomarkers including: preoperative tumor markers: CEA, AFP, CA199, CA50, CA72-4 and CA24-2; postoperative immunohistochemical (IHC) markers: Bax, Bcl-2, P27, CyclinD1, TOPO2, MDR, GST-π, Ki67, epidermal growth factor receptor (EGFR), P21, P53, proliferating cell nuclear antigen (PCNA), C-myc and Neu. Three-year local control rate (LCR), disease-free survival (DFS) and over-all survival (OS) were 66, 61 and 64% respectively. Logistic regression analysis showed cyclinD1 and CEA were correlated with prognosis; cyclinD1, CEA were correlated with loco-regional recurrence; PCNA was correlated with remote metastasis; bcl-2, ki67, c-myc2 and Neu were correlated with lymph node metastasis. The present study indicate that patterns of recurrence are variable and may be associated with specific biomarkers, in addition, high level of CEA and low-expressed of cyclinD1 resulted in poor prognosis.

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Clinical Significance of Epidermal Growth Factor Receptor Content in Gastric Cancer

Cited by 14 publications

References 25 publications

EGF rapidly translocates tight junction proteins from the cytoplasm to the cell–cell contact via protein kinase C activation in TMK-1 gastric cancer cells

EGF rapidly translocates tight junction proteins from the cytoplasm to the cell–cell contact via protein kinase C activation in TMK-1 gastric cancer cells

Prognostic Significance of Immunohistochemical Expression of EGFR and C-erbB-2 Oncoprotein in Curatively Resected Gastric Cancer

Are biomarkers correlated with recurrence patterns in patients with resectable gastric adenocarcinoma

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