Clinical significance of c- kit expression in biliary atresia

Ahmed, Abul Faiz Kabir Uddin; Nio, Masaki; Ohtani, Haruo; Nishimura, Hiroshi; Ohi, Ryoji

doi:10.1007/s003830100002

Cited by 5 publications

(6 citation statements)

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“…Biliary atresia is still the leading cause of liver transplantation (LT) for children [1][2][3][4][5]. The fibrogenic mechanism in BA may include activation of hepatic stellate cells and increase of extracellular matrix (ECM)-a complex process involving many cytokines of which a few have been identified [6][7][8][9][10][11][12][13].…”

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confidence: 99%

Evaluation of matrix metalloproteinases and their endogenous tissue inhibitors in biliary atresia–associated liver fibrosis

Hsieh

Chuang

Huang

et al. 2005

Journal of Pediatric Surgery

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confidence: 99%

Evaluation of matrix metalloproteinases and their endogenous tissue inhibitors in biliary atresia–associated liver fibrosis

Hsieh

Chuang

Huang

et al. 2005

Journal of Pediatric Surgery

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“…[6][7][8][9][10][11][12] The extracellular matrix holds cells together and maintains the three-dimensional structure of organs including the liver. The extracellular matrix component of the normal liver is relatively minor, and liver fibrosis is the result of extensive tissue remodeling with a net increase in extracellular matrix.…”

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confidence: 99%

Matrilysin (MMP-7) is a major matrix metalloproteinase upregulated in biliary atresia-associated liver fibrosis

et al. 2005

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Matrix metalloproteinases (MMPs) are the proteases responsible for tissue remodeling during liver fibrosis caused by various disorders including biliary atresia. However, information regarding the relative contribution of these proteases to liver fibrosis is still limited. We studied matrix metalloproteinase-2 (MMP-2), -7, -9 and -13 mRNA expressions in the liver tissue of early-stage biliary atresia at the time of Kasai's procedure, late-stage biliary atresia at the time of liver transplantation with advanced fibrosis and nondiseased control without liver fibrosis. The results of real-time quantitative reverse transcriptase-PCR analysis revealed that only MMP-2 and -7 expressions were significantly different between groups. MMP-2 was significantly higher in Liver Transplantation group than both in Control (P ¼ 0.010) and in Kasai's Procedure (P ¼ 0.001) groups, whereas the difference of MMP-2 expression between Control and Kasai's Procedure was not significant. However, the relative expression level of MMP-7 was sequentially elevated when comparing Control, Kasai's Procedure and Liver Transplantation groups, and there was significant (P ¼ 0.019) difference when comparing Control and Liver Transplantation groups. Moreover, the fold difference in MMP-7 mRNA was much higher than that in MMP-2 mRNA between groups. The expressions of MMP-7 were further confirmed by agarose gel electrophoresis and Western blotting. Immunohistochemical analysis revealed a significant positive correlation of the scores of MMP-7 immunostaining with the stages of liver fibrosis. In situ hybridization demonstrated that the bile ductular epithelial cells, Kupffer cells and hepatocytes were the major producers of matrix metalloproteinase-7 in the liver. Our results imply that MMP-7 is a major MMP associated with the tissue remodeling during the progression of liver fibrosis in biliary atresia. Modern Pathology (2005) 18, 941-950.

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“…SCF plays a key role in the recruitment of mast cells as well as in their activation 6 . In addition, recent evidence demonstrates the involvement of mast cells in the pathogenesis of BA 25,26 . Periportal accumulation of intrahepatic mast cells is a common feature of BA 25 .…”

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confidence: 99%